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primer select program of dnastar lasergene 12 software  (DNASTAR)


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    DNASTAR primer select program of dnastar lasergene 12 software
    Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR <t>Lasergene</t> <t>12</t> software
    Primer Select Program Of Dnastar Lasergene 12 Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+primer+select+program/Lasergene/pmc06508819-88-14-14
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    Images

    1) Product Images from "Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa"

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    Journal: Plant Direct

    doi: 10.1002/pld3.60

    Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software
    Figure Legend Snippet: Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Techniques Used: Sequencing, Amplification, Software

    Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph
    Figure Legend Snippet: Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Techniques Used: Sequencing, Software

    Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software
    Figure Legend Snippet: Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Techniques Used: Sequencing, Generated, Amplification, Clone Assay, Software

    Related Articles

    Sequencing:

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    Article Snippet: .. Sequences from a leafy spurge expressed sequence tag database (Anderson et al., 2007) or obtained by RNA-seq technology were used for designing primer pairs with the Primer-Select program of Lasergene 8 (DNASTAR, Inc., Madison, WI). ..

    Article Title: Missense splice variant (g.20746A>G, p.Ile183Val) of interferon gamma receptor 1 (IFNGR1) coincidental with mycobacterial osteomyelitis - a screen of osteoarticular lesions
    Article Snippet: Peripheral-blood-leukocyte genomic DNA from 19 patients was extracted using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). .. Using primer selection software (Lasergene v8.0, DNASTAR, Madison, Wisconsin, USA), primers were designed for all exons of IFNGR1 :GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NG_007394","term_id":"167830450","term_text":"NG_007394"}} NG_007394 (chromosome 6q23); sequencing was carried out using ABIPRISM 3130, Sequencing Analysis Software v5.4 (Applied Biosystems, Life Technologies Polska, Warsaw, Poland). ..

    Article Title: Identification of some Egyptian leafhopper species (Hemiptera: Cicadellidae) using DNA barcoding
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    Article Title: Development of multiplex PCR assay for concurrent detection of tick borne haemoparasitic infections in bovines.
    Article Snippet: This study describes development and evaluation of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bigemina and Anaplasma marginale infections in bovines.. The assay was developed using parasites specific genomic DNA and three sets of PCR primers targeting the Tams1, 18S rRNA and 16S rRNA genes of T. annulata, B. bigemina and A. marginale, respectively.. Blood samples collected from a total of 461 bovines, suspected for haemoparasitic infections, were examined microscopically to record the status of infection and simultaneously, genomic DNA extracted from these blood samples were utilized for the optimization and validation of multiplex PCR assay.

    RNA Sequencing:

    Article Title: Foliar Glyphosate Treatment Alters Transcript and Hormone Profiles in Crown Buds of Leafy Spurge and Induces Dwarfed and Bushy Phenotypes throughout its Perennial Lifecycle.
    Article Snippet: .. Sequences from a leafy spurge expressed sequence tag database (Anderson et al., 2007) or obtained by RNA-seq technology were used for designing primer pairs with the Primer-Select program of Lasergene 8 (DNASTAR, Inc., Madison, WI). ..

    Software:

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa
    Article Snippet: RT‐PCR was conducted using the Superscript ® One‐Step RT‐PCR protocol with Platinum ® Taq (Invitrogen, Carlsbad, CA, USA). .. Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle. ..

    Article Title: Missense splice variant (g.20746A>G, p.Ile183Val) of interferon gamma receptor 1 (IFNGR1) coincidental with mycobacterial osteomyelitis - a screen of osteoarticular lesions
    Article Snippet: Peripheral-blood-leukocyte genomic DNA from 19 patients was extracted using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). .. Using primer selection software (Lasergene v8.0, DNASTAR, Madison, Wisconsin, USA), primers were designed for all exons of IFNGR1 :GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NG_007394","term_id":"167830450","term_text":"NG_007394"}} NG_007394 (chromosome 6q23); sequencing was carried out using ABIPRISM 3130, Sequencing Analysis Software v5.4 (Applied Biosystems, Life Technologies Polska, Warsaw, Poland). ..

    Article Title: Development of multiplex PCR assay for concurrent detection of tick borne haemoparasitic infections in bovines.
    Article Snippet: This study describes development and evaluation of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bigemina and Anaplasma marginale infections in bovines.. The assay was developed using parasites specific genomic DNA and three sets of PCR primers targeting the Tams1, 18S rRNA and 16S rRNA genes of T. annulata, B. bigemina and A. marginale, respectively.. Blood samples collected from a total of 461 bovines, suspected for haemoparasitic infections, were examined microscopically to record the status of infection and simultaneously, genomic DNA extracted from these blood samples were utilized for the optimization and validation of multiplex PCR assay.

    RNA sequencing:

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa
    Article Snippet: RT‐PCR was conducted using the Superscript ® One‐Step RT‐PCR protocol with Platinum ® Taq (Invitrogen, Carlsbad, CA, USA). .. Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle. ..

    Whole Complete genome sequencing:

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa
    Article Snippet: RT‐PCR was conducted using the Superscript ® One‐Step RT‐PCR protocol with Platinum ® Taq (Invitrogen, Carlsbad, CA, USA). .. Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle. ..

    Selection:

    Article Title: Missense splice variant (g.20746A>G, p.Ile183Val) of interferon gamma receptor 1 (IFNGR1) coincidental with mycobacterial osteomyelitis - a screen of osteoarticular lesions
    Article Snippet: Peripheral-blood-leukocyte genomic DNA from 19 patients was extracted using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). .. Using primer selection software (Lasergene v8.0, DNASTAR, Madison, Wisconsin, USA), primers were designed for all exons of IFNGR1 :GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NG_007394","term_id":"167830450","term_text":"NG_007394"}} NG_007394 (chromosome 6q23); sequencing was carried out using ABIPRISM 3130, Sequencing Analysis Software v5.4 (Applied Biosystems, Life Technologies Polska, Warsaw, Poland). ..



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    Image Search Results


    Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Amplification, Software

    Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Software

    Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Generated, Amplification, Clone Assay, Software