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kod -plus  (Toyobo)


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    Structured Review

    Toyobo kod -plus
    Kod Plus, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 6723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptome+shotgun+assembly+sequence+database/KOD+-Plus/custom%40kod-201%4026032664
    Average 96 stars, based on 6723 article reviews
    kod -plus - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Precision base-editing of the cryptic 3' acceptor site to correct the RNA splicing defect of β 654 -thalassemia.
    Article Snippet: .. Flanking sequences covering about 100 bp upstream and downstream of each on-target and predicted offtarget locus were amplified by KOD high-fidelity DNA polymerase (TOYOBO). ..

    Article Title: TAK1 Impedes Peripheral Nerve Regeneration After Injury by Evoking Sustained Inflammation.
    Article Snippet: .. Adeno-associated Virus (AAV) Vector Construction and Virus Production The full-length coding sequence of murine Map3k7 was amplified by PCR using KOD Plus DNA polymerase (KOD-201; Toyobo, Osaka, Japan) with the following primers: 5′-GAT ATC GTA CAT ATG TCG GTA CCG CCA CCA TGT CGA CAG CCT CCG CCG -3′ (forward) and 5′-AGA GGT TGA TTA TCG ATA AGC TTT CAT TTG TCG TCA TCA TCC TTA TAG TCC TTA -3′ (reverse). .. The shuttle vector pHBAAV-CMV-MCS-3flag-EF1-ZsGreen (Hanbio Biotechnology, Jo urn al Pr e-p roo f Shanghai, China) was linearized by restriction enzyme digestion using HindIII and EcoRV at 37°C for 2 h, followed by gel purification.

    Virus:

    Article Title: TAK1 Impedes Peripheral Nerve Regeneration After Injury by Evoking Sustained Inflammation.
    Article Snippet: .. Adeno-associated Virus (AAV) Vector Construction and Virus Production The full-length coding sequence of murine Map3k7 was amplified by PCR using KOD Plus DNA polymerase (KOD-201; Toyobo, Osaka, Japan) with the following primers: 5′-GAT ATC GTA CAT ATG TCG GTA CCG CCA CCA TGT CGA CAG CCT CCG CCG -3′ (forward) and 5′-AGA GGT TGA TTA TCG ATA AGC TTT CAT TTG TCG TCA TCA TCC TTA TAG TCC TTA -3′ (reverse). .. The shuttle vector pHBAAV-CMV-MCS-3flag-EF1-ZsGreen (Hanbio Biotechnology, Jo urn al Pr e-p roo f Shanghai, China) was linearized by restriction enzyme digestion using HindIII and EcoRV at 37°C for 2 h, followed by gel purification.

    Bioprocessing:

    Article Title: TAK1 Impedes Peripheral Nerve Regeneration After Injury by Evoking Sustained Inflammation.
    Article Snippet: .. Adeno-associated Virus (AAV) Vector Construction and Virus Production The full-length coding sequence of murine Map3k7 was amplified by PCR using KOD Plus DNA polymerase (KOD-201; Toyobo, Osaka, Japan) with the following primers: 5′-GAT ATC GTA CAT ATG TCG GTA CCG CCA CCA TGT CGA CAG CCT CCG CCG -3′ (forward) and 5′-AGA GGT TGA TTA TCG ATA AGC TTT CAT TTG TCG TCA TCA TCC TTA TAG TCC TTA -3′ (reverse). .. The shuttle vector pHBAAV-CMV-MCS-3flag-EF1-ZsGreen (Hanbio Biotechnology, Jo urn al Pr e-p roo f Shanghai, China) was linearized by restriction enzyme digestion using HindIII and EcoRV at 37°C for 2 h, followed by gel purification.

    Sequencing:

    Article Title: TAK1 Impedes Peripheral Nerve Regeneration After Injury by Evoking Sustained Inflammation.
    Article Snippet: .. Adeno-associated Virus (AAV) Vector Construction and Virus Production The full-length coding sequence of murine Map3k7 was amplified by PCR using KOD Plus DNA polymerase (KOD-201; Toyobo, Osaka, Japan) with the following primers: 5′-GAT ATC GTA CAT ATG TCG GTA CCG CCA CCA TGT CGA CAG CCT CCG CCG -3′ (forward) and 5′-AGA GGT TGA TTA TCG ATA AGC TTT CAT TTG TCG TCA TCA TCC TTA TAG TCC TTA -3′ (reverse). .. The shuttle vector pHBAAV-CMV-MCS-3flag-EF1-ZsGreen (Hanbio Biotechnology, Jo urn al Pr e-p roo f Shanghai, China) was linearized by restriction enzyme digestion using HindIII and EcoRV at 37°C for 2 h, followed by gel purification.

