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c1005 staining solution  (Beyotime)


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    Structured Review

    Beyotime c1005 staining solution
    C1005 Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/DAPI+Staining+Solution/pm42880593-220-13-19
    Average 99 stars, based on 2090 article reviews
    c1005 staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Staining:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Hierarchically sequential targeting nanoplatform suppresses NETosis-promoted angiogenesis for corneal neovascularization therapy.
    Article Snippet: The Phorbol 12-myristate 13-acetate (PMA, S1819) was purchased from Beyotime (Shanghai, China). .. The 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride Jo ur na l P re -p ro of (DAPI, C1005) Staining Solution was purchased from Beyotime (Shanghai, China). .. The clamidine hydrochloride was purchased from Sparkjade (SJ-MX0367A, Shandong, China).

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding
    Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. DAPI Staining Solution , Beyotime , C1006. .. mTeSR1 medium , STEMCELL , 85850.

    Incubation:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Glass:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Inverted Microscopy:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Fluorescence:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    other:

    Article Title: Lacticaseibacillus rhamnosus GG enhances gefitinib efficacy and reduces treatment-related toxicity via propionyl-L-carnitine regulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Propionyl-L-carnitine (PLC) Aladdin Biochemical Technology, Shanghai, China CAS: 20064-19-1 Acetonitrile Xilong Scientific Co., Ltd., China CAS: 75-05-8 Formic acid Xilong Scientific Co., Ltd., China CAS: 64-18-6 Methotrexate Yuanye Bio-Technology, Shanghai, China CAS: 59-05-2 Luciferin sodium salt Yuanye Bio-Technology, Shanghai, China Cat#: B25437 Sodium carboxymethylcellulose (CMC-Na) Solarbio Life Sciences, Beijing, China CAS: 9004-32-4 Universal Tissue Fixative (Neutral) Servicebio, Wuhan, China Cat#: G1101 Trypsin Gibco Cat#: 25200-056 Fetal bovine serum Gibco Cat#: A5669701 Penicillin-streptomycin Gibco Cat#: 15140122 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#: 11965092 RPMI 1640 medium Gibco Cat#: C11875500BT BSA blocking solution Thermo Fisher Scientific Cat#: 37525 RIPA lysis buffer Thermo Fisher Scientific Cat#: 89901 RIPA buffer Beyotime Biotechnology Cat#: P0013B PMSF Beyotime Biotechnology Cat#: ST507 Protease/phosphatase inhibitor cocktail Beyotime Biotechnology Cat#: P1045 0.1% Triton X-100 Beyotime Biotechnology Cat#: P0096 DAPI staining solution Beyotime Biotechnology Cat#: C1006 Anhydrous Sodium Sulfate Xilong Scientific Co., Ltd., China CAS: 7757-82-6 Sodium Chloride (NaCl) Xilong Scientific Co., Ltd., China CAS: 7647-14-5 Dilute Sulfuric Acid (H2SO4) Xilong Scientific Co., Ltd., China CAS: 7664-93-9 n-Hexane Xilong Scientific Co., Ltd., China CAS: 110-54-3 Collagenase Type IV Sigma-Aldrich Cat#: C4-28 DNase I Beyotime Biotechnology Cat#: D7073 Critical commercial assays CCK-8 Kit MedChemExpress Cat#: HY-K0301 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat#: C0009S Annexin V-FITC/PI Apoptosis Detection Kit Huiyucheng Biotechnology, Wuhan, China Cat#: HYC2019 BCA Protein Assay Kit Thermo Fisher Scientific Cat#: 23225 TUNEL Assay Kit Beyotime Biotechnology Cat#: C1098 DAB Substrate Kit Beyotime Biotechnology Cat# P0202 Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat#: 32106 CEA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9M0916 CYFRA21-1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY926701a GLUT1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM11861 LDHA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM1898 Mitochondrial Complex I Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737601a Mitochondrial Complex II Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737701a Mitochondrial Complex III Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737801a Mitochondrial Complex IV Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737901a Mitochondrial Complex V Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737011a Propionyl-L-carnitine (PLC) ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY972791a Mouse ROS ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XYM9437001 Mouse IKKα ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9438821a (Continued on next page) 18 Cell Reports 45, 118068, October 27, 2026



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    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of <t>calcein</t> and Annexin V fluorescence results <t>showing</t> <t>mPTP</t> opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).
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    Image Search Results


    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Inhibition, CCK-8 Assay, Real-time Monitoring, Fluorescence, Flow Cytometry, Analysis, Staining

    Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Analysis, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Co-Immunoprecipitation Assay, Significance Assay