superscript® iii reverse transcriptase synthesis kit (Thermo Fisher)

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Thermo Fisher superscript® iii reverse transcriptase synthesis kit
Superscript® Iii Reverse Transcriptase Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+transcriptase/superscript++iii+reverse+transcriptase+synthesis+kit/pmc12221670-72-18-24
Average 90 stars, based on 1 article reviews

superscript® iii reverse transcriptase synthesis kit - by Bioz Stars, 2026-09

90/100 stars

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Purification:

Article Title: Novel loss-of-function variants of TRAPPC2 manifesting X-linked spondyloepiphyseal dysplasia tarda: report of two cases
Article Snippet: After harvesting and lysing cells, lysates were first used to isolate total mRNA with RNeasy Mini Kit (Qiagen) according to a given instruction. .. Then, the purified RNA was subjected to produce cDNA library with reverse transcriptase reaction (SuperScript III First-Strand Synthesis Kit, Thermo). ..

cDNA Library Assay:

Reverse Transcription:

Article Title: Anti-galectin-9 antibody and methods of use thereof
Article Snippet: Anti-Galectin-9 P4D2 Monoclonal Antibody Fv Sequencing and Galectin-9 Modeling. .. RNA was extracted from the P4D2 Hybridomas using a chemical reagent for isolating biological material from organic tissue sold under the trademark TRIZOL® (ThermoFisher Scientific) and converted to cDNA with using a reverse transcriptase kit sold under the trademark SUPERSCRIPT® III First-Strand Synthesis System (Invitrogen). .. Contaminating VL cDNA from the P3X63Ag8.653 cells were labeled using 5′-biotinylated P3 CDR-L3 primers (5′-CAGCACATTAGGGAGCTTACACG-3′; SEQ ID NO:11) (IDT) and then removed using Streptavidin-linked beads.

Article Title: The Armadillo BTB Protein ABAP1 Is a Crucial Player in DNA Replication and Transcription of Nematode-Induced Galls
Article Snippet: The RNA was then treated with RNase-free DNase I (Thermo Fisher Scientific) before reverse transcription. .. One microgram of treated RNA was used to synthetize the cDNA using a SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) synthesis kit using oligo DT primers. .. This cDNA (1/20) was then amplified using SYBR Green I PCR Master No Rox (Eurogentec, Angers, France), and amplification of PCNA1, CDT1a, CDT1b, CYCB1;1, CDKA;1, SOG1, E2Fa , and TCP24 genes was performed with each specific primer pair ( ).

Article Title: Hyperlipidemia is necessary for the initiation and progression of atherosclerosis by severe periodontitis in mice
Article Snippet: .. Total RNA from mouse tissues was extracted using RNeasy micro kit (cat. no. 74106; Qiagen GmbH) and reverse-transcribed with the following cycle: 5 min at 65°C, 2 min at 25°C and 50 min at 45°C using SuperScript ® III Reverse Transcriptase Synthesis kit (cat. no. 18064014; Thermo Fisher Scientific, Inc.). .. Subsequently, qPCR was performed under the following conditions: Pre-incubation at 95°C for 2 min; amplification at 95°C for 15 sec, 57°C for 15 sec and 72°C for 1 min, for 55 cycles; melting curve at 95°C for 15 sec, 60°C for 1 min and 95°C for 15 sec using PowerUp TM SYBR Green Master Mix (cat. no. A25741; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

other:

Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling
Article Snippet: Total RNAs of tissues and cells were harvested using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse-transcribed using SuperScript® III Reverse Transcriptase Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Periodontitis-induced systemic inflammation exacerbates atherosclerosis partly via endothelial-mesenchymal transition in mice.
Article Snippet: Quantitative real-time polymerase chain reaction Total RNA from EndMT-undergoing HUVECs was extracted using Trizol-based methods (Thermo Fisher Scientific) and reversetranscribed using SuperScript® III Reverse Transcriptase Synthesis Kit (Thermo Fisher Scientific).

Article Title: Periodontitis-induced systemic inflammation exacerbates atherosclerosis partly via endothelial–mesenchymal transition in mice
Article Snippet: Total RNA from EndMT-undergoing HUVECs was extracted using Trizol-based methods (Thermo Fisher Scientific) and reverse-transcribed using SuperScript® III Reverse Transcriptase Synthesis Kit (Thermo Fisher Scientific).

Article Title: Rosuvastatin Prevents the Exacerbation of Atherosclerosis in Ligature-Induced Periodontal Disease Mouse Model
Article Snippet: Total RNA from mouse tissues or human/mouse cells was extracted using RNeasy micro kit (Qiagen, Hilden, Germany) and reverse-transcribed using SuperScript® III Reverse Transcriptase Synthesis Kit (Thermo Fisher Scientific).

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