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library construction and transcriptomics analysis  (Denovo Biotechnology)

 
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    Denovo Biotechnology library construction and transcriptomics analysis
    Library Construction And Transcriptomics Analysis, supplied by Denovo Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptome+library+construction/library+construction+and+sequencing/pm40022884-101-4-10
    Average 90 stars, based on 1 article reviews
    library construction and transcriptomics analysis - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Genome-Wide Identification of Freezing-Responsive Genes in a Rapeseed Line NTS57 Tolerant to Low-Temperature
    Article Snippet: The construction of the library and subsequent sequencing were conducted by Gene Denovo Biotechnology Co., based in Guangzhou, China, utilizing the Illumina HiSeqTM 2500 platform.

    Article Title: CXCL16/CXCR6/TGF-β Feedback Loop Between M-MDSCs and Treg Inhibits Anti-Bacterial Immunity During Biofilm Infection.
    Article Snippet: Synovial RNA samples were sent to Gene Denovo Biotechnology in Guangzhou, China for library creation and sequencing.

    Article Title: Comparative transcriptomic analysis reveals the reactive oxygen species metabolism involving in melatonin-alleviated chilling injury in postharvest banana fruit.
    Article Snippet: Banana fruit is susceptible to chilling injury (CI) during cold storage, which causes quality deterioration and commodity value decline.. Here, the effects of melatonin on CI alleviation and reactive oxygen species (ROS) metabolism in postharvest banana were studied.. The results displayed that 500 μmol L 1 melatonin treatment effectively inhibited CI development in cold-stored banana during the 96 h of storage.

    Article Title: The Overexpression of SlPLATZ17 Can Increase the Tolerance of Tomatoes to Drought and Salt Stress.
    Article Snippet: After the RNA was extracted, it was sent to Gene Denovo Biotechnology for subsequent library construction and sequencing.

    RNA Extraction:

    Article Title: Preventive and controlling effects of N -acetylneuraminic acid in regulating glucose and lipid metabolism disorders via the gut-liver axis in high-fat diet mice.
    Article Snippet: Following OTU determination, the gut microbiota indices were assessed using the Omicsmart platform (Gene Denovo Biotechnology Co., Ltd Guangzhou, China; https://www.omicsmart.com). .. RNA extraction, construction of a sequencing library, and transcriptome sequencing were carried out by Gene Denovo Biotechnology Co. (Guangzhou, China). .. A trizol reagent kit (Invitrogen, Carlsbad, CA, USA) was used to extract the total RNA.

    Sequencing:

    Article Title: Preventive and controlling effects of N -acetylneuraminic acid in regulating glucose and lipid metabolism disorders via the gut-liver axis in high-fat diet mice.
    Article Snippet: Following OTU determination, the gut microbiota indices were assessed using the Omicsmart platform (Gene Denovo Biotechnology Co., Ltd Guangzhou, China; https://www.omicsmart.com). .. RNA extraction, construction of a sequencing library, and transcriptome sequencing were carried out by Gene Denovo Biotechnology Co. (Guangzhou, China). .. A trizol reagent kit (Invitrogen, Carlsbad, CA, USA) was used to extract the total RNA.

    Article Title: rs-TAC PDC, a peptide drug-conjugate, for targeted delivery of tacrolimus and sericin alleviates podocyte injury in diabetic nephropathy
    Article Snippet: Despite intensive glucose control or blood pressure control, the remission of proteinuria in patients with diabetic nephropathy remains extremely low.. Tacrolimus has been successful in treating immune-related and geneticrelated nephropathy, providing a feasible method to reduce proteinuria and podocyte damage in patients with diabetic nephropathy.. However, doctors do not know how to avoid the risk of increased blood glucose levels and infection induced by tacrolimus.

    Article Title: A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice.
    Article Snippet: Subsequently, these libraries were sequenced on a NovaSeq6000 platform (Illumina, USA), following the manufacturer’s instructions, paired-end sequencing data with a length of 150 bp were obtained. .. Gene Denovo Biotechnology Co., Ltd. (Guangzhou, CHINA) performed RNA library construction and sequencing. ..

    Article Title: lncRNA-WAL Promotes Triple-Negative Breast Cancer Aggression by Inducing β-Catenin Nuclear Translocation
    Article Snippet: .. Then the remaining RNAs were detected by library construction and sequencing at Guangzhou Gene Denovo Biotechnology Corporation. .. The RNA immunoprecipitation sequencing (RIP-seq) was performed using Illumina Hiseq6000 platform.



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    Image Search Results


    Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: Clinical Proteomics

    Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: