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sybr primescript rtpcr kit ii  (TaKaRa)


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    Structured Review

    TaKaRa sybr primescript rtpcr kit ii
    Sybr Primescript Rtpcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sybr+primescript+rtpcr+kit/PrimeScript+RT-PCR+Kit/pmc12300916-56-0-5
    Average 98 stars, based on 10903 article reviews
    sybr primescript rtpcr kit ii - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: Cellular mRNA was extracted using the RNeasy Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. .. Reverse transcription and subsequent qPCR were performed using the One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time; cat. no. RR066A; Takara Bio, Inc.), which includes TB Green as the fluorophore, according to the manufacturer's protocol. .. RT-qPCR was performed using the Thermal Cycler Dice Real Time System II (Takara Bio, Inc.) following the manufacturer's instructions.

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: Cellular mRNA was extracted using the RNeasy Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. .. Reverse transcription and subsequent qPCR were performed using the One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time; cat. no. RR066A; Takara Bio, Inc.), which includes TB Green as the fluorophore, according to the manufacturer's protocol. .. RT-qPCR was performed using the Thermal Cycler Dice Real Time System II (Takara Bio, Inc.) following the manufacturer's instructions.

    Article Title: Food Structure Modulation of Antioxidant Bioaccessibility and Gut Epithelial Protection: The Case of Bread and Pasta Made With Pigmented Wheat
    Article Snippet: Total RNA was extracted from the Caco‐2 pellet using the Total RNA Purification Plus Kit (Norgen Biotek, Thorold, ON, Canada), according to the manufacturer's instructions and quantified (NanoDrop, Thermo Fisher Scientific, Italy). .. RNA (1 μg) was retrotranscribed to cDNA using the PrimeScript RT‐PCR Kit (Takara Bio, Göteborg, Sweden), and quantitative real‐time PCR (Biorad CFX96) was used to perform the gene expression analysis using TB Green Premix Ex Taq (Takara Bio, Göteborg, Sweden). ..

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: Cellular mRNA was extracted using the RNeasy Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. .. Reverse transcription and subsequent qPCR were performed using the One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time; cat. no. RR066A; Takara Bio, Inc.), which includes TB Green as the fluorophore, according to the manufacturer's protocol. .. RT-qPCR was performed using the Thermal Cycler Dice Real Time System II (Takara Bio, Inc.) following the manufacturer's instructions.

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: CD36/ANXA1/TLR4/NF-κB Axis Orchestrates Lipid Metabolism, Chronic Inflammation, and Insulin Resistance in Obese Children.
    Article Snippet: Background: Childhood obesity is tightly linked to dyslipidemia, chronic low-grade inflammation, and insulin resistance (IR).. CD36 has been implicated in metabolic disease, yet its pediatric mechanisms remain unclear.. This study aims to investigate the CD36/ANXA1/TLR4/NF-κB axis as a potential therapeutic target for lipid metabolism and IR in childhood obesity.

    Article Title: Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.
    Article Snippet: .. SYBR Green RT-qPCR was performed using the One step primescript RT-PCR kit (Takara) according to manufacturer’s instructions. ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Gene Expression:

    Article Title: Food Structure Modulation of Antioxidant Bioaccessibility and Gut Epithelial Protection: The Case of Bread and Pasta Made With Pigmented Wheat
    Article Snippet: Total RNA was extracted from the Caco‐2 pellet using the Total RNA Purification Plus Kit (Norgen Biotek, Thorold, ON, Canada), according to the manufacturer's instructions and quantified (NanoDrop, Thermo Fisher Scientific, Italy). .. RNA (1 μg) was retrotranscribed to cDNA using the PrimeScript RT‐PCR Kit (Takara Bio, Göteborg, Sweden), and quantitative real‐time PCR (Biorad CFX96) was used to perform the gene expression analysis using TB Green Premix Ex Taq (Takara Bio, Göteborg, Sweden). ..

    cDNA Synthesis:

    Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism
    Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the PrimeScriptTM RT-PCR Kit (Takara, Tokyo, Japan), while reverse transcription of miRNAs was carried out with the miRNA 1st Strand cDNA Synthesis Kit (Vazyme, Nanjing, China). qPCR was conducted by using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara, Tokyo, Japan). ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Polymerase Chain Reaction:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Amplification:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    One Step RT-PCR:

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
    Article Snippet: Total RNA was extracted from PBMCs using TRIzol reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions. .. The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction. ..

    Quantitative RT-PCR:

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Article Title: Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.
    Article Snippet: .. SYBR Green RT-qPCR was performed using the One step primescript RT-PCR kit (Takara) according to manufacturer’s instructions. ..

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    Synthesized:

    Article Title: CD36/ANXA1/TLR4/NF-κB Axis Orchestrates Lipid Metabolism, Chronic Inflammation, and Insulin Resistance in Obese Children.
    Article Snippet: Background: Childhood obesity is tightly linked to dyslipidemia, chronic low-grade inflammation, and insulin resistance (IR).. CD36 has been implicated in metabolic disease, yet its pediatric mechanisms remain unclear.. This study aims to investigate the CD36/ANXA1/TLR4/NF-κB axis as a potential therapeutic target for lipid metabolism and IR in childhood obesity.

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).

    SYBR Green Assay:

    Article Title: Characterization of the virus-host RNA-RNA interactome across important human pathogenic RNA viruses.
    Article Snippet: .. SYBR Green RT-qPCR was performed using the One step primescript RT-PCR kit (Takara) according to manufacturer’s instructions. ..

    RNA Sequencing:

    Article Title: Molecular basis of copper homeostasis in Trichoderma asperellum Ts93: roles of a peroxidase and Ctr-family transporters.
    Article Snippet: agents, fungi exhibit superior metal chelation capabilities for the removal of heavy metals (Rosa et al. 2024; Vaksmaa et al. 2023).. Consequently, investigating the copper tolerance mechanisms in fungi is essential for developing effective bioremediation strategies.. The genus Trichoderma comprises filamentous fungi renowned for their ecological versatility and biotechnological applications, including their ability to tolerate elevated copper levels (Ramírez-Valdespino & Orrantia-Borunda, 2021).



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