superscript ii rnase h reverse transcriptase kit (Thermo Fisher)
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superscript ii rnase h reverse transcriptase kit
Superscript Ii Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+rnase+h+reverse+transcriptase+kit/pmc11555926-77-0-9
Average 90 stars, based on 1 article reviews
Superscript Ii Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+rnase+h+reverse+transcriptase+kit/pmc11555926-77-0-9
Average 90 stars, based on 1 article reviews
superscript ii rnase h reverse transcriptase kit - by Bioz Stars,
2026-10
90/100 stars
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Reverse Transcription:Article Title: Development of a high-throughput 3D culture microfluidic platform for multi-parameter phenotypic and omics profiling of patient-derived organoids Article Snippet: .. Reverse Transcription (RT) was performed in a total volume of 5 μL of RT master mix (0,5 μL dNTP mix (Life Technologies, 18427-013), 0,05 μL Template-switching oligo (TSO; 5-AAGCAGTGGTATCAACGCAGAGTACATrGrG+G-3; IDT) 0,03 μL MgCl 2 (Ambion, cat. no. AM9530G), 1 μL Betaine (Sigma-Aldrich, B0300-1VL), 1 μL Superscript II first-strand buffer (Life Technologies, 18064071), 0,25 μL DTT (Life Technologies, 18064071), 0,25 μL Article Title: The impact of androgen-induced translation in modulating androgen receptor activity Article Snippet: According to the manufacturer's instructions, total RNA was isolated using the DIRECT-ZOL RNA MINIPREP (Cat# R2052, Zymo Research, Freiburg, Germany). .. Article Title: Effects of Nosema ceranae and Lotmaria passim infections on honey bee foraging behaviour and physiology. Article Snippet: Following extraction, the purified RNA pellets were resuspended in nuclease-free water as per the manufacturer’s protocol and then quantity and quality checked using a BioTek Epoch spectrophotometer and Take3 microvolume plate (Agilent, California, USA). .. Following quantification, 400 ng of total RNA were aliquoted for DNA degradation using DNAse I (RNAse-free) (Cat# AM2224, Ambion, Life Technologies, ThermoFisher Scientific, Ontario, Canada), followed by first strand cDNA synthesis using SuperScript II Reverse Transcriptase and Article Title: An interferon-stimulated long non-coding RNA USP30-AS1 as an immune modulator in influenza A virus infection Article Snippet: RNA samples from cells were extracted using RNeasy Mini Kit (74104, Qiagen) as instructed by the manufacturer. .. For detection of host gene expression, RNA was reverse transcribed using Article Title: The N 6 -methyladenosine Epitranscriptomic Landscape of Lung Adenocarcinoma Article Snippet: Total RNAs were purified with RNeasy Mini Kit (Cat. #74106; QIAGEN) or RNAprep Pure Cell/Bacteria Kit (Cat. #4992235/DP430, TIANGEN, China). .. Approximately 5 μg of total RNA was used to deplete rRNA using Ribo-Zero Gold rRNA Removal Kit (Cat. #MRZG12324, Illumina). rRNA-depleted RNAs were fragmented using NEBNext Magnesium RNA Fragmentation Module (Cat. #E6150S, NEB) and then reverse-transcribed by cDNA Synthesis:Article Title: The impact of androgen-induced translation in modulating androgen receptor activity Article Snippet: According to the manufacturer's instructions, total RNA was isolated using the DIRECT-ZOL RNA MINIPREP (Cat# R2052, Zymo Research, Freiburg, Germany). .. Article Title: Effects of Nosema ceranae and Lotmaria passim infections on honey bee foraging behaviour and physiology. Article Snippet: Following extraction, the purified RNA pellets were resuspended in nuclease-free water as per the manufacturer’s protocol and then quantity and quality checked using a BioTek Epoch spectrophotometer and Take3 microvolume plate (Agilent, California, USA). .. Following quantification, 400 ng of total RNA were aliquoted for DNA degradation using DNAse I (RNAse-free) (Cat# AM2224, Ambion, Life Technologies, ThermoFisher Scientific, Ontario, Canada), followed by first strand cDNA synthesis using SuperScript II Reverse Transcriptase and Real-time Polymerase Chain Reaction:Article Title: The impact of androgen-induced translation in modulating androgen receptor activity Article Snippet: According to the manufacturer's instructions, total RNA was isolated using the DIRECT-ZOL RNA MINIPREP (Cat# R2052, Zymo Research, Freiburg, Germany). .. other:Article Title: LGR4 promotes proliferation and homing via activation of the NF-κB signaling pathway in multiple myeloma Article Snippet: Total RNA was reverse transcribed using the Article Title: An environment-responsive SCLA-type GRAS protein controls reproductive strategy in Marchantia polymorpha Article Snippet: DNA contamination was removed with Invitrogen TURBOTM DNase treatment (Catalog number: AM1907) treatment, in accordance with the manufacturer’s instructions. cDNA was synthesised using Gene Expression:Article Title: An interferon-stimulated long non-coding RNA USP30-AS1 as an immune modulator in influenza A virus infection Article Snippet: RNA samples from cells were extracted using RNeasy Mini Kit (74104, Qiagen) as instructed by the manufacturer. .. For detection of host gene expression, RNA was reverse transcribed using |