Produced:
Purification:
Transfection:Article Title: Kinase condensates enrich ATP and trigger autophosphorylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER InvitrogenTM LipofectamineTM 3000 Transfection Reagent Fisher Scientific Cat#L3000008 HaltTM Protease and Phosphatase Inhibitor Cocktail ThermoFisher Scientific Cat#78440 Paraformaldehyde Fisher Scientific Cat#AA433689M TritonTM X-100 Millipore Sigma Cat#X100 TWEEN® 20 Millipore Sigma Cat#P9416 Saponin Millipore Sigma Cat#47036 Benzamidine Millipore Sigma Cat#B6506 Antipain Millipore Sigma Cat#A6191 Leupeptin Millipore Sigma Cat#L2884 Pepstatin Millipore Sigma Cat#P4265 L-Glutathione Millipore Sigma Cat#G4251 Source 15 Q Anion Exchange Resin Cytiva Cat17094705 Source 15 S Cation Exchange Resin Cytiva Cat#17094401 Amylose resin New England Biolabs Cat#E8021L X-tremeGENETM 9 DNA Transfection Reagent Millipore Sigma Cat#6365779001 Critical commercial assays InvitrogenTM ATP Determination Kit Fisher Scientific Cat#A22066 ADP-GloTM Kinase Assay Promega Cat#V6930 GTPase-GloTM Assay Promega Cat#V7681 Micro BCATM Protein Assay Kit ThermoFisher Scientific Cat#23235 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat#E0554S Deposited data Phospho-MS Data This study Dryad: https://doi.org/10.5061/dryad.j3tx95xwc Experimental models: Cell lines Mouse: MEFs: FAK +/+ ATCC Cat#CRL-2645 Insect: Sf9 Cells Vos laboratory N/A Insect: Sf21 Cells Vos laboratory N/A Insect: HighFiveTM Trichoplusia ni Cells Vos laboratory N/A Recombinant DNA XLone-Puro-EGFP Addgene Cat#140027 XLone-Puro-mEGFP-FAK-WT This study N/A Super PiggyBac Transposase Calo laboratory N/A pFAST-His-TEV-mEGFP-TEV-FAK-WT This study N/A pFAST-His-TEV-mEGFP-TEV-FAK-W266A This study N/A pCMV-mEGFP-FAK-WT Addgene Cat#186148 pCMV-mEGFP-FAK-W266A Addgene Cat#186149 pCMV-mCherry-Paxillin This study N/A pFAST-His-mEGFP-TEV-Abl-WT This study N/A pFAST-His-mEGFP-TEV-Abl-RS This study N/A pCIOX-Mst2 This study N/A pCIOX-mEGFP-Sav1-MBP This study N/A pCIOX-FAK-IDR This study N/A pCIOX-Abl-IDR This study N/A pCIOX-DDX21-C-IDR-WT This study N/A pCIOX-DDX21-C-IDR-RS This study N/A pGEX-PTP1B This study N/A pFAST-His-p130Cas Addgene Cat#186131 pGEX-Paxillin Addgene Cat#186142 pGEX-Nck Addgene Cat#186133 (Continued on next page) 16 Cell Reports ▪▪, 117459, ▪▪, 2026 Article ll OPEN ACCESS .. p53 -/- FAK +/+ mouse embryonic fibroblasts (ATCC CRL-2645) were harvested from E8.0 day old mouse embryo and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), Penicillin/Streptomycin (100 U/mL and 100 mg/mL, respectively) and Glutamax (2 mM) at 37◦C with 5% CO2.
