Review



surface β1ar quantitative colocalization analysis  (Nikon)


Bioz Verified Symbol Nikon is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Nikon surface β1ar quantitative colocalization analysis
    Surface β1ar Quantitative Colocalization Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surface+colocalization+analysis/NIS-Elements/pmc03340205-99-11-29
    Average 99 stars, based on 40098 article reviews
    surface β1ar quantitative colocalization analysis - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Confocal:

    Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model.
    Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the NIS Elements software (ver. ..

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Microscopy:

    Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model.
    Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the NIS Elements software (ver. ..

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring.
    Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20.

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Discovery of a Virulent Population of Meloidogyne graminicola Overcoming the Rice Resistance Gene MG1 and Population Genetic Analysis Based on mtCOI Sequences.
    Article Snippet: .. Measurements and photographs were https://doi.org/10.3390/plants15193031 taken with a Nikon ECLIPSE Ni microscope equipped with a Nikon Digital Sight Camera and NIS-Elements BR 4.13.05 software (Nikon, Tokyo, Japan). ..

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Software:

    Article Title: Mechanisms of Hexavalent Chromium-Induced Lung Carcinogenesis: DNA Double-Strand Breaks and DNA Repair Inhibition in a Guinea Pig Model.
    Article Snippet: .. These lung regions were imaged using a Nikon A1 Confocal Microscope Ti with the NIS Elements software (ver. ..

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring.
    Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20.

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Discovery of a Virulent Population of Meloidogyne graminicola Overcoming the Rice Resistance Gene MG1 and Population Genetic Analysis Based on mtCOI Sequences.
    Article Snippet: .. Measurements and photographs were https://doi.org/10.3390/plants15193031 taken with a Nikon ECLIPSE Ni microscope equipped with a Nikon Digital Sight Camera and NIS-Elements BR 4.13.05 software (Nikon, Tokyo, Japan). ..

    Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut
    Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon ECLIPSE Ti2 inverted microscope, NiS‐Elements D, version 5.30.02 software). .. Brightfield images of the gut were overlaid with fluorescent images to accurately demarcate the region of interest before quantification of gut fluorescent intensity (using ImageJ software).

    Fluorescence:

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Article Title: Prenatal microplastic exposure-induced testicular developmental toxicity and its long-term hazards in male rat offspring.
    Article Snippet: The pervasive environmental presence of microplastics (MPs) raises concerns regarding their potential impacts on reproductive health, particularly in the context of prenatal exposure.. This study aimed to establish a polystyrene microplastics (PS-MPs) exposure model in pregnant rats and to investigate the subsequent effects on the growth, hormonal profiles, steroidogenic enzyme expression, and testicular histology of male offspring from birth to adulthood.. Pregnant rats were administered PS-MPs at doses of 1 mg/kg/day (MPs-L) and 10 mg/kg/day (MPs-H) during gestational days 10–20.

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut
    Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon ECLIPSE Ti2 inverted microscope, NiS‐Elements D, version 5.30.02 software). .. Brightfield images of the gut were overlaid with fluorescent images to accurately demarcate the region of interest before quantification of gut fluorescent intensity (using ImageJ software).

    Automated:

    Article Title: Peripheral nerve injury alters metabolic function and neuronal excitability through the Lin28/let-7 pathway
    Article Snippet: .. Image pairs were continually acquired at 340-nm 100-ms excitation and 380-nm 200-ms excitation at 1.5-s intervals using an inverted fluorescence microscope (TE200, Nikon) equipped with a complementary metal-oxide semiconductor camera (Neo, Andor) and an automated filter wheel (Lambda), controlled by the NIS elements software (Nikon), and emission at 340/380 nm was calculated. .. Cells were imaged during continuous perfusion with CIB and then challenged with sequential 30-s applications of 15 mM KCl, capsaicin (10 nM; Sigma-Aldrich), and 50 mM KCl, with 3-min intervening washouts with CIB.

