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spr analysis software  (Interbio Inc)

 
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    Interbio Inc spr analysis software
    Spr Analysis Software, supplied by Interbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spr+analysis/spr+analysis+software/pm37079449-256-27-31
    Average 90 stars, based on 1 article reviews
    spr analysis software - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    SPR Assay:

    Article Title: Light Chain Q166C Mutation Permits One-step Site Specific Conjugation on Monoclonal Antibodies.
    Article Snippet: Engineered cysteines are frequently used for site-specific conjugation in antibody-drug conjugate (ADC) development.. When cysteine-engineered mAbs are produced in the cell culture process, the sulfhydryl groups on the engineered cysteines are mostly in an oxidized form.. The oxidized cysteines require multiple steps (such as reduction, reoxidation, and buffer exchanges) to reactivate for bioconjugation, which complicates the ADC production process and reduces yields.

    Software:

    Article Title: Light Chain Q166C Mutation Permits One-step Site Specific Conjugation on Monoclonal Antibodies.
    Article Snippet: Engineered cysteines are frequently used for site-specific conjugation in antibody-drug conjugate (ADC) development.. When cysteine-engineered mAbs are produced in the cell culture process, the sulfhydryl groups on the engineered cysteines are mostly in an oxidized form.. The oxidized cysteines require multiple steps (such as reduction, reoxidation, and buffer exchanges) to reactivate for bioconjugation, which complicates the ADC production process and reduces yields.



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    Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore T200. (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.

    Journal: Advanced Science

    Article Title: Mitochondrial Localization of Cx26 Promotes Colorectal Cancer Malignancy and Exposes a Fatal Vulnerability to Drug Targeting

    doi: 10.1002/advs.78117

    Figure Lengend Snippet: Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore T200. (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.

    Article Snippet: The Biacore T200 molecular interaction analysis system (GE Healthcare, Uppsala, Sweden) was used to measure the change in response value when the gradient concentration of BTZ solution flowed through the chip, and the kinetic constant of the interaction between small molecules and proteins was obtained.

    Techniques: Expressing, Western Blot, Control, MTT Assay, Docking Assay, Analysis, Incubation, Binding Assay, Activity Assay, shRNA