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transcript variant 1  (OriGene)


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    Structured Review

    OriGene transcript variant 1
    Transcript Variant 1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src/SRC+(NM_005417)+Human+Untagged+Clone/pm42031710-117-24-27
    Average 94 stars, based on 3 article reviews
    transcript variant 1 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: All-trans-retinoic acid activates the pro-invasive Src-YAP-Interleukin 6 axis in triple-negative MDA-MB-231 breast cancer cells while cerivastatin reverses this action
    Article Snippet: Transfections were carried using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions. .. Src plasmid was from Origene (sc125208). .. Interference of Src was accomplished using a pre-designed ON-TARGET SMART pool from ThermoScientific (L-003175).

    Article Title: Targeting SRC enhances differentiation and promotes multifaceted cell death mechanisms in recurrent group 3 medulloblastoma.
    Article Snippet: To restore SRC expression in SRC knockout cells, cells were treated with 0.5 μM doxycycline for 24h to induce knock-out before receiving 250μL of plasmid DNA with lipofectamine 3000 and P3000 complex (Invitrogen, Cat No. L3000015) prepared in Opti-MEM media according to manufacturer’s instructions. .. Cells received 0.5μg/mL of SRC (SRC (untagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog AR TI CL E IN P RE SS (avian) (SRC), transcript variant 1, OriGene, Cat No. SC125208) or empty vector EV (pCMV6Entry Mammalian Expression Vector, OriGene, Cat No. PS100001). ..

    Variant Assay:

    Article Title: Targeting SRC enhances differentiation and promotes multifaceted cell death mechanisms in recurrent group 3 medulloblastoma.
    Article Snippet: To restore SRC expression in SRC knockout cells, cells were treated with 0.5 μM doxycycline for 24h to induce knock-out before receiving 250μL of plasmid DNA with lipofectamine 3000 and P3000 complex (Invitrogen, Cat No. L3000015) prepared in Opti-MEM media according to manufacturer’s instructions. .. Cells received 0.5μg/mL of SRC (SRC (untagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog AR TI CL E IN P RE SS (avian) (SRC), transcript variant 1, OriGene, Cat No. SC125208) or empty vector EV (pCMV6Entry Mammalian Expression Vector, OriGene, Cat No. PS100001). ..

    Expressing:

    Article Title: Targeting SRC enhances differentiation and promotes multifaceted cell death mechanisms in recurrent group 3 medulloblastoma.
    Article Snippet: To restore SRC expression in SRC knockout cells, cells were treated with 0.5 μM doxycycline for 24h to induce knock-out before receiving 250μL of plasmid DNA with lipofectamine 3000 and P3000 complex (Invitrogen, Cat No. L3000015) prepared in Opti-MEM media according to manufacturer’s instructions. .. Cells received 0.5μg/mL of SRC (SRC (untagged)-Human v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog AR TI CL E IN P RE SS (avian) (SRC), transcript variant 1, OriGene, Cat No. SC125208) or empty vector EV (pCMV6Entry Mammalian Expression Vector, OriGene, Cat No. PS100001). ..

    Sequencing:

    Article Title: A Dimerization Function in the Intrinsically Disordered N-Terminal Region of Src
    Article Snippet: SYF, HEK293T, MDA_MB-468, and MDA-MB-231 cell lines were obtained from the American Type Culture Collection and grown in DMEM/F12 medium containing 10% fetal bovine serum, and 1% (v/v) penicillin –streptomycin solution. .. The coding sequence of human c-Src was amplified from c-Src cDNA clone sc125208 (Origene). .. The cloning strategy was based on the Gateway system (ThermoFisher Scientific).

    Amplification:

    Article Title: A Dimerization Function in the Intrinsically Disordered N-Terminal Region of Src
    Article Snippet: SYF, HEK293T, MDA_MB-468, and MDA-MB-231 cell lines were obtained from the American Type Culture Collection and grown in DMEM/F12 medium containing 10% fetal bovine serum, and 1% (v/v) penicillin –streptomycin solution. .. The coding sequence of human c-Src was amplified from c-Src cDNA clone sc125208 (Origene). .. The cloning strategy was based on the Gateway system (ThermoFisher Scientific).



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