Journal: RSC Advances
Article Title: Probing the effect of the disordered flank regions on amyloid fibril growth and proliferation †
doi: 10.1039/d5ra01654a
Figure Lengend Snippet: Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of capillaries from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
Article Snippet: Capillary flocculation assays were performed using square glass capillaries of 0.4 mm inner diameter and walls of 0.2 mm (VitroCom, Mountain Lakes, NJ, USA).
Techniques: Growth Assay, Flocculation, Fluorescence, Labeling, W/O, Standard Deviation