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Valiant Co Ltd stet buffer
Stet Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 879 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stet+buffer/EDTA/pm42019502-152-38-57
Average 96 stars, based on 879 article reviews
stet buffer - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Sample Prep:

Article Title: Feasibility of Patient-Derived 3D Gastrointestinal Stromal Tumour Models as Alternatives for In Vivo Mouse Models
Article Snippet: .. Tissue lysates of PCTS were prepared in 2 mM EDTA in 70% EtOH ( v / v ) using lysing matrix D tubes with the FastPrep sample preparation system (MP Biomedicals, Santa Ana, CA, USA). .. The resulting supernatant was used to assess the viability of PCTSs using an ATP Bioluminescence-Assay (11699695001, Merck Chemicals GmbH, Darmstadt, Germany) according to the manufacturer’s instructions.

Lysis:

Article Title: Evaluation of safety and efficacy of an auxotrophic aroA mutant live attenuated vaccine against piscine Streptococcus agalactiae infection
Article Snippet: .. Briefly, log-phase S.agalactiae was collected, washed, and resuspended in lysis buffer (30 mM sodium acetate, 0.5% SDS, 1 mM EDTA, pH 5.5) and mechanically disrupted using Lysing Matrix B (MP Biomedicals). ..

Article Title: Identity Domains Reveal How Life Experiences and the Microbiome Shape Individuality and Uncover the Presence of Social Memory in Drosophila
Article Snippet: DNA extraction was performed using the DNeasy Blood & Tissue Kit (Qiagen, Cat. No. 69504), following the manufacturer’s protocol. .. Flies were homogenized in 200 μL of lysis buffer (20 mM Tris [pH 8.0], 2 mM EDTA, 1.2% Triton X-100) supplemented with lysozyme (20 mg/mL; MP Biomedicals, Cat. No. 210083401), followed by incubation for 90 minutes at 37°C. ..

Article Title: Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation.
Article Snippet: .. Cells were harvested at OD600[0.3–0.5] and pelleted at 3,220 xg for 12 min. Pellets were resuspended in 10 μL dPBS for HMW gDNA extraction using the Nanobind CBB kit (PacBio 102-301-900), including an extra lysis in 50 μL STET buffer [50 mM Tris-HCl, 50 mM EDTA, 8% (w/v) sucrose, 5% (w/v) Triton X-100, 10 mg.mL− 1 lysozyme (MP Biomedicals 100831), and 0.2 mg.mL− 1 lysostaphin (Sigma L7386)] as recommended by the manufacturer. ..

Article Title: Evaluation of safety and efficacy of an auxotrophic aroA mutant live attenuated vaccine against piscine Streptococcus agalactiae infection.
Article Snippet: .. Briefly, log-phase S.agalactiae was collected, washed and resuspended in lysis buffer (30 mM sodium acetate, 0.5% SDS, 1 mM EDTA, pH 5.5) and mechanically disrupted using Lysing Matrix B (MP Biomedicals). ..

Incubation:

Article Title: Identity Domains Reveal How Life Experiences and the Microbiome Shape Individuality and Uncover the Presence of Social Memory in Drosophila
Article Snippet: DNA extraction was performed using the DNeasy Blood & Tissue Kit (Qiagen, Cat. No. 69504), following the manufacturer’s protocol. .. Flies were homogenized in 200 μL of lysis buffer (20 mM Tris [pH 8.0], 2 mM EDTA, 1.2% Triton X-100) supplemented with lysozyme (20 mg/mL; MP Biomedicals, Cat. No. 210083401), followed by incubation for 90 minutes at 37°C. ..

Extraction:

Article Title: Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation.
Article Snippet: .. Cells were harvested at OD600[0.3–0.5] and pelleted at 3,220 xg for 12 min. Pellets were resuspended in 10 μL dPBS for HMW gDNA extraction using the Nanobind CBB kit (PacBio 102-301-900), including an extra lysis in 50 μL STET buffer [50 mM Tris-HCl, 50 mM EDTA, 8% (w/v) sucrose, 5% (w/v) Triton X-100, 10 mg.mL− 1 lysozyme (MP Biomedicals 100831), and 0.2 mg.mL− 1 lysostaphin (Sigma L7386)] as recommended by the manufacturer. ..

Isolation:

Article Title: Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease.
Article Snippet: .. PBMC isolation and flow cytometry Peripheral blood mononuclear cells (PBMC) were isolated from EDTA whole blood by density gradient centrifugation, using LSM (lymphocyte separation medium, MP Biomedicals, 0850494) according to the manufacturer’s recommendations, frozen in Cryostor CS10 (Stemcell technologies, 07959) and stored in liquid nitrogen. .. Frozen PBMCs were thawed in complete RPMI, washed twice, and stained with live/dead marker (fixable viability dye eFluor780, eBioscience 65-0865-14) in the presence of an Fc receptor binding inhibitor cocktail (eBioscience, 14-9161-73).

Flow Cytometry:

Article Title: Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease.
Article Snippet: .. PBMC isolation and flow cytometry Peripheral blood mononuclear cells (PBMC) were isolated from EDTA whole blood by density gradient centrifugation, using LSM (lymphocyte separation medium, MP Biomedicals, 0850494) according to the manufacturer’s recommendations, frozen in Cryostor CS10 (Stemcell technologies, 07959) and stored in liquid nitrogen. .. Frozen PBMCs were thawed in complete RPMI, washed twice, and stained with live/dead marker (fixable viability dye eFluor780, eBioscience 65-0865-14) in the presence of an Fc receptor binding inhibitor cocktail (eBioscience, 14-9161-73).

Gradient Centrifugation:

Article Title: Adult patients with autoinflammation of unknown origin partially phenocopy the immune presentation of Still's disease.
Article Snippet: .. PBMC isolation and flow cytometry Peripheral blood mononuclear cells (PBMC) were isolated from EDTA whole blood by density gradient centrifugation, using LSM (lymphocyte separation medium, MP Biomedicals, 0850494) according to the manufacturer’s recommendations, frozen in Cryostor CS10 (Stemcell technologies, 07959) and stored in liquid nitrogen. .. Frozen PBMCs were thawed in complete RPMI, washed twice, and stained with live/dead marker (fixable viability dye eFluor780, eBioscience 65-0865-14) in the presence of an Fc receptor binding inhibitor cocktail (eBioscience, 14-9161-73).



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