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superscript tm iv reverse transcriptase 18090200  (Thermo Fisher)


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    Thermo Fisher superscript tm iv reverse transcriptase 18090200
    Superscript Tm Iv Reverse Transcriptase 18090200, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+tm+reverse+transcriptase/superscript+iv+reverse+transcriptase+18090200/pm39820458-318-0-5
    Average 90 stars, based on 1 article reviews
    superscript tm iv reverse transcriptase 18090200 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Uncovering the whole genome silencers of human cells via Ss-STARR-seq
    Article Snippet: Subsequently, enrich mRNA using Dynabeads Oligo (dT)25 (Thermo Fisher Scientific, 61002). .. SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific; 18090200) and 2 M specific primers (Supplementary Data ) were used for reverse transcription of the enriched mRNA to generate a cDNA product, and then AmbionTM RNase H was used to remove the residual RNA in the cDNA product. .. After purification of the cDNA product, the HiFi high fidelity enzyme Ready Mix (Roche, KK2601) for PCR amplification (Supplementary Data , 98 °C 45 s; after 20 cycles of amplification at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 sec, the products were recovered by 2% agarose gel electrophoresis for 40 min (120 V, 130 mA) to construct the output library for high-throughput sequencing.

    Article Title: SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis
    Article Snippet: .. 120 ng of RNA from T. b. gambiense ELIANE strain for TgSGP or 40 ng of RNA from T. b. rhodesiense EATRO strain for SRA RNA production were retro-transcribed using pT19 oligonucleotide and SuperScript IV Reverse Transcriptase (Thermo Fisher; 200 U/ul 18090200) following standard protocols. cDNAs were purified with Ampure XP (A63880) following manufacturer instructions and eluted in 30 μL of nuclease free water. ..


    Article Title: M-CDC: Magnetic pull-down-assisted colorimetric method based on the CRISPR/Cas12a system.
    Article Snippet: The construction of a rapid, simple, and specific nucleic acid detection platform is of great significance to the control of the large-scale spread of infectious diseases.. We have recently established a magnetic pull-downassisted colorimetric method based on the CRISPR/Cas12a system (termed M− CDC), which effectively integrates the advantages of CRISPR/Cas12a, magnetic beads-based separation, and AuNP bioprobe to provide a simple and specific biosensing platform for nucleic acid assay.. The M− CDC method is compatible with point-ofcare testing and enables the detection of nucleic acid samples in less than an hour without relying on expensive and complex instruments.

    Article Title: Chemically Modified mocRNAs for Highly Efficient Protein Expression in Mammalian Cells.
    Article Snippet: RNA was then quantified using Nanodrop prior to storage at −80 °C. .. Reverse transcription of total RNA was performed using SuperScript IV Reverse Transcriptase (ThermoFisher Scientific: 18090200). .. Then, 500 ng of total RNA was mixed with 1 μL of Random Primer Mix (NEB: S1330S) and brought up to a total volume of 13 μL.

    Article Title: Uncovering the whole genome silencers of human cells via Ss-STARR-seq.
    Article Snippet: Subsequently, enrichmRNA using Dynabeads Oligo (dT)25 (Thermo Fisher Scientific, 61002). .. SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific; 18090200) and 2M specific primers (Supplementary Data 1) were used for reverse transcription of the enriched mRNA to generate a cDNAproduct, and thenAmbionTMRNaseHwasused to remove the residual RNA in the cDNA product. .. After purification of the cDNA product, the HiFi high fidelity enzyme Ready Mix (Roche, KK2601) for PCR amplification (Supplementary Data 1, 98 °C 45 s; after 20 cycles of amplification at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 sec, the products were recovered by 2% agarose gel electrophoresis for 40min (120 V, 130mA) to construct the output library for high-throughput sequencing.

    Article Title: Multiplex generation and single-cell analysis of structural variants in mammalian genomes
    Article Snippet: .. Reverse transcription was performed using the SuperScript IV Reverse Transcriptase (Thermo 18090200) with 0.5 uL 100 μM RT primer that contains an 8-bp degenerate 3′ end (oSP1012). ..

    Article Title: Caspase-1 activates gasdermin A in non-mammals
    Article Snippet: The tissue samples were stored in RNAlater RNA Stabilization Solution (AM7020, Thermo Fisher, Waltham, MA, USA) at −80°C if the experiments were not processed immediately. .. Approximately 20 μg tissue samples were harvested for RNA extraction with TRIzol reagent (15596026, Thermo Fisher, Waltham, MA, USA), and 1 μg total RNA was used for reverse transcription through SuperScript IV Reverse Transcriptase (18090200, Thermo Fisher, Waltham, MA, USA). ..

    Purification:

    Article Title: SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis
    Article Snippet: .. 120 ng of RNA from T. b. gambiense ELIANE strain for TgSGP or 40 ng of RNA from T. b. rhodesiense EATRO strain for SRA RNA production were retro-transcribed using pT19 oligonucleotide and SuperScript IV Reverse Transcriptase (Thermo Fisher; 200 U/ul 18090200) following standard protocols. cDNAs were purified with Ampure XP (A63880) following manufacturer instructions and eluted in 30 μL of nuclease free water. ..

    RNA Extraction:

    Article Title: Caspase-1 activates gasdermin A in non-mammals
    Article Snippet: The tissue samples were stored in RNAlater RNA Stabilization Solution (AM7020, Thermo Fisher, Waltham, MA, USA) at −80°C if the experiments were not processed immediately. .. Approximately 20 μg tissue samples were harvested for RNA extraction with TRIzol reagent (15596026, Thermo Fisher, Waltham, MA, USA), and 1 μg total RNA was used for reverse transcription through SuperScript IV Reverse Transcriptase (18090200, Thermo Fisher, Waltham, MA, USA). ..



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