    Imaging:

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Inverted Microscopy:

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Article Title: Expanded characterization of the valproic acid zebrafish larval model of autism spectrum disorder: Insights into dysregulation in the gut
    Article Snippet: Magnesium sulfate (208094, Sigma‐Aldrich, Wisconsin, United States; 33.2 mmol/L) and atropine (A0257, Sigma‐Aldrich, St Louis, United States; 5 mmol/L) were included as positive controls to mimic accelerated and delayed gut motility, respectively. .. Larvae were anesthetized and orientated as described in the NR protocol before live images of gut fluorescence were acquired at 100× magnification (Nikon ECLIPSE Ti2 inverted microscope, NiS‐Elements D, version 5.30.02 software). .. Brightfield images of the gut were overlaid with fluorescent images to accurately demarcate the region of interest before quantification of gut fluorescent intensity (using ImageJ software).

    Analysis:

    Article Title: Sirt3 deficiency impairs AMPK-Acc1 antilipogenic signaling in female adipocytes.
    Article Snippet: 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia 2 Division of Materials Chemistry, Rudjer Boskovic Institute, Zagreb, Croatia 3 Faculty of Science, Department of Biology, University of Zagreb, Zagreb, Croatia 4 Faculty of Biology, Center for Electron Microscopy, University of Belgrade, Belgrade, Serbia Abstract Background Background: Biological sex influences adipose tissue function, while impaired adipogenesis contributes to adipose dysfunction.. Sirtuin 3 (Sirt3), a mitochondrial NAD+-dependent deacetylase involved in oxidative metabolism and stress adaptation, remains insufficiently defined in adipogenic differentiation.. We investigated how Sirt3 and sex shape adipogenic phenotype in mouse embryonic fibroblast (MEF)-derived adipocytes.

    Light Microscopy:

    Article Title: Leptin promotes obesity‑related osteoarthritis progression via CD44/JAK2/STAT3 axis‑mediated chondrocyte apoptosis.
    Article Snippet: .. Following dehydration in increasing ethanol concentrations (70‐100%) and xylene clearing, the slides were mounted with neutral balsam and images were captured under a Nikon Eclipse 80i light microscope (Nikon Corporation,) using NIS‐Elements BR software (version 5.42; Nikon Corporation). ..

    Polymerase Chain Reaction:

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Amplification:

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..

    Expressing:

    Article Title: Trichoderma harzianum M2 Induces Systemic Resistance to Fusarium Crown Rot and Remodels Root Architecture to Promote Phosphorus Uptake in Wheat.
    Article Snippet: .. Putative positive transformants were further verified by PCR amplification of the GFP gene, and GFP fluorescence signal was detected using a Nikon confocal microscope (AX NIS-Elements 5.4, Tokyo, Japan) to confirm GFP expression. ..



