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VitroCom Inc square glass capillaries of inner diameter and walls of
Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
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Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
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Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
Squared Section Glass Capillary, supplied by VitroCom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
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Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
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Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
Square Glass Capillary, supplied by VitroCom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CM Scientific square glass capillary scientific ryefield (eu)
Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
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CM Scientific hollow square borosilicate glass capillaries id 0.80
Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
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CM Scientific square glass capillary with a cross-section of 0.90 × 2
Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of <t>capillaries</t> from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.
Square Glass Capillary With A Cross Section Of 0.90 × 2, supplied by CM Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of capillaries from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.

Journal: RSC Advances

Article Title: Probing the effect of the disordered flank regions on amyloid fibril growth and proliferation

doi: 10.1039/d5ra01654a

Figure Lengend Snippet: Time course of ThT fibril growth assay and capillary flocculation assay from ESI Video 1 and 2. (A) ThT fluorescence of amyloid fibril growth with and without PK. The dashed line indicates addition of monomer to all samples. PK treatment is notated by color-coded arrows. PK is both added and inhibited before the addition of fresh monomer outside the plate-reader instrument. However, the treatment time, where no data is gathered, is not shown in this graph. ThT is added to samples labeled “w/o ThT” along with additional monomer. Numbering indicates technical repeats. (B) Sample images of capillaries from ESI Video 1 and 2 at time 0 and 180 min. (C) Normalized intensity of ThT-fluorescence throughout the experiment. (D) Change in relative spatial standard deviation of ThT-fluorescence intensity across the capillary as a function of time.

Article Snippet: Capillary flocculation assays were performed using square glass capillaries of 0.4 mm inner diameter and walls of 0.2 mm (VitroCom, Mountain Lakes, NJ, USA).

Techniques: Growth Assay, Flocculation, Fluorescence, Labeling, W/O, Standard Deviation