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superscript iii rnase h-reverse transcriptase kit  (Toyobo)


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    Structured Review

    Toyobo superscript iii rnase h-reverse transcriptase kit
    Superscript Iii Rnase H Reverse Transcriptase Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+rnase+h+reverse+transcriptase+kit/superscript+iii+reverse+transcriptase+kit/pm39561558-87-16-22
    Average 90 stars, based on 1 article reviews
    superscript iii rnase h-reverse transcriptase kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Transcriptome analysis of jejunal mucosal tissue in breeder hens exposed to acute heat stress.
    Article Snippet: For cDNA synthesis, one microgram of RNA from each RNA pool was individually reversetranscribed using the SuperScript III RNAse H-Reverse transcriptase kit (Toyobo, Osaka, Japan) with random primers (Promega, Madison, WI, USA), following the manufacturer’s protocol.

    Article Title: Transcriptome analysis of jejunal mucosal tissue in breeder hens exposed to acute heat stress
    Article Snippet: For cDNA synthesis, one microgram of RNA from each RNA pool was individually reverse-transcribed using the SuperScript III RNAse H-Reverse transcriptase kit (Toyobo, Osaka, Japan) with random primers (Promega, Madison, WI, USA), following the manufacturer's protocol.

    Article Title: FGF9 is required for Purkinje cell development and function in the cerebellum
    Article Snippet: Reverse transcription was performed using the Super-Script III Reverse Transcriptase kit (TOYOBO).

    Concentration Assay:

    Article Title: Knockdown of long non-coding RNA PVT1 inhibits the proliferation of Raji cells through cell cycle regulation
    Article Snippet: Total RNA from the Raji cells of post-transfection was isolated using a TRIzol ® total RNA isolation system (Invitrogen; Thermo Fisher Scientific, Inc.). .. RNA purity and concentration were measured using a spectrophotometer, and RNA was reverse transcribed into first-strand cDNA using random hexamer primers and the reverse transcriptase Superscript II kit (Toyobo Life Science, Osaka, Japan), according to the manufacturer's protocol. .. The 2 −ΔCt method ( ) was used to analyse the relative changes in gene expression in RT-qPCR experiments with SYBR Green (Toyobo Life Science, Japan).

    Spectrophotometry:

    Article Title: Knockdown of long non-coding RNA PVT1 inhibits the proliferation of Raji cells through cell cycle regulation
    Article Snippet: Total RNA from the Raji cells of post-transfection was isolated using a TRIzol ® total RNA isolation system (Invitrogen; Thermo Fisher Scientific, Inc.). .. RNA purity and concentration were measured using a spectrophotometer, and RNA was reverse transcribed into first-strand cDNA using random hexamer primers and the reverse transcriptase Superscript II kit (Toyobo Life Science, Osaka, Japan), according to the manufacturer's protocol. .. The 2 −ΔCt method ( ) was used to analyse the relative changes in gene expression in RT-qPCR experiments with SYBR Green (Toyobo Life Science, Japan).

    Reverse Transcription:

    Article Title: Knockdown of long non-coding RNA PVT1 inhibits the proliferation of Raji cells through cell cycle regulation
    Article Snippet: Total RNA from the Raji cells of post-transfection was isolated using a TRIzol ® total RNA isolation system (Invitrogen; Thermo Fisher Scientific, Inc.). .. RNA purity and concentration were measured using a spectrophotometer, and RNA was reverse transcribed into first-strand cDNA using random hexamer primers and the reverse transcriptase Superscript II kit (Toyobo Life Science, Osaka, Japan), according to the manufacturer's protocol. .. The 2 −ΔCt method ( ) was used to analyse the relative changes in gene expression in RT-qPCR experiments with SYBR Green (Toyobo Life Science, Japan).

    Article Title: ADAMTS13 Improves Hepatic Platelet Accumulation in Pyrrolizidine Alkaloids-induced Liver Injury
    Article Snippet: Plasma ADAMTS13 levels were measured using a Human ADAMTS13 Quantikine ELISA kit (DADT130; R&D Systems, Minneapolis, MN, USA) per the manufacturer’s instructions.23 .. Total RNA was extracted from mouse liver tissues using a Total RNA Kit (DP419; Tiangen, Beijing, China) and synthesized into cDNA using the Superscript II reverse transcriptase kit (TOYOBO, Tokyo, Japan). .. Quantitative PCR amplification was conducted using a Roche LightCycler® 480 II Real-time PCR system and SYBR Green (Tiangen).