    Polymerase Chain Reaction:

    Article Title: TAK1 Impedes Peripheral Nerve Regeneration After Injury by Evoking Sustained Inflammation.
    Article Snippet: .. Adeno-associated Virus (AAV) Vector Construction and Virus Production The full-length coding sequence of murine Map3k7 was amplified by PCR using KOD Plus DNA polymerase (KOD-201; Toyobo, Osaka, Japan) with the following primers: 5′-GAT ATC GTA CAT ATG TCG GTA CCG CCA CCA TGT CGA CAG CCT CCG CCG -3′ (forward) and 5′-AGA GGT TGA TTA TCG ATA AGC TTT CAT TTG TCG TCA TCA TCC TTA TAG TCC TTA -3′ (reverse). .. The shuttle vector pHBAAV-CMV-MCS-3flag-EF1-ZsGreen (Hanbio Biotechnology, Jo urn al Pr e-p roo f Shanghai, China) was linearized by restriction enzyme digestion using HindIII and EcoRV at 37°C for 2 h, followed by gel purification.

    Article Title: Multiplex LAMP-LFA for rapid field-compatible detection of environmental DNA from Schistosoma japonicum and Oncomelania hupensis quadrasi
    Article Snippet: .. Amplifications were performed in 50 μL reaction volumes using the KOD Plus PCR Master Mix (TOYOBO, Japan) following the manufacturer’s recommended reaction concentrations. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: TAK1 Impedes Peripheral Nerve Regeneration After Injury by Evoking Sustained Inflammation.
    Article Snippet: .. Adeno-associated Virus (AAV) Vector Construction and Virus Production The full-length coding sequence of murine Map3k7 was amplified by PCR using KOD Plus DNA polymerase (KOD-201; Toyobo, Osaka, Japan) with the following primers: 5′-GAT ATC GTA CAT ATG TCG GTA CCG CCA CCA TGT CGA CAG CCT CCG CCG -3′ (forward) and 5′-AGA GGT TGA TTA TCG ATA AGC TTT CAT TTG TCG TCA TCA TCC TTA TAG TCC TTA -3′ (reverse). .. The shuttle vector pHBAAV-CMV-MCS-3flag-EF1-ZsGreen (Hanbio Biotechnology, Jo urn al Pr e-p roo f Shanghai, China) was linearized by restriction enzyme digestion using HindIII and EcoRV at 37°C for 2 h, followed by gel purification.



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    Image Search Results


    Cumulative length (A) and sorted transcripts length (B) of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet: Cumulative length (A) and sorted transcripts length (B) of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are openly accessible at Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques:

    Completeness scores of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet: Completeness scores of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are openly accessible at Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques:

    Summary of ORFs predicted in the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet: Summary of ORFs predicted in the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are openly accessible at Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques:

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet:

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are openly accessible at Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques: Sequencing, Functional Assay, Purification, Cell Culture, Construct, Software, Isolation

    Cumulative length (A) and sorted transcripts length (B) of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet: Cumulative length (A) and sorted transcripts length (B) of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are hosted in the public repository Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques:

    Completeness scores of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet: Completeness scores of the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are hosted in the public repository Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques:

    Summary of ORFs predicted in the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet: Summary of ORFs predicted in the de novo assembled transcriptomes of the native oleaginous microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp.

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are hosted in the public repository Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques:

    Journal: Data in Brief

    Article Title: Dataset of de novo assembly and functional annotation of the transcriptomes of three native oleaginous microalgae from the Peruvian Amazon

    doi: 10.1016/j.dib.2020.105917

    Figure Lengend Snippet:

    Article Snippet: , Also, transcriptome shotgun assembly sequences database (fasta.gz format) and functional annotation results are hosted in the public repository Discover Mendeley Data ( https://data.mendeley.com/datasets/47wdjmw9xr/1 )..

    Techniques: Sequencing, Functional Assay, Purification, Cell Culture, Construct, Software, Isolation