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4 6 diamidino 2 phenylindole dapi staining solution  (Beyotime)


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    Structured Review

    Beyotime 4 6 diamidino 2 phenylindole dapi staining solution
    4 6 Diamidino 2 Phenylindole Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2079 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/DAPI+Staining+Solution/pm42844620-90-10-14
    Average 99 stars, based on 2079 article reviews
    4 6 diamidino 2 phenylindole dapi staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: TRPC6-TXNIP-NLRP3 signaling axis is involved in type 2 diabetes-associated cognitive dysfunction and the intervention of ginsenoside Rg1.
    Article Snippet: Type 2 diabetes-associated cognitive dysfunction (TDACD) poses a significant global public health challenge.. However, the core molecular mechanisms underlying its pathogenesis remain incompletely understood, and the development of effective therapeutic interventions continues to face considerable obstacles.. Here, we identify the role of the TRPC6-TXNIP-NLRP3 signaling axis in TDACD and to investigate the protective effects and mechanisms of ginsenoside Rg1 against TDACD.

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Article Title: Targeted delivery of lupeol via hyaluronic acid-modified ZIF-8 for anti-hepatic fibrosis therapy
    Article Snippet: Quantitative analysis was performed using flow cytometry (Beckman Coulter, USA). .. A DAPI Staining Solution (Beyotime Biotechnology, Shanghai, China) was used to perform the cell apoptosis shape assay on LX-2 cells according to the manufacturer's instructions. .. Cells were seeded into six-well plates at a density of 1.0 × 10 6 cells per well and cultured for 24 h. The next day, cells were treated with different media containing Lupeol, Lupeol@ZIF-8 and HA/Lupeol@ZIF-8 (60 μM lupeol), with blank and model groups included as controls, and incubated for an additional 24 h. For qualitative analysis, DAPI solution was added 24 h after treatment, and fluorescence signals were recorded using a fluorescence microscope (Olympus, Tokyo, Japan).

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
    Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding
    Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. DAPI Staining Solution , Beyotime , C1006. .. mTeSR1 medium , STEMCELL , 85850.

    Article Title: EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT
    Article Snippet: .. Finally, nuclei were stained with DAPI staining solution (Beyotime, C1005). .. The expression intensity of target proteins was observed under a CSU-X1 laser confocal microscope (Nikon, Japan).

    Inverted Microscopy:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Fluorescence:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Immunostaining:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Blocking Assay:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    BIA-KA:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Protein Concentration:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    Membrane:

    Article Title: Computational-experimental integration reveals PPARγ/Nrf2-GPX4 dual-targeting by combined selenomethionine-magnolol as a novel therapy for sarcopenic obesity.
    Article Snippet: Sarcopenic obesity (SO), characterized by concurrent muscle loss and excessive adiposity, lacks effective interventions.. This study investigated the enhanced effects of the selenomethionine (Se-Met) and magnolol (Mag) combination against SO using an integrated computational-experimental approach.. Network pharmacology and molecular docking predicted peroxisome proliferator-activated receptor γ (PPARγ) and the nuclear factor erythroid 2-related factor 2 glutathione peroxidase 4 (Nrf2 GPX4) axis as core targets, which was corroborated by single-cell transcriptomic data analysis showing their co-expression in muscle progenitor cells.

    TUNEL Assay:

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Incubation:

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Bicinchoninic Acid Protein Assay:

    Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
    Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).



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    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) <t>Representative</t> <t>JC-1</t> staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.
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    Beyotime giemsa staining
    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) <t>Representative</t> <t>JC-1</t> staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.
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    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) <t>Representative</t> <t>JC-1</t> staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.
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    Image Search Results


    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) Representative JC-1 staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

    Journal: Journal of Inflammation Research

    Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

    doi: 10.2147/JIR.S623377

    Figure Lengend Snippet: HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) Representative JC-1 staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

    Article Snippet: After 24 h, 300 μL JC-1 staining solution (C2006; Beyotime) was added, with additional 20-min cultivation in the dark at 37°C.

    Techniques: Fluorescence, Control, Staining, Polymer, Morphology, Derivative Assay

    Pharmacological suppression of protein palmitoylation attenuates HG- and PA-induced ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs by the use of DCFH-DA fluorescence probe in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C and D ) Measurement of MDA and GSH levels in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E ) Representative JC-1 staining photographs in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantifying MMP changes. ( G ) Detection of ATP in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( H ) TEM for investigating the changes in mitochondrial morphology in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 500 nm. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Journal of Inflammation Research

    Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

    doi: 10.2147/JIR.S623377

    Figure Lengend Snippet: Pharmacological suppression of protein palmitoylation attenuates HG- and PA-induced ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs by the use of DCFH-DA fluorescence probe in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C and D ) Measurement of MDA and GSH levels in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E ) Representative JC-1 staining photographs in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantifying MMP changes. ( G ) Detection of ATP in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( H ) TEM for investigating the changes in mitochondrial morphology in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 500 nm. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: After 24 h, 300 μL JC-1 staining solution (C2006; Beyotime) was added, with additional 20-min cultivation in the dark at 37°C.

    Techniques: Fluorescence, Control, Staining, Polymer, Morphology, Derivative Assay