resource source identifier recombinant human il 15 protein (R&D Systems)

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R&D Systems resource source identifier recombinant human il 15 protein
Resource Source Identifier Recombinant Human Il 15 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15s/Recombinant+Human+IL-15+Protein/pm42013863-236-2-10
Average 95 stars, based on 200 article reviews

resource source identifier recombinant human il 15 protein - by Bioz Stars, 2026-11

95/100 stars

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Recombinant:

Article Title: Ponatinib inhibits LCK and PI3K signaling and promotes CD8 + T stem cell memory cell development.
Article Snippet: The cells were stimulated with Dynabeads Human T-Activator CD3/CD28 at a 1:1 cell-to-bead ratio, lentivirally transduced with the anti-CD19-BBz CAR gene, and treated with either ponatinib (100 nM) or DMSO in RPMI-1640 supplemented with 10% FBS and 4 mM L-glutamine (Thermo Fisher Scientific, #25030-081). .. Recombinant human IL-7 (R&D System, #207-IL) and IL-15 (R&D System, #247- ILB) were added at 10 ng/mL. ..

Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes.
Article Snippet: .. Recombinant IL-15 or IL-21 (R&D Systems) was added at a concentration of 20ng/mL. .. For type I IFN stimulation, CD44hiLy108+ CD8 T cells were sorted and plated at a concentration of 5x104 cells in complete RPMI media + 20ng/mL IL-2 + 1000U/mL universal type I IFN (R&D systems) for 72h.

Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Recombinant Human IL-21 Protein with carrier R&D Systems Cat#8879-IL-010 FTY720 MilliporeSigma Cat344597; CAS:162359-56-0 Deoxyribonucleic acid from calf thymus MilliporeSigma Cat#D4522; CAS:91080-16-9 Reacti-Bind TM DNA Coating Solution Thermo Fisher Cat#17250 DAPI Nucleic Acid Stains Invitrogen Cat#D1306 ProLongTM Gold Antifade Mountant Invitrogen Cat#P36930 Collagenase from Clostridium histolyticum MilliporeSigma Cat#C9263; CAS: 9001-12-1 Renal Epithelial Cell Basal Medium ATCC Cat#PCS-400-030 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10x Genomics Cat#CG000365 Chromium GEM-X Single Cell 5’ Kit 10x Genomics Cat#CG000733 EasySep Mouse CD4+ TCell Isolation Kit STEMCELL Technologies Cat#19852 EasySep Mouse CD8+ TCell Isolation Kit STEMCELL Technologies Cat#19853A EasySepTM Human CD8+ T Cell Isolation Kit STEMCELL Technologies Cat#17953 eBioscienceTM Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521-00 PierceTM Bradford Protein Assay Kit Thermo Scientific Cat#23200 QuantiChromTM Creatinine Assay Kit BioAssay Systems Cat#DICT-500 BD PharmingenTM PE Annexin V Apoptosis Detection Kit I BD Biosciences Cat#559763; RRID:AB_2869265 Deposited data Mouse single cell sequencing data This study Gene Expression Omnibus (GEO) accession number: GSE318514 Human single cell sequencing data Accelerating Medicines Partnership Phase II RA/SLE AMP2 Lupus Kidney Single Cell Mouse strains MRL/MpJ-Faslpr/J The Jackson Laboratory 000485; RRID:IMSR_JAX:000485 NZBWF1/J The Jackson Laboratory 100008; RRID:IMSR_JAX:100008 B6.Cg-Sle1NZM2410/Aeg Yaa/DcrJ The Jackson Laboratory 021569; RRID:IMSR_JAX:021569 B6N.B6-Tg(Nr4a1-EGFP/cre)820Khog/J The Jackson Laboratory 018974; RRID:IMSR_JAX:018974 C57BL/6J The Jackson Laboratory 000664; RRID:IMSR_JAX:000664 MRL/MpJ-Faslpr/J × B6N.B6-Tg(Nr4a1EGFP/cre)820Khog This study N/A MRL/MpJ-Faslpr/J.TCRβ-/- Peng et al.53 10.1084/jem.184.3.1149 Software and algorithms FlowJo v10.8.1 TreeStar FlowJo LLC. .. RRID:SCR_008520 GraphPad Prism v9.5.1 GraphPad Software RRID:SCR_002798 ImageJ imagej.net/ij RRID:SCR_003070 CellRanger v5.0.1 10xgenomics.com/support/software/ cell-ranger RRID:SCR_023221 R version 4.2.0 r-project.org RRID:SCR_001905 chromVAR v1.18 bioconductor.org/packages/chromVAR RRID:SCR_026570 circlize v0.4.16 cran.r-project.org/web/packages/circlize RRID:SCR_002141 cisTopic v0.3.0 github.com/aertslab/cisTopic N/A clusterProfiler v4.6.2 bioconductor.org/packages/clusterProfiler RRID:SCR_016884 Clustree v0.5.0 cran.r-project.org/web/packages/ clustree/index RRID:SCR_016293 (Continued on next page) ll OPEN ACCESS Article e3 Immunity 59, 1–21.e1–e15, June 9, 2026

Article Title: Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.
Article Snippet: To assess IL-7 or IL-15 induced proliferation in vitro, expanded TRM1 and a.a.CD8+ T cells were rested and labelled with CellTrace Violet (Invitrogen) according to the manufacturer's protocol. .. Cells were cultured in X-VIVO medium (LONZA) supplemented with recombinant human IL-15 or IL-7 (both from R&D Systems) at concentrations specified in the figures. ..

Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 T cells arising from tissue-draining lymph nodes
Article Snippet: .. Recombinant IL-15 or IL-21 (R&D Systems) was added at a concentration of 20ng/mL. .. For type I IFN stimulation, CD44 hi Ly108 + CD8 T cells were sorted and plated at a concentration of 5×10 4 cells in complete RPMI media + 20ng/mL IL-2 + 1000U/mL universal type I IFN (R&D systems) for 72h.

Article Title: Integrative omics and phase IIa clinical trial identify TNF as key node in autoimmune hepatitis.
Article Snippet: Frozen TRM1 and a.a. CD8+ T cells were thawed, rested in RPMI at 4°C for 1 hour, and stained with fluorochrome-labelled CD4 and CD8 antibodies (BioLegend), and a viability dye (Thermo Fisher). .. Cells were stimulated with 10 ng/ml recombinant human IL-15 (R&D Systems or PeproTech as specified) or 100 U/ml recombinant human IL-2 in RPMI at 37°C for 15 minutes, fixed with 2% PFA at 37°C for 15 minutes, permeabilized with 100% methanol on ice for 30 minutes, and stained with pSTAT5 antibody (Invitrogen) for 1 hour at room temperature. ..

Concentration Assay:

Immunopeptidomics:

Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination
Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, rhIL-21 (30 ng/ml; R&D Systems, 8879-IL) for 3 days in 48-well plate. rhIL-2 (30 IU/ml; R&D Systems, 202-IL), rhIL-7 (5 ng/ml; R&D Systems, 207-IL), and rhIL-15 (5 ng/ml; R&D Systems, 247-ILB) were supplemented every 2 days to the cocultured cells for a total duration of 14 days before the expanded T cells were used for immunogenicity validation assays. ..

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