    Article Title: ADAMTS13 Improves Hepatic Platelet Accumulation in Pyrrolizidine Alkaloids-induced Liver Injury
    Article Snippet: Plasma ADAMTS13 levels were measured using a Human ADAMTS13 Quantikine ELISA kit (DADT130; R&D Systems, Minneapolis, MN, USA) per the manufacturer’s instructions. .. Total RNA was extracted from mouse liver tissues using a Total RNA Kit (DP419; Tiangen, Beijing, China) and synthesized into cDNA using the Superscript II reverse transcriptase kit (TOYOBO, Tokyo, Japan). .. Quantitative PCR amplification was conducted using a Roche LightCycler® 480 II Real-time PCR system and SYBR Green (Tiangen).

    Article Title: Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
    Article Snippet: Total RNA from cells was extracted using TRIzol Reagent (Invitrogen). .. Then, the extracted RNA was reverse-transcribed into cDNA using the SuperScript TM III First-Strand kit (TOYOBO Co., Ltd., Japan). .. Subsequently, real-time qPCR was conducted using the SYBR Green PCR Master Mix (Life Technologies, USA) and Stratagene Mx3005P PCR System (Agilent Technologies, USA).

    Article Title: OsGPAT3 Plays a Critical Role in Anther Wall Programmed Cell Death and Pollen Development in Rice
    Article Snippet: Total RNA was extracted using the TIANGEN RNAprep Pure Plant Kit as described by the supplier. .. RNA was then reverse-transcribed (RT) from DNase I-treated RNA using Oligo-dT (18) primers in a 20-μL reaction using a SuperScript III Reverse Transcriptase Kit (TOYOBO, Japan). .. For qPCR, first-strand complementary DNA (cDNA) was diluted three times and then 3 μL of the RT products were used as the template of every PCR reaction using SYBR Premix Ex Taq II (TaKaRa) according to the manufacturer’s instructions.

    Random Hexamer:

    Article Title: Knockdown of long non-coding RNA PVT1 inhibits the proliferation of Raji cells through cell cycle regulation
    Article Snippet: Total RNA from the Raji cells of post-transfection was isolated using a TRIzol ® total RNA isolation system (Invitrogen; Thermo Fisher Scientific, Inc.). .. RNA purity and concentration were measured using a spectrophotometer, and RNA was reverse transcribed into first-strand cDNA using random hexamer primers and the reverse transcriptase Superscript II kit (Toyobo Life Science, Osaka, Japan), according to the manufacturer's protocol. .. The 2 −ΔCt method ( ) was used to analyse the relative changes in gene expression in RT-qPCR experiments with SYBR Green (Toyobo Life Science, Japan).

    Synthesized:

    Article Title: ADAMTS13 Improves Hepatic Platelet Accumulation in Pyrrolizidine Alkaloids-induced Liver Injury
    Article Snippet: Plasma ADAMTS13 levels were measured using a Human ADAMTS13 Quantikine ELISA kit (DADT130; R&D Systems, Minneapolis, MN, USA) per the manufacturer’s instructions.23 .. Total RNA was extracted from mouse liver tissues using a Total RNA Kit (DP419; Tiangen, Beijing, China) and synthesized into cDNA using the Superscript II reverse transcriptase kit (TOYOBO, Tokyo, Japan). .. Quantitative PCR amplification was conducted using a Roche LightCycler® 480 II Real-time PCR system and SYBR Green (Tiangen).

    Article Title: ADAMTS13 Improves Hepatic Platelet Accumulation in Pyrrolizidine Alkaloids-induced Liver Injury
    Article Snippet: Plasma ADAMTS13 levels were measured using a Human ADAMTS13 Quantikine ELISA kit (DADT130; R&D Systems, Minneapolis, MN, USA) per the manufacturer’s instructions. .. Total RNA was extracted from mouse liver tissues using a Total RNA Kit (DP419; Tiangen, Beijing, China) and synthesized into cDNA using the Superscript II reverse transcriptase kit (TOYOBO, Tokyo, Japan). .. Quantitative PCR amplification was conducted using a Roche LightCycler® 480 II Real-time PCR system and SYBR Green (Tiangen).



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