lunascript rt superscript kit (New England Biolabs)

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New England Biolabs lunascript rt superscript kit
Lunascript Rt Superscript Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+rt+kit/LunaScript+RT+SuperMix+Kit/pm41339336-300-13-17
Average 99 stars, based on 1829 article reviews

lunascript rt superscript kit - by Bioz Stars, 2026-09

99/100 stars

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Synthesized:

Article Title: Cost-effective strategies for long-term bovine trophectoderm cell culture.
Article Snippet: RNA concentration and purity were assessed using a NanoDrop spectrophotometer. .. Complementary DNA (cDNA) was synthesized from total RNA using the LunaScript SuperMix Kit (New England Biolabs). .. Quantitative PCR reactions were performed using SYBR Green qPCR Master Mix (GlpBio) in a CFX96 Real-Time PCR Detection System (Bio-Rad).

Article Title: Perturbation of BRD4 and p300 activity suppresses super enhancer-driven KLF6 expression in renal carcinoma
Article Snippet: Total RNA was extracted from ccRCC cells using TRIzol ® reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The yield and purity of total RNA were quantified using the NanoDropTM 2000c Spectrophotometer (Thermo Fisher Scientific, Inc.). cDNA was synthesized from total RNA using a LunaScript RT SuperMix kit (New England BioLabs, Inc.) as follows: 25°C for 2 min, 55°C for 10 min and 95°C for 1 min. RT-qPCR was performed using TaqMan chemistry with pre-designed TaqMan probes (Thermo Fisher Scientific, Inc.; cat. nos. .. KLF6 Hs00810569_m1, β-actin Hs01060665_g1) and Luna Universal Probe qPCR Master Mix (New England BioLabs, Inc.). qPCR reactions were run on the 7500 Fast Real-Time System (Applied Biosystems; Thermo Fisher Scientific, Inc.) with the following cycling conditions: 95°C for 1 min for initial denaturation, followed by 40 cycles of 95°C for 15 s and 60°C for 30 s. β-actin served as the reference gene for normalization and gene expression fold-changes were calculated using the 2 −ΔΔCq method ( ).

cDNA Synthesis:

Article Title: An engineered insulin analog with dual insulin and IGF-1 receptor agonism and distinct signaling
Article Snippet: The aqueous phase was transferred to new tubes, 100% ethanol in ratio 1:1 was added, and subsequently, the Direct-zol RNA Miniprep Kit (Zymo Research) was used according to the manufacturer’s instructions. .. For subsequent cDNA synthesis, LunaScript RT SuperMix Kit (New England Biolabs) was used according to the manufacturer’s instructions. .. The qPCR was performed using LightCycler 480 SYBR Green I Master (Roche) according to the manufacturer’s instructions on LightCycler 480 II (Roche).

Reverse Transcription:

Article Title: Single-cell transcriptomics highlights macrophage-driven regulation of EndMT and repair in injured muscle.
Article Snippet: Total RNA was quantified by measuring the absorbance at 260 nm in a Nanodrop (Thermofisher Scientific) and RNA purity was evaluated by the ratio of absorbance at 260 and 280 nm. .. RNA samples were reverse transcribed to cDNA with the LunaScript RT SuperMix Kit (E3010L) from New England Biolabs, using the Thermocycler 2027 (Applied Biosystems). .. Real-time rt-qPCR was carried out using the Luna Universal qPCR Master Mix (M3003X, New England Biolabs) and specific primers (Table II) in a QuantStudioTM 7 Flex Real-Time PCR System (Applied Biosystems).

Article Title: Genomic epidemiology and phylogeographic reconstruction of West Nile virus 2 in Italy from 2011 to 2023
Article Snippet: RNA from all the samples was manually extracted from bird homogenates (see Supplementary Material) and mosquito pools by using QIAMP viral RNA mini-Kit (Qiagen GmbH, Germany), according to the manufacturer's instructions. .. After the extraction, RNA was quantified on Infinite M200 Pro instrument with NanoQuant plate (Tecan, Trading AG, Switzerland) and only the RNA of positive WNV-2 samples has been reverse-transcribed to cDNA using the LunaScript® RT SuperMix Kit (New England Biolabs, Ipswich, MA) and amplified by two home-made protocol using primer pools generating 400 bp amplicons or specific primers, when the Ct (cycle threshold) value of the qPCR previously performed was ≤33. ..

Article Title: Cocirculation of endemic and recently introduced West Nile Virus lineage 1 clades in Southern Spain
Article Snippet: .. RNA extracted from WNV positive samples was reverse transcribed using LunaScript® RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). .. Multiplex PCR was carried out using Q5® Hot Start High-Fidelity (New England Biolab) protocol, utilizing a panel of 41 primer pairs previously validated for WNV genome amplification , .

Extraction:

Amplification:

Real-time Polymerase Chain Reaction:

Article Title: Neuronal regulation of infection recovery prevents pathogenic stress and tissue damage
Article Snippet: .. Five hundred ng of RNA was used to generate cDNA in a 20-μL reaction using the LunaScript RT super mix kit (New England Biolab, Catalog No. E3010). qRT-PCR was performed using the prescribed method for New England Biolab’s Luna Universal qPCR Master Mix on an Applied Biosystems StepOnePlus real-time PCR machine. ..

Quantitative RT-PCR:

Spectrophotometry:

Isolation:

Article Title: Differential gene expression drives cell-cycle-dependent transition from monopolar to bipolar growth in Schizosaccharomyces pombe.
Article Snippet: 260 261 RNA isolation, cDNA synthesis, and qPCR gene expression analysis of wild type, 262 cdc10-129, and cdc25-22 mutant cells 263 mRNA was extracted from wildtype and cdc10-129 mutant strains grown at 25°C, 264 36°C for 4 hours and then shifted to 25°C for 150 minutes, using Qiagen RNAeasy Mini 265 kit (Cat. No. 74104). .. The isolated mRNA was then used to synthesize stable cDNA 266 using NEB LunaScript RT SuperMix Kit (E3010). .. Real-time quantitative PCR was 267 performed using the NEB Luna Universal One-Step RT-qPCR kit.

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