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one step tb green primescript rt pcr kit ii  (TaKaRa)


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    Structured Review

    TaKaRa one step tb green primescript rt pcr kit ii
    One Step Tb Green Primescript Rt Pcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/step+primescript+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pmc13657405-173-8-19
    Average 98 stars, based on 10975 article reviews
    one step tb green primescript rt pcr kit ii - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: The copper transporter protein SLC31A1-mediated copper metabolism imbalance drives cuproptosis through the SIRT1/HMGB1 pathway in heart failure.
    Article Snippet: Objective: Disruption of copper (Cu) homeostasis can induce pathological alterations, including heart failure (HF).. Solute carrier family 31 member 1 (SLC31A1) is a significant Cu transporter protein affecting Cu absorption at cell membranes.. This study investigated the role of SLC31A1-regulated Cu metabolism in HF and the underlying

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus.
    Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus).

    Article Title: Establishment and preliminary evaluation of an RT-ERA combined with CRISPR/Cas12a assay for detection of H5 and H7 influenza viruses
    Article Snippet: .. RT-qPCR was performed using the 2×One Step PrimeScript RT-PCR Kit (Takara) with the following conditions: reverse transcription at 42 °C for 5 min, initial denaturation at 95 °C for 10 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Fluorescence signals were acquired at the 60 °C annealing-extension step. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The copper transporter protein SLC31A1-mediated copper metabolism imbalance drives cuproptosis through the SIRT1/HMGB1 pathway in heart failure.
    Article Snippet: Objective: Disruption of copper (Cu) homeostasis can induce pathological alterations, including heart failure (HF).. Solute carrier family 31 member 1 (SLC31A1) is a significant Cu transporter protein affecting Cu absorption at cell membranes.. This study investigated the role of SLC31A1-regulated Cu metabolism in HF and the underlying

    Article Title: The capsid proteins of orthoflavivirus are interchangeable for West Nile virus assembly.
    Article Snippet: .. Total RNAs of WNV-DV2mC-P10-A/B/C-infected cells were extracted, and RT-PCR was then carried out using the PrimeScript One Step RT-PCR Kit (Takara) with primers to amplify the fragments spanning from 5′ UTR to 3′ UTR. ..

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus.
    Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus).

    Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy
    Article Snippet: .. Measurements of host gene expression were performed using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Takara). ..

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Article Title: Establishment and preliminary evaluation of an RT-ERA combined with CRISPR/Cas12a assay for detection of H5 and H7 influenza viruses
    Article Snippet: .. RT-qPCR was performed using the 2×One Step PrimeScript RT-PCR Kit (Takara) with the following conditions: reverse transcription at 42 °C for 5 min, initial denaturation at 95 °C for 10 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Fluorescence signals were acquired at the 60 °C annealing-extension step. ..

    cDNA Synthesis:

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    SYBR Green Assay:

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    Polymerase Chain Reaction:

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    One Step RT-PCR:

    Article Title: The capsid proteins of orthoflavivirus are interchangeable for West Nile virus assembly.
    Article Snippet: .. Total RNAs of WNV-DV2mC-P10-A/B/C-infected cells were extracted, and RT-PCR was then carried out using the PrimeScript One Step RT-PCR Kit (Takara) with primers to amplify the fragments spanning from 5′ UTR to 3′ UTR. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Gene Expression:

    Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy
    Article Snippet: .. Measurements of host gene expression were performed using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Takara). ..

    Concentration Assay:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Spectrophotometry:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Synthesized:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Quantitative RT-PCR:

    Article Title: Establishment and preliminary evaluation of an RT-ERA combined with CRISPR/Cas12a assay for detection of H5 and H7 influenza viruses
    Article Snippet: .. RT-qPCR was performed using the 2×One Step PrimeScript RT-PCR Kit (Takara) with the following conditions: reverse transcription at 42 °C for 5 min, initial denaturation at 95 °C for 10 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Fluorescence signals were acquired at the 60 °C annealing-extension step. ..

    Fluorescence:

    Article Title: Establishment and preliminary evaluation of an RT-ERA combined with CRISPR/Cas12a assay for detection of H5 and H7 influenza viruses
    Article Snippet: .. RT-qPCR was performed using the 2×One Step PrimeScript RT-PCR Kit (Takara) with the following conditions: reverse transcription at 42 °C for 5 min, initial denaturation at 95 °C for 10 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Fluorescence signals were acquired at the 60 °C annealing-extension step. ..



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