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warmstart rtx reverse transcriptase  (New England Biolabs)


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    Structured Review

    New England Biolabs warmstart rtx reverse transcriptase
    Warmstart Rtx Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/WarmStart+RTx+Reverse+Transcriptase/us11813604-238-83-87
    Average 96 stars, based on 230 article reviews
    warmstart rtx reverse transcriptase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Crispr effector system based coronavirus diagnostics
    Article Snippet: .. For Step (2)—one-pot SHERLOCK detection reaction: Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs 10× Isothermal Amplification Buffer (B0374S), New England BioLabs, supplied with M0538L and M0380L 100 mM MgSO4 (B1003S), New England BioLabs, supplied with M0538L and M0380L 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L), New England BioLabs Taurine (86329-100G), Millipore Sigma AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. Guide RNA for detecting N gene can be ordered from Synthego Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT (Optional) Proteinase K Inhibitor (539470-10 MG), Millipore Sigma.

    Article Title: DNA polymerase, aptamer, warm-start DNA polymerase and preparation methods and application thereof
    Article Snippet: .. RT-LAMP: 5 U WarmStart RTx reverse transcriptase (NEB), 100 nM aptamer-based warm-start DNA polymerase and 0.5×gelgreen were added to a RT-LAMP reaction system. ..

    Article Title: High throughput influenza A virus detection by isothermal amplification in sequential-injection paper-based microfluidics.
    Article Snippet: The recognized impact of epidemics and pandemics caused by Influenza A virus highlights the need for rapid, sensitive, and affordable diagnostic methods.. In this work, we propose a molecular detection strategy for Influenza A viruses that combines Electrochemical reverse transcription Loop-Mediated Isothermal Amplification (E-RT-LAMP) using methylene blue (MB) as a redox-active probe, with detection carried out on a sequentialinjection paper-based microfluidics (μPAD).. The high amplification efficiency of the LAMP technique, following specific target recognition, combined with the intercalation of MB into double-stranded DNA enabled label-free detection of the target sequence through current variation with μPAD.

    Article Title: Looped primer and loop-de-loop method for detecting target nucleic acid
    Article Snippet: .. In some embodiments, the reverse transcriptase is WarmStart® RTx Reverse Transcriptase (available from NEB). ..

    Article Title: A red/blue bicolor lateral flow immunoassay for simultaneous detection of two enteroviruses based on duplex RT-LAMP
    Article Snippet: .. Bst 2.0 WarmStart ® DNA Polymerase, MgSO 4 , WarmStart ® RTx Reverse Transcriptase, 10 × isothermal amplification buffer, and LAMP fluorescent dyes were purchased from New England Biolabs (Ipswich, MA, United States). .. Thermally unstable uracil-DNA glycosylase (UDG) and dNTPs were obtained from Vazyme Biotech Co., Ltd. (Nanjing, China).

    Article Title: DNA polymerase, aptamer, warm-start DNA polymerase and preparation methods and application thereof
    Article Snippet: .. The amplification reaction was performed at 65° C. on a real-time PCR system of QuantStudio 7 Flex (Thermo Scientific). pH mediated colorimetric RT-LAMP: 5 U WarmStart RTx Reverse Transcriptase (NEB), and 100 nM aptamer-based warm-start DNA polymerase were added in a RT-LAMP reaction system. ..

    Article Title: High throughput reaction assembly
    Article Snippet: In the examples, a thermostable reverse transcriptase commercially available having the name Luna® RT (New England Biolabs, Ipswich, MA) was used. .. Although a single reverse transcriptase is exemplified below, other transcriptases that are commercially available such as SuperScriptTM RT (ThermoFisher Scientific, Waltham, MA), iScriptTM RT (BioRad, Hercules, CA), WarmStart® RTx and ProtoScript® RT (New England Biolabs, Ipswich, MA). ..

    Amplification:

    Article Title: Crispr effector system based coronavirus diagnostics
    Article Snippet: .. For Step (2)—one-pot SHERLOCK detection reaction: Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs 10× Isothermal Amplification Buffer (B0374S), New England BioLabs, supplied with M0538L and M0380L 100 mM MgSO4 (B1003S), New England BioLabs, supplied with M0538L and M0380L 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L), New England BioLabs Taurine (86329-100G), Millipore Sigma AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. Guide RNA for detecting N gene can be ordered from Synthego Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT (Optional) Proteinase K Inhibitor (539470-10 MG), Millipore Sigma.

    Article Title: A red/blue bicolor lateral flow immunoassay for simultaneous detection of two enteroviruses based on duplex RT-LAMP
    Article Snippet: .. Bst 2.0 WarmStart ® DNA Polymerase, MgSO 4 , WarmStart ® RTx Reverse Transcriptase, 10 × isothermal amplification buffer, and LAMP fluorescent dyes were purchased from New England Biolabs (Ipswich, MA, United States). .. Thermally unstable uracil-DNA glycosylase (UDG) and dNTPs were obtained from Vazyme Biotech Co., Ltd. (Nanjing, China).

    Article Title: DNA polymerase, aptamer, warm-start DNA polymerase and preparation methods and application thereof
    Article Snippet: .. The amplification reaction was performed at 65° C. on a real-time PCR system of QuantStudio 7 Flex (Thermo Scientific). pH mediated colorimetric RT-LAMP: 5 U WarmStart RTx Reverse Transcriptase (NEB), and 100 nM aptamer-based warm-start DNA polymerase were added in a RT-LAMP reaction system. ..

    Purification:

    Article Title: Crispr effector system based coronavirus diagnostics
    Article Snippet: .. For Step (2)—one-pot SHERLOCK detection reaction: Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs 10× Isothermal Amplification Buffer (B0374S), New England BioLabs, supplied with M0538L and M0380L 100 mM MgSO4 (B1003S), New England BioLabs, supplied with M0538L and M0380L 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L), New England BioLabs Taurine (86329-100G), Millipore Sigma AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. Guide RNA for detecting N gene can be ordered from Synthego Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT (Optional) Proteinase K Inhibitor (539470-10 MG), Millipore Sigma.

    Real-time Polymerase Chain Reaction:

    Article Title: DNA polymerase, aptamer, warm-start DNA polymerase and preparation methods and application thereof
    Article Snippet: .. The amplification reaction was performed at 65° C. on a real-time PCR system of QuantStudio 7 Flex (Thermo Scientific). pH mediated colorimetric RT-LAMP: 5 U WarmStart RTx Reverse Transcriptase (NEB), and 100 nM aptamer-based warm-start DNA polymerase were added in a RT-LAMP reaction system. ..



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