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2 3 5 triphenyltetrazolium chloride staining solution  (Servicebio Inc)

 
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    Servicebio Inc 2 3 5 triphenyltetrazolium chloride staining solution
    2 3 5 Triphenyltetrazolium Chloride Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/2+3+5+chloride+triphenyltetrazolium+ttc/pmc13255054-17-0-5
    Average 86 stars, based on 1 article reviews
    2 3 5 triphenyltetrazolium chloride staining solution - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. 2,3,5-Triphenyltetrazolium chloride staining solution , Servicebio , Cat# G1017. .. 4′,6-diamidino-2-phenylindole , Sigma-Aldrich , Cat# 28718-90-3.

    Article Title: Renal denervation attenuates cardiac inflammatory responses via the afferent renal-splenic nerve axis after myocardial ischemic injury.
    Article Snippet: .. The harvested hearts were sliced and subsequently stained with pre-warmed 2,3,5-triphenyltetrazolium chloride (TTC) dye (ServiceBio, China) for 30 min at a temperature of 37 °C. .. Thereafter, the slices were dried, and images were captured using a Leica microscope.

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    Article Title: Renal denervation attenuates cardiac inflammatory responses via the afferent renal-splenic nerve axis after myocardial ischemic injury
    Article Snippet: .. The harvested hearts were sliced and subsequently stained with pre-warmed 2,3,5-triphenyltetrazolium chloride (TTC) dye (ServiceBio, China) for 30 min at a temperature of 37 °C. .. Thereafter, the slices were dried, and images were captured using a Leica microscope.

    Article Title: Brain-derived HMGB1 mediates periodontal inflammation after ischemic stroke through the STAT3/NLRP3 inflammasome pathway.
    Article Snippet: Ischemic stroke is known to cause immunosuppression and may exacerbate peripheral conditions such as periodontitis, yet its direct impact on periodontal tissues remains unclear.. Using a transient middle cerebral artery occlusion (MCAO) mouse model, we showed that stroke rapidly induced periodontal inflammation, including epithelial disruption, alveolar bone loss, increased osteoclasts, and activation of the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome.. Meanwhile, we found that neuronal high-mobility group box 1 (HMGB1) translocated from the nucleus and was released after stroke, coinciding with disruption of the blood–brain barrier and elevated circulating HMGB1.

    Recombinant:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    CCK-8 Assay:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    Cytotoxicity Assay:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    TUNEL Assay:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    BIA-KA:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    Enzyme-linked Immunosorbent Assay:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    RNA Sequencing:

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Cherp Proteintech Cat# 25389-1-AP; RRID: AB_2880055 Mouse anti-Bcl2 Proteintech Cat# 68103-1-Ig; RRID: AB_2923635 Rabbit anti-Bax Proteintech Cat# 50599-2-Ig; RRID: AB_2061561 Mouse anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID: AB_2107436 HRP-conjugated host anti- mouse IgG secondary antibodies Proteintech Cat# SA00001-0 Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody Beyotime Cat# A0423 Biological samples Human peripheral blood Affiliated Hospital of North Sichuan Medical College N/A Mouse brain tissue This paper N/A Primary neurons This paper N/A Chemicals, peptides, and recombinant proteins 2,3,5-Triphenyltetrazolium chloride staining solution Servicebio Cat# G1017 4′,6-diamidino-2-phenylindole Sigma-Aldrich Cat# 28718-90-3 DMEM Gibco Cat# 11965092 B-27 Gibco Cat# A3582801 L-glutamine Invitrogen Cat# A2916801 Cell Counting Kit-8 Beyotime Cat# C0037 RIPA buffer Beyotime Cat# P0013B Fluo-4 AM Beyotime Cat# S1060 Critical commercial assays CytoTox 96® Non-Radioactive Cytotoxicity Assay Beyotime Cat# G1780 TUNEL assay Thermo Fisher Scientific Cat# C10618 BCA assay Beyotime Cat# P0009 Human Il18 ELISA kit Cusabio Cat# CSB-E07450h Deposited data scRNA-seq GEO database GSE174574 Bluk RNA-seq GEO database GSE58294 Bluk RNA-seq GEO database GSE61616 Bluk RNA-seq GEO database GSE16561 Experimental models: Cell lines Primary neurons This paper N/A Experimental models: Organisms/strains C57BL/6 mice Gem Pharmatech Strain#. .. N000013 Recombinant DNA pLKO.1-shCherp Sangon Biotech N/A pLKO.1-NC Sangon Biotech N/A Software and algorithms ImageJ NIH https://imagej.net/software/fiji/downloads GraphPad Prizm GraphPad Software https://www.graphpad.com/ R (version 4.4.1) The R Project for Statistical Computing https://www.r-project.org/ Seurat (version 4.3.0.1) R package N/A (Continued on next page) iScience 29, 116257, June 19, 2026 e1

    other:

    Article Title: Targeted exosome-delivered CD151 siRNA maintains brain endothelial cell immune homeostasis to alleviate blood-brain barrier disruption after ischemic stroke.
    Article Snippet: Infarct volume was measured using 2% 2,3,5-triphenyltetrazolium chloride (TTC) solution (Servicebio, Wuhan, China), as previously described [32].



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    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Inhibition, CCK-8 Assay, Real-time Monitoring, Fluorescence, Flow Cytometry, Analysis, Staining

    Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Analysis, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Co-Immunoprecipitation Assay, Significance Assay