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stet lysis buffer  (Roche)


Bioz Manufacturer Symbol Roche manufactures this product  
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    Structured Review

    Roche stet lysis buffer
    Stet Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stet+buffer/pm24723692-41-4-30
    Average 86 stars, based on 1 article reviews
    stet lysis buffer - by Bioz Stars, 2026-11
    86/100 stars

    Images

    Related Articles

    Gentle:

    Article Title: GGNBP2 regulates histone ubiquitination and methylation in spermatogenesis.
    Article Snippet: .. The nuclei were washed by gentle resuspension using wide orifice tips in extraction buffer (20 mMTrisCl [pH 7.5], 0.25% Triton X-100, 1 Complete-EDTA free protease inhibitor mixture [Roche, Mannheim, Germany]) containing increasing concentrations of NaCl. ..

    Extraction:

    Article Title: GGNBP2 regulates histone ubiquitination and methylation in spermatogenesis.
    Article Snippet: .. The nuclei were washed by gentle resuspension using wide orifice tips in extraction buffer (20 mMTrisCl [pH 7.5], 0.25% Triton X-100, 1 Complete-EDTA free protease inhibitor mixture [Roche, Mannheim, Germany]) containing increasing concentrations of NaCl. ..

    Protease Inhibitor:

    Article Title: GGNBP2 regulates histone ubiquitination and methylation in spermatogenesis.
    Article Snippet: .. The nuclei were washed by gentle resuspension using wide orifice tips in extraction buffer (20 mMTrisCl [pH 7.5], 0.25% Triton X-100, 1 Complete-EDTA free protease inhibitor mixture [Roche, Mannheim, Germany]) containing increasing concentrations of NaCl. ..

    Article Title: PIEZO1 targeting in macrophages boosts phagocytic activity and foam cell apoptosis in atherosclerosis.
    Article Snippet: .. Monocyte-derived macrophages were lysed in a buffer containing 50 mmol/L HEPES (pH 7.4), 1% Triton X‐100 (v/v), complete protease inhibitor cocktail, and PhosSTOP phosphatase inhibitor cocktail (both from Roche Diagnostics). .. Equal amounts of protein were loaded to SDS‐PAGE using 4–20% Criterion XT Bis‐Tris precast gels (Bio‐Rad, Hercules, CA) followed by transfer onto polyvinylidene fluoride membrane (Immobilon‐P; Millipore).

    Article Title: Bacterial Production of CDKL5 Catalytic Domain: Insights in Aggregation, Internal Translation and Phosphorylation Patterns.
    Article Snippet: .. The cellular paste was resuspended in 13 mL binding buffer (25 mM Hepes pH 7.7, 15 mM imidazole, 0.7 M NaCl, 1% Triton X-100) supplemented with 10 mg/mL lysozyme, and complete EDTA-free protease inhibitor cocktail (Roche, Basel, Switzerland). ..

    Article Title: Ubiquitination and degradation of plant helper NLR by the Ralstonia solanacearum effector RipV2 overcome tomato bacterial wilt resistance.
    Article Snippet: .. The tissues were lysed with Co-IP buffer containing 20 mM TrisHCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 10% glycerol, 0.5% Triton X-100, and protease inhibitor cocktail (Roche, Cat. 04693116001). .. After centrifuging at 12,000 rpm for 15 min, supernatant was incubated with Anti-Flag Affinity Gel (Bimake, Cat. B23102) for 3 h at 4 C. Then the affinity gel was collected and washed three times with washing buffer containing 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, and 0.1% Triton X-100, and protease inhibitor.

    Article Title: p53-armed oncolytic virotherapy induces abscopal effect in osteosarcoma by promoting immunogenic cell death
    Article Snippet: .. Cells were then infected with OBP-301 or OBP-702 at an MOI of 0, 1, 5, 10, 50, or 100 PFUs/cell for 72 h. Whole-cell lysates were prepared in lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Triton X-100) containing a protease inhibitor cocktail (Complete Mini; Roche, Indianapolis, IN, USA). .. Proteins were electrophoresed on 6%–15% sodium dodecyl sulfate-polyacrylamide gels and then transferred onto polyvinylidene difluoride membranes (Hybond-P; GE Healthcare, Buckinghamshire, UK).

