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software primer select program  (DNASTAR)


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    DNASTAR software primer select program
    Software Primer Select Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+primer+select+program/primer+select+software/pm24530311-49-20-22
    Average 90 stars, based on 1 article reviews
    software primer select program - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Software:

    Article Title: Impact of genotype, body weight and sex on the prenatal muscle transcriptome of Iberian pigs
    Article Snippet: .. Primers were designed against each gene, covering different exons, using Primer Select software (DNASTAR, Wisconsin, USA; ). ..

    Article Title: Porcine Epidemic Diarrhea Virus Induces Vero Cell Apoptosis via the p53-PUMA Signaling Pathway
    Article Snippet: .. The above five genes’ primers were designed by using Primer select software in DNAStar software and referring to the principle of designing qRT-PCR primers, as shown in . β-actin was used as the house-keeping gene for internal normalization for qRT-PCR experiments. .. The designed primer sequences of the 6 groups of genes were sent to Sangon Biotech Co., Ltd. (Shanghai, China) for synthesis.

    Article Title: Hypothalamic transcriptome analysis reveals male-specific differences in molecular pathways related to oxidative phosphorylation between Iberian pig genotypes
    Article Snippet: .. Primers were designed using Primer Select Software (DNASTAR, Madison, WI, USA) from the available ENSEMBL sequences. ..

    Article Title: Breed, Diet, and Interaction Effects on Adipose Tissue Transcriptome in Iberian and Duroc Pigs Fed Different Energy Sources
    Article Snippet: .. Primer pairs were designed using Primer Select software (DNASTAR, Madison, WI, USA) from the available GENBANK or ENSEMBL sequences. ..

    Article Title: Identification of Candidate Genes and Regulatory Factors Underlying Intramuscular Fat Content Through Longissimus Dorsi Transcriptome Analyses in Heavy Iberian Pigs
    Article Snippet: .. Primer pairs used for quantification were designed using Primer Select software (DNASTAR, Madison, WI, United States) and/or Primer-Blast from the available GENBANK and/or ENSEMBL sequences, covering different exons in order to assure the amplification of the cDNA. ..

    Article Title: Gene Expression Profile of Mexican Lime ( Citrus aurantifolia ) Trees in Response to Huanglongbing Disease caused by Candidatus Liberibacter asiaticus
    Article Snippet: .. Based on the 10 DEGs sequences from cDNA libraries, specific primers were designed using Primer Select software (DNASTAR Lasergene, Madison, WI, USA). ..

    Article Title: Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
    Article Snippet: .. Primers were designed against each gene, covering different exons to assure the amplification of the cDNA, using Primer Select Software (DNASTAR, Madison, WI, USA) from the available ENSEMBL sequences. ..

    Quantitative RT-PCR:

    Article Title: Porcine Epidemic Diarrhea Virus Induces Vero Cell Apoptosis via the p53-PUMA Signaling Pathway
    Article Snippet: .. The above five genes’ primers were designed by using Primer select software in DNAStar software and referring to the principle of designing qRT-PCR primers, as shown in . β-actin was used as the house-keeping gene for internal normalization for qRT-PCR experiments. .. The designed primer sequences of the 6 groups of genes were sent to Sangon Biotech Co., Ltd. (Shanghai, China) for synthesis.

    Amplification:

    Article Title: Identification of Candidate Genes and Regulatory Factors Underlying Intramuscular Fat Content Through Longissimus Dorsi Transcriptome Analyses in Heavy Iberian Pigs
    Article Snippet: .. Primer pairs used for quantification were designed using Primer Select software (DNASTAR, Madison, WI, United States) and/or Primer-Blast from the available GENBANK and/or ENSEMBL sequences, covering different exons in order to assure the amplification of the cDNA. ..

    Article Title: Muscle Transcriptome Analysis Reveals Molecular Pathways Related to Oxidative Phosphorylation, Antioxidant Defense, Fatness and Growth in Mangalitsa and Moravka Pigs
    Article Snippet: .. Primers were designed against each gene, covering different exons to assure the amplification of the cDNA, using Primer Select Software (DNASTAR, Madison, WI, USA) from the available ENSEMBL sequences. ..

    other:

    Article Title: A PCR-based lateral flow assay for the detection of Turkey ingredient in food products
    Article Snippet: A simple PCR-based lateral flow assay (LFA) was developed to detect turkey ingredient in foods.. We developed this assay based on PCR amplification of turkey DNA, followed by detecting labeled amplicon by LFA.. The results could be observed by the naked eye within 5min following PCR.



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    Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR <t>Lasergene</t> <t>12</t> software
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    Image Search Results


    Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Alignment of FLC chromosomes 8 (Ch08), 13 (Ch13), and 20 (Ch20) sequence obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Coding sequence (CDS) was obtained from Trinity‐assembled RNAseq reads (CO46 and Joelle) or from NCBI (DH55), and consensus sequence (Cons) was obtained from sequencing of PCR‐amplified cDNA. Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Amplification, Software

    Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Pictograph of assembled whole genome sequence (WGS) and Trinity‐assembled RNAseq data (CDS) for FLC located on chromosomes 8 (Csa08), 13 (Csa13), and 20 (Csa20) obtained from the summer (CO46) or winter (Joelle) annual genotypes of Camelina sativa . Sequence alignments were developed using the Megalign Pro application in DNASTAR Lasergene 12 software. The full sequences corresponding to the pictograph can be viewed in Supporting Information Figure . The seven exons of FLC are outlined at the top of figure and lines connect the corresponding location of the MADS‐box (M), Intervening (I), keratin‐like (K), and C‐terminal (C) domains. Primer pairs used to amplify FLC for resequencing (UF2, UR2, DN47065R1, and DN24968F1) are included below the pictograph

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Software

    Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Journal: Plant Direct

    Article Title: Expression of FLOWERING LOCUS C and a frameshift mutation of this gene on chromosome 20 differentiate a summer and winter annual biotype of Camelina sativa

    doi: 10.1002/pld3.60

    Figure Lengend Snippet: Alignment of amino acid sequence for FLC obtained from summer (CO46 and DH55) and winter (Joelle) annual genotypes of Camelina sativa . Sequences specific to chromosomes 8 (Ch8), 13 (Ch13), and 20 (Ch20) from the summer annual DH55 were obtained from NCBI, whereas sequence for the summer and winter annual genotypes was generated from sequence of PCR‐amplified cDNA clones. Alignments were made using the Megalign application in DNASTAR Lasergene 12 software

    Article Snippet: Primers (Supporting Information Table ) were designed using the Primer Select program of DNASTAR Lasergene 12 software (DNASTAR, Inc., Madison, WI, USA) to specifically amplify cDNA based on the assembled camelina RNAseq and WGS of CO46 and Joelle.

    Techniques: Sequencing, Generated, Amplification, Clone Assay, Software