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fix solution  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec fix solution
    Fix Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/Inside+Stain+Kit/pm42415147-126-6-14
    Average 97 stars, based on 351 article reviews
    fix solution - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Aquaporin-4–Specific T Cell Responses in NMOSD Revealed by mRNA-Engineered Dendritic Cells
    Article Snippet: .. After fixation and permeabilization using Inside Fix (Miltenyi Biotec, 130-090-477) for 20 min, intracellular staining was performed using antibodies listed in Supplementary Table 2 for 20 minutes. ..

    Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Germany) and SSEA4 (1:50, #130-124-073, Miltenyi Biotec, Bergisch Gladbach, Germany) in 25 μl PEB buffer (PBS + 0.5% BSA) for 10 min at 4°C, washed in 500 μl PEB buffer and centrifuged at 200xg for 5 min. .. Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After washing in 500 μl PEB buffer and centrifugation at 200xg cells were stained with the nuclear stem cell markers OCT3/4 (1:50, #130-117-821, Miltenyi Biotec, Bergisch Gladbach, Germany) and SOX2 (1:50, #130-120-790, Miltenyi Biotec, Bergisch Gladbach, Germany) in inside perm solution for 10 min at RT. ..

    Article Title: Mavacamten improves energy balance in a pre-clinical model of RASopathy-associated hypertrophic cardiomyopathy.
    Article Snippet: Pediatric Research Troponin detection, relative cell size, and mitochondrial content by flow cytometry Single-cell suspensions of hiPSC-derived cardiomyocytes were obtained using 0.05% trypsin-EDTA (Thermo Fisher Scientific) and stained with Zombie NIRTM viability dye (BioLegend, San Diego, California) following the manufacturer’s instructions. .. Cells were fixed and permeabilized with the Inside Stain Kit (Miltenyi Biotec, Germany) for 10min at room temperature, followed by a 10-min incubation with cTnT-APC or control IgG1-APC REAfinity antibody (Miltenyi Biotech). .. Data were acquired with a BD LSR Fortessa X-20 (BD Biosciences, France) flow cytometer.

    Article Title: Vaccine-expanded plasmablast-like B cells are associated with response to dendritic cell therapy in metastatic melanoma
    Article Snippet: .. The Inside Stain Kit (Miltenyi Biotec) was used for intracellular staining. ..

    Article Title: HLA-E-Directed Accumulation of KIR + NKG2C + NK Cells upon HCMV Peptide Presentation In Vitro: Association with the Ex Vivo Phenotype.
    Article Snippet: .. The level of intracellular GrB was assessed using a kit for cellular fixation and intracellular staining (Inside Stain Kit, Miltenyi Biotech) with granzyme B AF647 Abs (clone GB11, Biolegend, San Jose, CA, USA). .. To assess the proportion of non-viable cells in spheroids, SytoxBlue viable dye (Invitrogen, Carlsbad, CA, USA) was added to samples immediately before the flow acquisition.

    Article Title: Early adjunct anti-PD-L1 immunotherapy improves outcomes and restores infection-induced immune paralysis in mice with invasive pulmonary mucormycosis.
    Article Snippet: .. For the T cell panel, the Inside Stain Kit (Miltenyi Biotec) was used according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Germany) and SSEA4 (1:50, #130-124-073, Miltenyi Biotec, Bergisch Gladbach, Germany) in 25 μl PEB buffer (PBS + 0.5% BSA) for 10 min at 4°C, washed in 500 μl PEB buffer and centrifuged at 200xg for 5 min. .. Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After washing in 500 μl PEB buffer and centrifugation at 200xg cells were stained with the nuclear stem cell markers OCT3/4 (1:50, #130-117-821, Miltenyi Biotec, Bergisch Gladbach, Germany) and SOX2 (1:50, #130-120-790, Miltenyi Biotec, Bergisch Gladbach, Germany) in inside perm solution for 10 min at RT. ..

    Article Title: Mavacamten improves energy balance in a pre-clinical model of RASopathy-associated hypertrophic cardiomyopathy.
    Article Snippet: Pediatric Research Troponin detection, relative cell size, and mitochondrial content by flow cytometry Single-cell suspensions of hiPSC-derived cardiomyocytes were obtained using 0.05% trypsin-EDTA (Thermo Fisher Scientific) and stained with Zombie NIRTM viability dye (BioLegend, San Diego, California) following the manufacturer’s instructions. .. Cells were fixed and permeabilized with the Inside Stain Kit (Miltenyi Biotec, Germany) for 10min at room temperature, followed by a 10-min incubation with cTnT-APC or control IgG1-APC REAfinity antibody (Miltenyi Biotech). .. Data were acquired with a BD LSR Fortessa X-20 (BD Biosciences, France) flow cytometer.

    Centrifugation:

    Article Title: Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firing.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Germany) and SSEA4 (1:50, #130-124-073, Miltenyi Biotec, Bergisch Gladbach, Germany) in 25 μl PEB buffer (PBS + 0.5% BSA) for 10 min at 4°C, washed in 500 μl PEB buffer and centrifuged at 200xg for 5 min. .. Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After washing in 500 μl PEB buffer and centrifugation at 200xg cells were stained with the nuclear stem cell markers OCT3/4 (1:50, #130-117-821, Miltenyi Biotec, Bergisch Gladbach, Germany) and SOX2 (1:50, #130-120-790, Miltenyi Biotec, Bergisch Gladbach, Germany) in inside perm solution for 10 min at RT. ..

    Control:

    Article Title: Mavacamten improves energy balance in a pre-clinical model of RASopathy-associated hypertrophic cardiomyopathy.
    Article Snippet: Pediatric Research Troponin detection, relative cell size, and mitochondrial content by flow cytometry Single-cell suspensions of hiPSC-derived cardiomyocytes were obtained using 0.05% trypsin-EDTA (Thermo Fisher Scientific) and stained with Zombie NIRTM viability dye (BioLegend, San Diego, California) following the manufacturer’s instructions. .. Cells were fixed and permeabilized with the Inside Stain Kit (Miltenyi Biotec, Germany) for 10min at room temperature, followed by a 10-min incubation with cTnT-APC or control IgG1-APC REAfinity antibody (Miltenyi Biotech). .. Data were acquired with a BD LSR Fortessa X-20 (BD Biosciences, France) flow cytometer.

    other:

    Article Title: Antigen-restricted Candida albicans Th17 cells link oral-gut immunity and adopt pathogenic features during intestinal inflammation.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were freshly isolated from EDTA blood on the day of blood donation by density gradient centrifugation (Biocoll; Biochrom, Berlin, Germany).



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    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of <t>calcein</t> and Annexin V fluorescence results <t>showing</t> <t>mPTP</t> opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).
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    Image Search Results


    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Inhibition, CCK-8 Assay, Real-time Monitoring, Fluorescence, Flow Cytometry, Analysis, Staining

    Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Analysis, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Co-Immunoprecipitation Assay, Significance Assay