superscript-ii kit (Thermo Fisher)

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Thermo Fisher superscript-ii kit
Superscript Ii Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript-ii+kit/superscript+ii+kit/pm40646276-339-14-16
Average 90 stars, based on 1 article reviews

superscript-ii kit - by Bioz Stars, 2026-11

90/100 stars

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Quantitative RT-PCR:

Article Title: Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR–Cas9 Genome Editing
Article Snippet: Sanger sequencing was conducted with the ABI Prism 3500 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). .. Complementary DNA was transcribed from 1 μg of RNA with the SuperScript II Kit (Thermo Fisher Scientific, Waltham, MA, USA). qRT-PCR was performed using reaction mixtures containing 5 μL of LightCycler 480 SYBR Green I Master Reagent (Roche, Basel, Switzerland), 0.25 μL of 25 μM forward and reverse primers (Biomers, Ulm, Germany), 2 μL of water, and 2.5 μL of 1:10 diluted complementary DNA. lists the primer sequences. .. The PCR was conducted on a LightCycler 480 Instrument II system (Roche, Basel, Switzerland).

Article Title: Investigation on ABCC6-Deficient Human Hepatocytes Generated by CRISPR-Cas9 Genome Editing.
Article Snippet: Sanger sequencing was conducted with the ABI Prism 3500 Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). .. Complementary DNA was transcribed from 1 μg of RNA with the SuperScript II Kit (Thermo Fisher Scientific, Waltham, MA, USA). qRT-PCR was performed using reaction mixtures containing 5 μL of LightCycler 480 SYBR Green I Master Reagent (Roche, Basel, Switzerland), 0.25 μL of 25 μM forward and reverse primers (Biomers, Ulm, Germany), 2 μL of water, and 2.5 μL of 1:10 diluted complementary DNA. ..

SYBR Green Assay:

other:

Article Title: TRIM33 loss reduces androgen receptor transcriptional output and H2BK120 ubiquitination.
Article Snippet: dTRIM24 (Medchemexpress, HY-111519) was dissolved in DMSO and used in the indicated concentrations.

Reverse Transcription:

Article Title: Mitochondrial Antiviral Signaling Protein Activation by Retinoic Acid-Inducible Gene I Agonist Triggers Potent Antiviral Defense in Umbilical Cord Mesenchymal Stromal Cells Without Compromising Mitochondrial Function.
Article Snippet: Total RNA was extracted using TRIzol (ThermoFisher Scientific) from cultured MSCs according to the manufacturer’s instructions. .. RNA (500 ng), as described previously [5], was reverse transcribed by using a superscript II kit (Invitrogen, Waltham, MA, USA), and qPCR was performed at Stratagene Mx3000P (Agilent Technologies, Santa Clara, CA, USA) with specific primers (Table 1) or Taqman probes (ThermoFisher Scientific) (Table 2). ..

Article Title: Mitochondrial Antiviral Signaling Protein Activation by Retinoic Acid-Inducible Gene I Agonist Triggers Potent Antiviral Defense in Umbilical Cord Mesenchymal Stromal Cells Without Compromising Mitochondrial Function
Article Snippet: Total RNA was extracted using TRIzol (ThermoFisher Scientific) from cultured MSCs according to the manufacturer’s instructions. .. RNA (500 ng), as described previously [ ], was reverse transcribed by using a superscript II kit (Invitrogen, Waltham, MA, USA), and qPCR was performed at Stratagene Mx3000P (Agilent Technologies, Santa Clara, CA, USA) with specific primers ( ) or Taqman probes (ThermoFisher Scientific) ( ). ..

Article Title: The full transcription map of cottontail rabbit papillomavirus in tumor tissues
Article Snippet: To remove contaminating genomic DNA, total RNA was treated with a TURBO DNA-free Kit (Ambion). .. Reverse transcription (RT) was performed with the SuperScript II kit (Thermo Fisher Scientific). .. Amplification of reversed transcribed cDNA was performed by PCR using the AmpliTaq Polymerase Kit (Thermo Fisher Scientific) according to the manufacturer’s protocols.

Article Title: Tumor-secreted TNF induces NCoA3 upregulation in breast cancer-associated adipocytes via TNFR1/NF-κB signaling.
Article Snippet: In breast cancer, adipocytes are the predominant cell type in the microenvironment, and the continuous communication between these tissues alters the adipose phenotype.. However, molecular mechanisms promoting these changes are still poorly understood.. Previously, we demonstrated that NCoA3 expression is increased in adipose tissue adjacent to breast cancer and that this increase is associated with an inflammatory profile.

Real-time Polymerase Chain Reaction:

Article Title: Mitochondrial Antiviral Signaling Protein Activation by Retinoic Acid-Inducible Gene I Agonist Triggers Potent Antiviral Defense in Umbilical Cord Mesenchymal Stromal Cells Without Compromising Mitochondrial Function
Article Snippet: Total RNA was extracted using TRIzol (ThermoFisher Scientific) from cultured MSCs according to the manufacturer’s instructions. .. RNA (500 ng), as described previously [ ], was reverse transcribed by using a superscript II kit (Invitrogen, Waltham, MA, USA), and qPCR was performed at Stratagene Mx3000P (Agilent Technologies, Santa Clara, CA, USA) with specific primers ( ) or Taqman probes (ThermoFisher Scientific) ( ). ..

Synthesized:

Article Title: Efficacy and Fate of RNA Interference Molecules in the Green Pea Aphid, Acyrthosiphon pisum.
Article Snippet: RNA interference (RNAi) is a promising technology for controlling insect pests of agriculture.. This technology is mediated through the application of double‐stranded RNAs (dsRNAs), which are processed within the insect cells into small interfering RNAs (siRNAs).. These molecules then target and reduce the expression of the insect‐specific genes that can kill or reduce the performance of the pest.

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