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library construction and transcriptome sequencing  (Novogene)

 
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    Novogene library construction and transcriptome sequencing
    Library Construction And Transcriptome Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptome+library+construction/transcriptome+sequencing/pm40650032-204-4-10
    Average 90 stars, based on 1 article reviews
    library construction and transcriptome sequencing - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Establishment of a humanized SCA2 mouse model carrying a CAA disruption preventing CAG repeat expansion in pathogenic genes.
    Article Snippet: .. Transcriptome sequencing and partial analysis were performed by Beijing Novogene Bioinformatics Technology Co., Ltd. ..

    Article Title: Transcriptome reveals the role of ELONGATED HYPOCOTYL 5 in the regulation of glucosinolate metabolism in broccoli
    Article Snippet: .. Transcriptome sequencing, library construction, and data pre-processing were performed by Novogene Bioinformatics Technology Co. Ltd. RNA-seq libraries were constructed and sequenced on an Illumina NovaSeq 6000 platform using paired-end mode, with three biological replicates for each treatment. ..

    Article Title: Transcriptome- and Metabolome-Based Regulation of Growth, Development, and Bioactive Compounds in Salvia miltiorrhiza (Lamiaceae) Seedlings by Different Phosphorus Levels.
    Article Snippet: The transcriptome libraries were constructed using the VAHTS Universal V5 RNA-seq Library Prep kit according to the instructions, and sequenced using the Illumina Hiseq 2000 sequencing platform. .. The library construction and transcriptome sequencing were performed by Beijing Novogene Bioinformatics Technology Co., Ltd. (Beijing, China). ..

    Article Title: Abscisic acid application enhances cadmium accumulation in Sedum plumbizincicola by controlling photosynthesis, hormone signaling and antioxidant defense.
    Article Snippet: Sedum plumbizincicola (S. plumbizincicola) is a hyperaccumulator species with high potential for the remediation of cadmium (Cd)-contaminated lands.. The importance of abscisic acid (ABA) in regulating phytoremediation processes has been widely recognized, but the physiological and molecular mechanisms underlying ABAmediated Cd uptake and detoxification in hyperaccumulators remain unclear.. Here, we investigated how ABA application at low and high rates influenced Cd accumulation and tolerance in S. plumbizincicola, and explored the causal mechanisms.

    Article Title: A novel pilus-associated gene cluster is implicated in Streptococcus agalactiae virulence.
    Article Snippet: .. The lysate was collected and sent to Beijing Novogene Technology Co., Ltd., for transcriptome sequencing and analysis. ..

    Article Title: CCAAT/Enhancer-Binding Proteins α and β Regulate Ovulation and Gene Expression via Dose- and Stage-Dependent Mechanisms
    Article Snippet: .. Library preparation and transcriptome sequencing were conducted by Novogene Co, Ltd. ..

    Article Title: Exploring the additive antibacterial potential of Cinnamomum cassia volatile oil and imipenem against Acinetobacter baumannii : a multi-omics investigation
    Article Snippet: .. Samples were sent to Novogene Co., Ltd. (Beijing, China) for transcriptome sequencing. ..

    RNA Sequencing:

    Article Title: Transcriptome reveals the role of ELONGATED HYPOCOTYL 5 in the regulation of glucosinolate metabolism in broccoli
    Article Snippet: .. Transcriptome sequencing, library construction, and data pre-processing were performed by Novogene Bioinformatics Technology Co. Ltd. RNA-seq libraries were constructed and sequenced on an Illumina NovaSeq 6000 platform using paired-end mode, with three biological replicates for each treatment. ..

    Construct:

    Article Title: Transcriptome reveals the role of ELONGATED HYPOCOTYL 5 in the regulation of glucosinolate metabolism in broccoli
    Article Snippet: .. Transcriptome sequencing, library construction, and data pre-processing were performed by Novogene Bioinformatics Technology Co. Ltd. RNA-seq libraries were constructed and sequenced on an Illumina NovaSeq 6000 platform using paired-end mode, with three biological replicates for each treatment. ..

    other:

    Article Title: LAMC3 interference reduces drug resistance of carboplatin-resistant ovarian cancer cells.
    Article Snippet: Transcriptome sequenceing of was completed at Beijing Novogene Corporation.

    Incubation:

    Article Title: Abscisic acid application enhances cadmium accumulation in Sedum plumbizincicola by controlling photosynthesis, hormone signaling and antioxidant defense.
    Article Snippet: Sedum plumbizincicola (S. plumbizincicola) is a hyperaccumulator species with high potential for the remediation of cadmium (Cd)-contaminated lands.. The importance of abscisic acid (ABA) in regulating phytoremediation processes has been widely recognized, but the physiological and molecular mechanisms underlying ABAmediated Cd uptake and detoxification in hyperaccumulators remain unclear.. Here, we investigated how ABA application at low and high rates influenced Cd accumulation and tolerance in S. plumbizincicola, and explored the causal mechanisms.

    Control:

    Article Title: Abscisic acid application enhances cadmium accumulation in Sedum plumbizincicola by controlling photosynthesis, hormone signaling and antioxidant defense.
    Article Snippet: Sedum plumbizincicola (S. plumbizincicola) is a hyperaccumulator species with high potential for the remediation of cadmium (Cd)-contaminated lands.. The importance of abscisic acid (ABA) in regulating phytoremediation processes has been widely recognized, but the physiological and molecular mechanisms underlying ABAmediated Cd uptake and detoxification in hyperaccumulators remain unclear.. Here, we investigated how ABA application at low and high rates influenced Cd accumulation and tolerance in S. plumbizincicola, and explored the causal mechanisms.

    RNA Extraction:

    Article Title: Abscisic acid application enhances cadmium accumulation in Sedum plumbizincicola by controlling photosynthesis, hormone signaling and antioxidant defense.
    Article Snippet: Sedum plumbizincicola (S. plumbizincicola) is a hyperaccumulator species with high potential for the remediation of cadmium (Cd)-contaminated lands.. The importance of abscisic acid (ABA) in regulating phytoremediation processes has been widely recognized, but the physiological and molecular mechanisms underlying ABAmediated Cd uptake and detoxification in hyperaccumulators remain unclear.. Here, we investigated how ABA application at low and high rates influenced Cd accumulation and tolerance in S. plumbizincicola, and explored the causal mechanisms.



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    Image Search Results


    Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: Clinical Proteomics

    Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: