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takara sybr rtpcr kits  (TaKaRa)


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    Structured Review

    TaKaRa takara sybr rtpcr kits
    Takara Sybr Rtpcr Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sybr+primescript+rtpcr+kit/PrimeScript+RT-PCR+Kit/pm40633210-194-46-46
    Average 98 stars, based on 10987 article reviews
    takara sybr rtpcr kits - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The capsid proteins of orthoflavivirus are interchangeable for West Nile virus assembly.
    Article Snippet: .. Total RNAs of WNV-DV2mC-P10-A/B/C-infected cells were extracted, and RT-PCR was then carried out using the PrimeScript One Step RT-PCR Kit (Takara) with primers to amplify the fragments spanning from 5′ UTR to 3′ UTR. ..

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus.
    Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus).

    Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy
    Article Snippet: .. Measurements of host gene expression were performed using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Takara). ..

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    One Step RT-PCR:

    Article Title: The capsid proteins of orthoflavivirus are interchangeable for West Nile virus assembly.
    Article Snippet: .. Total RNAs of WNV-DV2mC-P10-A/B/C-infected cells were extracted, and RT-PCR was then carried out using the PrimeScript One Step RT-PCR Kit (Takara) with primers to amplify the fragments spanning from 5′ UTR to 3′ UTR. ..

    Synthesized:

    Article Title: The Gut-Liver Axis Mediates Liver Injury Induced by Low-Molecular-Weight Polycyclic Aromatic Hydrocarbons Through TLR4-Dependent Inflammation and Bile Acid Dysregulation.
    Article Snippet: Total RNA was extracted from colon and liver tissue samples (0.1 g) using RNAiso Plus reagent (TaKaRa, Kyoto, Japan). .. cDNA was synthesized using the PrimeScript RT kit (TaKaRa, Japan) and amplified using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Japan) on a real-time PCR system (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Amplification:

    Article Title: The Gut-Liver Axis Mediates Liver Injury Induced by Low-Molecular-Weight Polycyclic Aromatic Hydrocarbons Through TLR4-Dependent Inflammation and Bile Acid Dysregulation.
    Article Snippet: Total RNA was extracted from colon and liver tissue samples (0.1 g) using RNAiso Plus reagent (TaKaRa, Kyoto, Japan). .. cDNA was synthesized using the PrimeScript RT kit (TaKaRa, Japan) and amplified using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Japan) on a real-time PCR system (Bio-Rad, Hercules, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: The Gut-Liver Axis Mediates Liver Injury Induced by Low-Molecular-Weight Polycyclic Aromatic Hydrocarbons Through TLR4-Dependent Inflammation and Bile Acid Dysregulation.
    Article Snippet: Total RNA was extracted from colon and liver tissue samples (0.1 g) using RNAiso Plus reagent (TaKaRa, Kyoto, Japan). .. cDNA was synthesized using the PrimeScript RT kit (TaKaRa, Japan) and amplified using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Japan) on a real-time PCR system (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Reverse Transcription:

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Article Title: Decitabine induces cell differentiation and growth inhibition accompanied by upregulation of microRNA-143 and MS4A3 in the myelodysplastic syndromes-derived leukemic cell line MDS-L.
    Article Snippet: RNA was extracted from cells using a RNeasy Mini Kit (Qiagen Sciences, Inc.). .. Total RNA was reverse transcribed to cDNA using a PrimeScript RT‐PCR kit (Takara Bio, Inc.) and TaqMan MicroRNA Reverse Transcription kit (cat. no. 4366597; Thermo Fisher Scientific, Inc.). .. Quantitative PCR was carried out using a TaqMan Fast Advanced Master Mix kit and Step One Plus real‐time PCR systems (Applied Biosystems; Thermo Fisher Scientific, Inc.) with the following conditions: An initial 95 ̊C for 20 sec, followed by 40 cycles of 95 ̊C for 1 sec and 60 ̊C for 20 sec.

    Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus.
    Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus).

    Gene Expression:

    Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy
    Article Snippet: .. Measurements of host gene expression were performed using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Takara). ..

    Concentration Assay:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Spectrophotometry:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    other:

    Article Title: piR-823 and piR-20326 Differentially Regulate Stemness Programs in Ovarian Cancer Stem-like Cells.
    Article Snippet: The stem–loop primers were used for the “PrimeScript Reagent Kit Synthesis System for RT-PCR (Takara Bio USA, Inc., San Jose, CA, USA)” piRNA analysis, and a U6-specific reverse primer was used for the endogenous control.



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