    Similar Products

    99
    Oxford Instruments surface surface colocalization analysis
    A-B ) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in in situ fixed, freshly sorted quiescent and 4h in vitro activated MuSCs ( n =48-55 cells from 3 mice in each group and experimental condition). C) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in <t>colocalization.</t> D) Colocalization of PARKIN to TOM20-labeled mitochondria expressed in absolute volume of PARKIN + structures overlapping with mitochondria per cell ( n =25-36 cells from 3 mice in each group and experimental condition). E) Proportion of TOM20-labeled mitochondria labeled with PARKIN, computed using data presented in panel D and total mitochondrial content (Fig. S1A). F) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and PARKIN-labeled structures (red) in each of the experimental conditions examined. The volume of PARKIN overlapping with mitochondria is shown in yellow in the right-end panels, where mitochondria and PARKIN + surfaces have been removed to highlight changes in colocalization. G) Total cellular PARKIN content in the indicated experimental conditions. H) Parkin transcript levels in MuSCs purified from muscles that were fixed in situ in healthy uninjured conditions or at 15, 30, 60, 90 and 120 min after CTX injury. Data is taken from (GSE163856 ). All data are presented as mean ± SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs. I) Changes in the transcript levels of ubiquitin- and receptor-dependent mitophagy in three transcriptomics datasets (GSE70736, GSE55490, GSE47177 , , ) that compared quiescent and in vivo activated MuSCs 36-72h following muscle injury with CTX or BaCl 2 . Each dot represents data from an individual dataset and a specific timepoints. Genes shown where differentially expressed in all datasets with a q value of less than 0.05. Level of statistical significance shown is based on the average q value.
    Surface Surface Colocalization Analysis, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surface+colocalization+analysis/Imaris/bio_rxiv__64898__2026__03__20__712989-193-3-9
    Average 99 stars, based on 1 article reviews
    surface surface colocalization analysis - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Oxford Instruments imaris surface colocalization analysis
    A-B ) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in in situ fixed, freshly sorted quiescent and 4h in vitro activated MuSCs ( n =48-55 cells from 3 mice in each group and experimental condition). C) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in <t>colocalization.</t> D) Colocalization of PARKIN to TOM20-labeled mitochondria expressed in absolute volume of PARKIN + structures overlapping with mitochondria per cell ( n =25-36 cells from 3 mice in each group and experimental condition). E) Proportion of TOM20-labeled mitochondria labeled with PARKIN, computed using data presented in panel D and total mitochondrial content (Fig. S1A). F) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and PARKIN-labeled structures (red) in each of the experimental conditions examined. The volume of PARKIN overlapping with mitochondria is shown in yellow in the right-end panels, where mitochondria and PARKIN + surfaces have been removed to highlight changes in colocalization. G) Total cellular PARKIN content in the indicated experimental conditions. H) Parkin transcript levels in MuSCs purified from muscles that were fixed in situ in healthy uninjured conditions or at 15, 30, 60, 90 and 120 min after CTX injury. Data is taken from (GSE163856 ). All data are presented as mean ± SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs. I) Changes in the transcript levels of ubiquitin- and receptor-dependent mitophagy in three transcriptomics datasets (GSE70736, GSE55490, GSE47177 , , ) that compared quiescent and in vivo activated MuSCs 36-72h following muscle injury with CTX or BaCl 2 . Each dot represents data from an individual dataset and a specific timepoints. Genes shown where differentially expressed in all datasets with a q value of less than 0.05. Level of statistical significance shown is based on the average q value.
    Imaris Surface Colocalization Analysis, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surface+colocalization+analysis/Imaris/pm37537212-456-0-0
    Average 99 stars, based on 1 article reviews
    imaris surface colocalization analysis - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Oxford Instruments surface colocalization analysis
    A-B ) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in in situ fixed, freshly sorted quiescent and 4h in vitro activated MuSCs ( n =48-55 cells from 3 mice in each group and experimental condition). C) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in <t>colocalization.