    Article Title: Modulation of the K/BxN arthritis mouse model and the effector functions of human fibroblast-like synoviocytes by liver X receptors.
    Article Snippet: Recombinant human TNF, IL-1β, and CXCL12 were obtained from Immunotools. .. Western blot For Western blot analyses, resting RA FLS (1 × 106 cells) were lysed using TENT: Tris HCl (50 mM), EDTA (5 mM), NaCl (150 mM), and 1% Triton X-100, supplemented with a complete protease inhibitor mixture (Roche Diagnostics) for 15 min on ice. ..

    Article Title: PIEZO1 targeting in macrophages boosts phagocytic activity and foam cell apoptosis in atherosclerosis
    Article Snippet: .. Monocyte-derived macrophages were lysed in a buffer containing 50 mmol/L HEPES (pH 7.4), 1% Triton X‐100 (v/v), complete protease inhibitor cocktail, and PhosSTOP phosphatase inhibitor cocktail (both from Roche Diagnostics). .. Equal amounts of protein were loaded to SDS‐PAGE using 4–20% Criterion XT Bis‐Tris precast gels (Bio‐Rad, Hercules, CA) followed by transfer onto polyvinylidene fluoride membrane (Immobilon‐P; Millipore).

    Binding Assay:

    Article Title: Bacterial Production of CDKL5 Catalytic Domain: Insights in Aggregation, Internal Translation and Phosphorylation Patterns.
    Article Snippet: .. The cellular paste was resuspended in 13 mL binding buffer (25 mM Hepes pH 7.7, 15 mM imidazole, 0.7 M NaCl, 1% Triton X-100) supplemented with 10 mg/mL lysozyme, and complete EDTA-free protease inhibitor cocktail (Roche, Basel, Switzerland). ..

    Co-Immunoprecipitation Assay:

    Article Title: Ubiquitination and degradation of plant helper NLR by the Ralstonia solanacearum effector RipV2 overcome tomato bacterial wilt resistance.
    Article Snippet: .. The tissues were lysed with Co-IP buffer containing 20 mM TrisHCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 10% glycerol, 0.5% Triton X-100, and protease inhibitor cocktail (Roche, Cat. 04693116001). .. After centrifuging at 12,000 rpm for 15 min, supernatant was incubated with Anti-Flag Affinity Gel (Bimake, Cat. B23102) for 3 h at 4 C. Then the affinity gel was collected and washed three times with washing buffer containing 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, and 0.1% Triton X-100, and protease inhibitor.

    Infection:

    Article Title: p53-armed oncolytic virotherapy induces abscopal effect in osteosarcoma by promoting immunogenic cell death
    Article Snippet: .. Cells were then infected with OBP-301 or OBP-702 at an MOI of 0, 1, 5, 10, 50, or 100 PFUs/cell for 72 h. Whole-cell lysates were prepared in lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Triton X-100) containing a protease inhibitor cocktail (Complete Mini; Roche, Indianapolis, IN, USA). .. Proteins were electrophoresed on 6%–15% sodium dodecyl sulfate-polyacrylamide gels and then transferred onto polyvinylidene difluoride membranes (Hybond-P; GE Healthcare, Buckinghamshire, UK).

    Lysis:

    Article Title: p53-armed oncolytic virotherapy induces abscopal effect in osteosarcoma by promoting immunogenic cell death
    Article Snippet: .. Cells were then infected with OBP-301 or OBP-702 at an MOI of 0, 1, 5, 10, 50, or 100 PFUs/cell for 72 h. Whole-cell lysates were prepared in lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Triton X-100) containing a protease inhibitor cocktail (Complete Mini; Roche, Indianapolis, IN, USA). .. Proteins were electrophoresed on 6%–15% sodium dodecyl sulfate-polyacrylamide gels and then transferred onto polyvinylidene difluoride membranes (Hybond-P; GE Healthcare, Buckinghamshire, UK).

    Western Blot:

    Article Title: Modulation of the K/BxN arthritis mouse model and the effector functions of human fibroblast-like synoviocytes by liver X receptors.
    Article Snippet: Recombinant human TNF, IL-1β, and CXCL12 were obtained from Immunotools. .. Western blot For Western blot analyses, resting RA FLS (1 × 106 cells) were lysed using TENT: Tris HCl (50 mM), EDTA (5 mM), NaCl (150 mM), and 1% Triton X-100, supplemented with a complete protease inhibitor mixture (Roche Diagnostics) for 15 min on ice. ..



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