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transcript variant 1  (OriGene)


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    Structured Review

    OriGene transcript variant 1
    Transcript Variant 1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src/SRC+(NM_005417)+Human+Untagged+Clone/pm42031710-117-24-27
    Average 94 stars, based on 3 article reviews
    transcript variant 1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis
    Article Snippet: The N-terminal HA tag was added using the Q5 site-directed mutagenesis kit (NEB) using primers containing the HA tag sequence. .. The short and long forms of SRC-1 was then cloned into the pCDNA3.1(+) vector using KpnI and XhoI restriction sites after PCR amplification of SRC-1 using primers flanking the Origene KpnI and XhoI sites. .. SRC-1 mutant constructs were generated using the Quickchange II XL site-directed mutagenesis kit (Agilent).

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis.
    Article Snippet: The N-terminal HA tag was added using the Q5 site-directed mutagenesis kit (NEB) using primers containing the HA tag sequence. .. The short and long forms of SRC-1 was then cloned into the pCDNA3.1(+) vector using KpnI and XhoI restriction sites after PCR amplification of SRC-1 using primers flanking the Origene KpnI and XhoI sites. .. SRC-1 mutant constructs were generated using the Quickchange II XL site-directed mutagenesis kit (Agilent).

    Polymerase Chain Reaction:

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis
    Article Snippet: The N-terminal HA tag was added using the Q5 site-directed mutagenesis kit (NEB) using primers containing the HA tag sequence. .. The short and long forms of SRC-1 was then cloned into the pCDNA3.1(+) vector using KpnI and XhoI restriction sites after PCR amplification of SRC-1 using primers flanking the Origene KpnI and XhoI sites. .. SRC-1 mutant constructs were generated using the Quickchange II XL site-directed mutagenesis kit (Agilent).

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis.
    Article Snippet: The N-terminal HA tag was added using the Q5 site-directed mutagenesis kit (NEB) using primers containing the HA tag sequence. .. The short and long forms of SRC-1 was then cloned into the pCDNA3.1(+) vector using KpnI and XhoI restriction sites after PCR amplification of SRC-1 using primers flanking the Origene KpnI and XhoI sites. .. SRC-1 mutant constructs were generated using the Quickchange II XL site-directed mutagenesis kit (Agilent).

    Amplification:

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis
    Article Snippet: The N-terminal HA tag was added using the Q5 site-directed mutagenesis kit (NEB) using primers containing the HA tag sequence. .. The short and long forms of SRC-1 was then cloned into the pCDNA3.1(+) vector using KpnI and XhoI restriction sites after PCR amplification of SRC-1 using primers flanking the Origene KpnI and XhoI sites. .. SRC-1 mutant constructs were generated using the Quickchange II XL site-directed mutagenesis kit (Agilent).

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis.
    Article Snippet: The N-terminal HA tag was added using the Q5 site-directed mutagenesis kit (NEB) using primers containing the HA tag sequence. .. The short and long forms of SRC-1 was then cloned into the pCDNA3.1(+) vector using KpnI and XhoI restriction sites after PCR amplification of SRC-1 using primers flanking the Origene KpnI and XhoI sites. .. SRC-1 mutant constructs were generated using the Quickchange II XL site-directed mutagenesis kit (Agilent).

    Construct:

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis
    Article Snippet: .. Generation of SRC-1 constructs and expression plasmids : The long form of SRC-1 containing a C-terminal Flag MYC tag was purchased from Origene (RC224812). .. The short form of SRC-1 was generated using the Q5 site-directed mutagenesis kit (NEB) using primers containing the sequence specific to the short form of SRC-1.

    Expressing:

    Article Title: Steroid receptor coactivator-1 modulates the function of Pomc neurons and energy homeostasis
    Article Snippet: .. Generation of SRC-1 constructs and expression plasmids : The long form of SRC-1 containing a C-terminal Flag MYC tag was purchased from Origene (RC224812). .. The short form of SRC-1 was generated using the Q5 site-directed mutagenesis kit (NEB) using primers containing the sequence specific to the short form of SRC-1.



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