</t> D) Colocalization of PARKIN to TOM20-labeled mitochondria expressed in absolute volume of PARKIN + structures overlapping with mitochondria per cell ( n =25-36 cells from 3 mice in each group and experimental condition). E) Proportion of TOM20-labeled mitochondria labeled with PARKIN, computed using data presented in panel D and total mitochondrial content (Fig. S1A). F) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and PARKIN-labeled structures (red) in each of the experimental conditions examined. The volume of PARKIN overlapping with mitochondria is shown in yellow in the right-end panels, where mitochondria and PARKIN + surfaces have been removed to highlight changes in colocalization. G) Total cellular PARKIN content in the indicated experimental conditions. H) Parkin transcript levels in MuSCs purified from muscles that were fixed in situ in healthy uninjured conditions or at 15, 30, 60, 90 and 120 min after CTX injury. Data is taken from (GSE163856 ). All data are presented as mean ± SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs. I) Changes in the transcript levels of ubiquitin- and receptor-dependent mitophagy in three transcriptomics datasets (GSE70736, GSE55490, GSE47177 , , ) that compared quiescent and in vivo activated MuSCs 36-72h following muscle injury with CTX or BaCl 2 . Each dot represents data from an individual dataset and a specific timepoints. Genes shown where differentially expressed in all datasets with a q value of less than 0.05. Level of statistical significance shown is based on the average q value.
    Surface Colocalization Analysis, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surface+colocalization+analysis/Imaris/pm37537212-456-1-0
    Average 99 stars, based on 1 article reviews
    surface colocalization analysis - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Nikon surface β1ar quantitative colocalization analysis
    A-B ) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in in situ fixed, freshly sorted quiescent and 4h in vitro activated MuSCs ( n =48-55 cells from 3 mice in each group and experimental condition). C) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in <t>colocalization.</t> D) Colocalization of PARKIN to TOM20-labeled mitochondria expressed in absolute volume of PARKIN + structures overlapping with mitochondria per cell ( n =25-36 cells from 3 mice in each group and experimental condition). E) Proportion of TOM20-labeled mitochondria labeled with PARKIN, computed using data presented in panel D and total mitochondrial content (Fig. S1A). F) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and PARKIN-labeled structures (red) in each of the experimental conditions examined. The volume of PARKIN overlapping with mitochondria is shown in yellow in the right-end panels, where mitochondria and PARKIN + surfaces have been removed to highlight changes in colocalization. G) Total cellular PARKIN content in the indicated experimental conditions. H) Parkin transcript levels in MuSCs purified from muscles that were fixed in situ in healthy uninjured conditions or at 15, 30, 60, 90 and 120 min after CTX injury. Data is taken from (GSE163856 ). All data are presented as mean ± SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs. I) Changes in the transcript levels of ubiquitin- and receptor-dependent mitophagy in three transcriptomics datasets (GSE70736, GSE55490, GSE47177 , , ) that compared quiescent and in vivo activated MuSCs 36-72h following muscle injury with CTX or BaCl 2 . Each dot represents data from an individual dataset and a specific timepoints. Genes shown where differentially expressed in all datasets with a q value of less than 0.05. Level of statistical significance shown is based on the average q value.
    Surface β1ar Quantitative Colocalization Analysis, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surface+colocalization+analysis/NIS-Elements/pmc03340205-99-11-29
    Average 99 stars, based on 1 article reviews
    surface β1ar quantitative colocalization analysis - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    A-B ) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in in situ fixed, freshly sorted quiescent and 4h in vitro activated MuSCs ( n =48-55 cells from 3 mice in each group and experimental condition). C) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in colocalization. D) Colocalization of PARKIN to TOM20-labeled mitochondria expressed in absolute volume of PARKIN + structures overlapping with mitochondria per cell ( n =25-36 cells from 3 mice in each group and experimental condition). E) Proportion of TOM20-labeled mitochondria labeled with PARKIN, computed using data presented in panel D and total mitochondrial content (Fig. S1A). F) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and PARKIN-labeled structures (red) in each of the experimental conditions examined. The volume of PARKIN overlapping with mitochondria is shown in yellow in the right-end panels, where mitochondria and PARKIN + surfaces have been removed to highlight changes in colocalization. G) Total cellular PARKIN content in the indicated experimental conditions. H) Parkin transcript levels in MuSCs purified from muscles that were fixed in situ in healthy uninjured conditions or at 15, 30, 60, 90 and 120 min after CTX injury. Data is taken from (GSE163856 ). All data are presented as mean ± SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs. I) Changes in the transcript levels of ubiquitin- and receptor-dependent mitophagy in three transcriptomics datasets (GSE70736, GSE55490, GSE47177 , , ) that compared quiescent and in vivo activated MuSCs 36-72h following muscle injury with CTX or BaCl 2 . Each dot represents data from an individual dataset and a specific timepoints. Genes shown where differentially expressed in all datasets with a q value of less than 0.05. Level of statistical significance shown is based on the average q value.