Kinase Assay:Article Title: Kinase condensates enrich ATP and trigger autophosphorylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER InvitrogenTM LipofectamineTM 3000 Transfection Reagent Fisher Scientific Cat#L3000008 HaltTM Protease and Phosphatase Inhibitor Cocktail ThermoFisher Scientific Cat#78440 Paraformaldehyde Fisher Scientific Cat#AA433689M TritonTM X-100 Millipore Sigma Cat#X100 TWEEN® 20 Millipore Sigma Cat#P9416 Saponin Millipore Sigma Cat#47036 Benzamidine Millipore Sigma Cat#B6506 Antipain Millipore Sigma Cat#A6191 Leupeptin Millipore Sigma Cat#L2884 Pepstatin Millipore Sigma Cat#P4265 L-Glutathione Millipore Sigma Cat#G4251 Source 15 Q Anion Exchange Resin Cytiva Cat17094705 Source 15 S Cation Exchange Resin Cytiva Cat#17094401 Amylose resin New England Biolabs Cat#E8021L X-tremeGENETM 9 DNA Transfection Reagent Millipore Sigma Cat#6365779001 Critical commercial assays InvitrogenTM ATP Determination Kit Fisher Scientific Cat#A22066 ADP-GloTM Kinase Assay Promega Cat#V6930 GTPase-GloTM Assay Promega Cat#V7681 Micro BCATM Protein Assay Kit ThermoFisher Scientific Cat#23235 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat#E0554S Deposited data Phospho-MS Data This study Dryad: https://doi.org/10.5061/dryad.j3tx95xwc Experimental models: Cell lines Mouse: MEFs: FAK +/+ ATCC Cat#CRL-2645 Insect: Sf9 Cells Vos laboratory N/A Insect: Sf21 Cells Vos laboratory N/A Insect: HighFiveTM Trichoplusia ni Cells Vos laboratory N/A Recombinant DNA XLone-Puro-EGFP Addgene Cat#140027 XLone-Puro-mEGFP-FAK-WT This study N/A Super PiggyBac Transposase Calo laboratory N/A pFAST-His-TEV-mEGFP-TEV-FAK-WT This study N/A pFAST-His-TEV-mEGFP-TEV-FAK-W266A This study N/A pCMV-mEGFP-FAK-WT Addgene Cat#186148 pCMV-mEGFP-FAK-W266A Addgene Cat#186149 pCMV-mCherry-Paxillin This study N/A pFAST-His-mEGFP-TEV-Abl-WT This study N/A pFAST-His-mEGFP-TEV-Abl-RS This study N/A pCIOX-Mst2 This study N/A pCIOX-mEGFP-Sav1-MBP This study N/A pCIOX-FAK-IDR This study N/A pCIOX-Abl-IDR This study N/A pCIOX-DDX21-C-IDR-WT This study N/A pCIOX-DDX21-C-IDR-RS This study N/A pGEX-PTP1B This study N/A pFAST-His-p130Cas Addgene Cat#186131 pGEX-Paxillin Addgene Cat#186142 pGEX-Nck Addgene Cat#186133 (Continued on next page) 16 Cell Reports ▪▪, 117459, ▪▪, 2026 Article ll OPEN ACCESS .. p53 -/- FAK +/+ mouse embryonic fibroblasts (ATCC CRL-2645) were harvested from E8.0 day old mouse embryo and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), Penicillin/Streptomycin (100 U/mL and 100 mg/mL, respectively) and Glutamax (2 mM) at 37◦C with 5% CO2.
Mutagenesis:Article Title: Kinase condensates enrich ATP and trigger autophosphorylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER InvitrogenTM LipofectamineTM 3000 Transfection Reagent Fisher Scientific Cat#L3000008 HaltTM Protease and Phosphatase Inhibitor Cocktail ThermoFisher Scientific Cat#78440 Paraformaldehyde Fisher Scientific Cat#AA433689M TritonTM X-100 Millipore Sigma Cat#X100 TWEEN® 20 Millipore Sigma Cat#P9416 Saponin Millipore Sigma Cat#47036 Benzamidine Millipore Sigma Cat#B6506 Antipain Millipore Sigma Cat#A6191 Leupeptin Millipore Sigma Cat#L2884 Pepstatin Millipore Sigma Cat#P4265 L-Glutathione Millipore Sigma Cat#G4251 Source 15 Q Anion Exchange Resin Cytiva Cat17094705 Source 15 S Cation Exchange Resin Cytiva Cat#17094401 Amylose resin New England Biolabs Cat#E8021L X-tremeGENETM 9 DNA Transfection Reagent Millipore Sigma Cat#6365779001 Critical commercial assays InvitrogenTM ATP Determination Kit Fisher Scientific Cat#A22066 ADP-GloTM Kinase Assay Promega Cat#V6930 GTPase-GloTM Assay Promega Cat#V7681 Micro BCATM Protein Assay Kit ThermoFisher Scientific Cat#23235 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat#E0554S Deposited data Phospho-MS Data This study Dryad: https://doi.org/10.5061/dryad.j3tx95xwc Experimental models: Cell lines Mouse: MEFs: FAK +/+ ATCC Cat#CRL-2645 Insect: Sf9 Cells Vos laboratory N/A Insect: Sf21 Cells Vos laboratory N/A Insect: HighFiveTM Trichoplusia ni Cells Vos laboratory N/A Recombinant DNA XLone-Puro-EGFP Addgene Cat#140027 XLone-Puro-mEGFP-FAK-WT This study N/A Super PiggyBac Transposase Calo laboratory N/A pFAST-His-TEV-mEGFP-TEV-FAK-WT This study N/A pFAST-His-TEV-mEGFP-TEV-FAK-W266A This study N/A pCMV-mEGFP-FAK-WT Addgene Cat#186148 pCMV-mEGFP-FAK-W266A Addgene Cat#186149 pCMV-mCherry-Paxillin This study N/A pFAST-His-mEGFP-TEV-Abl-WT This study N/A pFAST-His-mEGFP-TEV-Abl-RS This study N/A pCIOX-Mst2 This study N/A pCIOX-mEGFP-Sav1-MBP This study N/A pCIOX-FAK-IDR This study N/A pCIOX-Abl-IDR This study N/A pCIOX-DDX21-C-IDR-WT This study N/A pCIOX-DDX21-C-IDR-RS This study N/A pGEX-PTP1B This study N/A pFAST-His-p130Cas Addgene Cat#186131 pGEX-Paxillin Addgene Cat#186142 pGEX-Nck Addgene Cat#186133 (Continued on next page) 16 Cell Reports ▪▪, 117459, ▪▪, 2026 Article ll OPEN ACCESS .. p53 -/- FAK +/+ mouse embryonic fibroblasts (ATCC CRL-2645) were harvested from E8.0 day old mouse embryo and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), Penicillin/Streptomycin (100 U/mL and 100 mg/mL, respectively) and Glutamax (2 mM) at 37◦C with 5% CO2.