    Journal: bioRxiv

    Article Title: Loss of Parkin Disrupts Nuclear and Mitochondrial Programs Required for Muscle Regeneration

    doi: 10.64898/2026.03.20.712989

    Figure Lengend Snippet: A-B ) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in in situ fixed, freshly sorted quiescent and 4h in vitro activated MuSCs ( n =48-55 cells from 3 mice in each group and experimental condition). C) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in colocalization. D) Colocalization of PARKIN to TOM20-labeled mitochondria expressed in absolute volume of PARKIN + structures overlapping with mitochondria per cell ( n =25-36 cells from 3 mice in each group and experimental condition). E) Proportion of TOM20-labeled mitochondria labeled with PARKIN, computed using data presented in panel D and total mitochondrial content (Fig. S1A). F) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and PARKIN-labeled structures (red) in each of the experimental conditions examined. The volume of PARKIN overlapping with mitochondria is shown in yellow in the right-end panels, where mitochondria and PARKIN + surfaces have been removed to highlight changes in colocalization. G) Total cellular PARKIN content in the indicated experimental conditions. H) Parkin transcript levels in MuSCs purified from muscles that were fixed in situ in healthy uninjured conditions or at 15, 30, 60, 90 and 120 min after CTX injury. Data is taken from (GSE163856 ). All data are presented as mean ± SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs. I) Changes in the transcript levels of ubiquitin- and receptor-dependent mitophagy in three transcriptomics datasets (GSE70736, GSE55490, GSE47177 , , ) that compared quiescent and in vivo activated MuSCs 36-72h following muscle injury with CTX or BaCl 2 . Each dot represents data from an individual dataset and a specific timepoints. Genes shown where differentially expressed in all datasets with a q value of less than 0.05. Level of statistical significance shown is based on the average q value.

    Article Snippet: Volume reconstruction and surface/surface colocalization analysis was performed in Imaris using fixed settings.

    Techniques: In Situ, In Vitro, Labeling, Purification, Muscles, Two Tailed Test, Ubiquitin Proteomics, Transcriptomics, In Vivo

    A-C) Parkin transcript and protein levels in FACS-purified MuSCs from MuSC Park2 +/+ and MuSC Park2 -/- mice 1 week after the last tamoxifen treatment ( n =4 mice per group). Protein abundance was quantified as the total volume of PARKIN + structures (B) in cells labeled with antibodies against PARKIN and TOM20 ( n =11-70 cells from 2-4 mice per group,) (C). D-E) transcript abundance expressed in Transcript Per Million (TPM) for genes involved in Parkin- and Receptor-mediated mitophagy ( n =3 mice per group). F-G) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in freshly sorted quiescent and 4h in vitro activated MuSCs ( n =41-52 cells from 3 mice in each group). H) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in colocalization. All data are presented as mean ±SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs.

    Journal: bioRxiv

    Article Title: Loss of Parkin Disrupts Nuclear and Mitochondrial Programs Required for Muscle Regeneration

    doi: 10.64898/2026.03.20.712989

    Figure Lengend Snippet: A-C) Parkin transcript and protein levels in FACS-purified MuSCs from MuSC Park2 +/+ and MuSC Park2 -/- mice 1 week after the last tamoxifen treatment ( n =4 mice per group). Protein abundance was quantified as the total volume of PARKIN + structures (B) in cells labeled with antibodies against PARKIN and TOM20 ( n =11-70 cells from 2-4 mice per group,) (C). D-E) transcript abundance expressed in Transcript Per Million (TPM) for genes involved in Parkin- and Receptor-mediated mitophagy ( n =3 mice per group). F-G) Volume of mitochondria colocalized to autophagosomes expressed in absolute value or relative to total mitochondrial volume in freshly sorted quiescent and 4h in vitro activated MuSCs ( n =41-52 cells from 3 mice in each group). H) Confocal image and 3D reconstruction of TOM20-labeled mitochondria (green) and LC3-labeled autophagosomes (red) in each of the experimental conditions examined. The volume of mitochondria overlapping with autophagosomes is shown in yellow in the right-end panels, where mitochondria and autophagosomes surfaces have been removed to highlight changes in colocalization. All data are presented as mean ±SEM. ns: not significant, *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0001 on unpaired two-tailed t tests or one-way ANOVAs.

    Article Snippet: Volume reconstruction and surface/surface colocalization analysis was performed in Imaris using fixed settings.

    Techniques: Purification, Quantitative Proteomics, Labeling, In Vitro, Two Tailed Test