Recombinant:Article Title: Kinase condensates enrich ATP and trigger autophosphorylation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER InvitrogenTM LipofectamineTM 3000 Transfection Reagent Fisher Scientific Cat#L3000008 HaltTM Protease and Phosphatase Inhibitor Cocktail ThermoFisher Scientific Cat#78440 Paraformaldehyde Fisher Scientific Cat#AA433689M TritonTM X-100 Millipore Sigma Cat#X100 TWEEN® 20 Millipore Sigma Cat#P9416 Saponin Millipore Sigma Cat#47036 Benzamidine Millipore Sigma Cat#B6506 Antipain Millipore Sigma Cat#A6191 Leupeptin Millipore Sigma Cat#L2884 Pepstatin Millipore Sigma Cat#P4265 L-Glutathione Millipore Sigma Cat#G4251 Source 15 Q Anion Exchange Resin Cytiva Cat17094705 Source 15 S Cation Exchange Resin Cytiva Cat#17094401 Amylose resin New England Biolabs Cat#E8021L X-tremeGENETM 9 DNA Transfection Reagent Millipore Sigma Cat#6365779001 Critical commercial assays InvitrogenTM ATP Determination Kit Fisher Scientific Cat#A22066 ADP-GloTM Kinase Assay Promega Cat#V6930 GTPase-GloTM Assay Promega Cat#V7681 Micro BCATM Protein Assay Kit ThermoFisher Scientific Cat#23235 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat#E0554S Deposited data Phospho-MS Data This study Dryad: https://doi.org/10.5061/dryad.j3tx95xwc Experimental models: Cell lines Mouse: MEFs: FAK +/+ ATCC Cat#CRL-2645 Insect: Sf9 Cells Vos laboratory N/A Insect: Sf21 Cells Vos laboratory N/A Insect: HighFiveTM Trichoplusia ni Cells Vos laboratory N/A Recombinant DNA XLone-Puro-EGFP Addgene Cat#140027 XLone-Puro-mEGFP-FAK-WT This study N/A Super PiggyBac Transposase Calo laboratory N/A pFAST-His-TEV-mEGFP-TEV-FAK-WT This study N/A pFAST-His-TEV-mEGFP-TEV-FAK-W266A This study N/A pCMV-mEGFP-FAK-WT Addgene Cat#186148 pCMV-mEGFP-FAK-W266A Addgene Cat#186149 pCMV-mCherry-Paxillin This study N/A pFAST-His-mEGFP-TEV-Abl-WT This study N/A pFAST-His-mEGFP-TEV-Abl-RS This study N/A pCIOX-Mst2 This study N/A pCIOX-mEGFP-Sav1-MBP This study N/A pCIOX-FAK-IDR This study N/A pCIOX-Abl-IDR This study N/A pCIOX-DDX21-C-IDR-WT This study N/A pCIOX-DDX21-C-IDR-RS This study N/A pGEX-PTP1B This study N/A pFAST-His-p130Cas Addgene Cat#186131 pGEX-Paxillin Addgene Cat#186142 pGEX-Nck Addgene Cat#186133 (Continued on next page) 16 Cell Reports ▪▪, 117459, ▪▪, 2026 Article ll OPEN ACCESS .. p53 -/- FAK +/+ mouse embryonic fibroblasts (ATCC CRL-2645) were harvested from E8.0 day old mouse embryo and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), Penicillin/Streptomycin (100 U/mL and 100 mg/mL, respectively) and Glutamax (2 mM) at 37◦C with 5% CO2.
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