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full-length methods smart-seq2  (fluidigm)


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    Structured Review

    fluidigm full-length methods smart-seq2
    Full Length Methods Smart Seq2, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart-seq2+method/cel+seq2/pm37239891-34-8-12
    Average 90 stars, based on 1 article reviews
    full-length methods smart-seq2 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Uncovering functional lncRNAs by scRNA-seq with ELATUS
    Article Snippet: Specifically, in the introduction we have precise the present state of knowledge in the field and the additional novelty over previous studies (see lines 100-119): 27 “To date, scRNA-seq focused on lncRNAs have been mostly performed using chips or plate-based technologies, such as the Fluidigm C1 microfluidic platform2,3, or the SMART-seq or SMART-seq2 protocols4–7, respectively.

    Article Title: Reliable Identification and Interpretation of Single‐Cell Molecular Heterogeneity and Transcriptional Regulation using Dynamic Ensemble Pruning
    Article Snippet: The cell batches are colorcoded according to their respective batch data names: CelSeq, CelSeq2, Fluidigm C1, and SMART-Seq2. b) Visualizing DEPF for clustering via UMAP.

    Article Title: Advances in single-cell omics and multiomics for high-resolution molecular profiling
    Article Snippet: CEL-seq2 , Fluidigm C1 , O , IVT , 3′ end , Short read , 2016 , .

    Article Title: Single-cell technologies in multiple myeloma: new insights into disease pathogenesis and translational implications
    Article Snippet: , , Smart-seq2 , Switching mechanism at the end of the 5′-end of the RNA transcript sequencing , Full-length coverage across transcripts, high sensitivity , High cost, low throughput , Fluidigm C1 , [ ] .

    Article Title: Decoding the Human Epidermal Complexity at Single-Cell Resolution.
    Article Snippet: Two main library construction methods have been developed: full-length methods (e.g., SMART-seq2, Fluidigm C1) cover the entire transcriptome, while molecular tag-based methods (e.g., 10× Genomics Chromium, MARS-seq, InDrop, Drop-seq) analyze the mRNA 5′ or 3′ ends.

    Article Title: Distribution‐Agnostic Deep Learning Enables Accurate Single‐Cell Data Recovery and Transcriptional Regulation Interpretation
    Article Snippet: The dataset was obtained from multiple sequencing platforms, including CEL‐seq, [ ] CEL‐seq2, [ ] Fluidigm C1, [ ] SMARTer [ , ] inDrop, [ ] and Smart‐seq.

    Single Cell:

    Article Title: DISCERN: deep single-cell expression reconstruction for improved cell clustering and cell subtype and state detection
    Article Snippet: .. Dataset Method Cell Types Publication or Download link pancreas (8569 cells) SMARTSeq2, Fluidigm C1, CelSeq, CelSeq2, inDrops alpha, beta, ductal, acinar, delta, gamma, activated stellate, endothelial, quiescent stellate, macrophage, mast, epsilon, schwann [89] difftec (31 021 cells) 10x Chromium v2, 10x Chromium v3, SMARTSeq2, Seq-Well, inDrops, Drop-seq, CelSeq2 Cytotoxic T cell, CD4+ T cell, CD14+ monocyte, B cell, Natural killer cell, Megakaryocyte, CD16+ monocyte, Dendritic cell, Plasmacytoid dendritic cell, Unassigned [90] covid-lung (56 645 cells) 10X Genomics Chromium Single Cell 5’v1.1 CD8 T, TREG, CD4 CD8 proliferating, B cell, CD4 TCM, TRM1, TR1, CD8 TCM, T senescent, CD8 TEM, TEM17, T antiviral, alveolar MΦ, TRM17, M1, CD4 CD8 stressed TCM, CD4 TSCM, MAIT, Innate like, Neutrophils, doublets, CD4 CD8 lnc rich, aged Neutrophils, M1 HSP+, Mast, DC, M1 Mono-derived, M2 profibrotic, Epithelial, Neutrophil, Macrophage [24] covid-blood (83 709 cells) 10X Genomics Chromium Single Cell 5’v1.1 CD3+ cells [24] citeseq (6592 cells) 10x Genomics Single Cell and CITE-seq B cells, CD4 T cells, NK cells, CD14+ Monocytes, FCGR3A+ Monocytes, CD8 T cells https:// www.ncbi. nlm.nih. gov/geo/ query/ acc. cgi?acc= GSE100866 https: //github. com/ YosefLab/ scVI-data/ raw/ master/ pbmc_ metadata. pickle Table S1: Overview of all single cell and bulk sequencing datasets used in this study continued. ..

    Transmission Electron Microscopy:

    Article Title: DISCERN: deep single-cell expression reconstruction for improved cell clustering and cell subtype and state detection
    Article Snippet: .. Dataset Method Cell Types Publication or Download link pancreas (8569 cells) SMARTSeq2, Fluidigm C1, CelSeq, CelSeq2, inDrops alpha, beta, ductal, acinar, delta, gamma, activated stellate, endothelial, quiescent stellate, macrophage, mast, epsilon, schwann [89] difftec (31 021 cells) 10x Chromium v2, 10x Chromium v3, SMARTSeq2, Seq-Well, inDrops, Drop-seq, CelSeq2 Cytotoxic T cell, CD4+ T cell, CD14+ monocyte, B cell, Natural killer cell, Megakaryocyte, CD16+ monocyte, Dendritic cell, Plasmacytoid dendritic cell, Unassigned [90] covid-lung (56 645 cells) 10X Genomics Chromium Single Cell 5’v1.1 CD8 T, TREG, CD4 CD8 proliferating, B cell, CD4 TCM, TRM1, TR1, CD8 TCM, T senescent, CD8 TEM, TEM17, T antiviral, alveolar MΦ, TRM17, M1, CD4 CD8 stressed TCM, CD4 TSCM, MAIT, Innate like, Neutrophils, doublets, CD4 CD8 lnc rich, aged Neutrophils, M1 HSP+, Mast, DC, M1 Mono-derived, M2 profibrotic, Epithelial, Neutrophil, Macrophage [24] covid-blood (83 709 cells) 10X Genomics Chromium Single Cell 5’v1.1 CD3+ cells [24] citeseq (6592 cells) 10x Genomics Single Cell and CITE-seq B cells, CD4 T cells, NK cells, CD14+ Monocytes, FCGR3A+ Monocytes, CD8 T cells https:// www.ncbi. nlm.nih. gov/geo/ query/ acc. cgi?acc= GSE100866 https: //github. com/ YosefLab/ scVI-data/ raw/ master/ pbmc_ metadata. pickle Table S1: Overview of all single cell and bulk sequencing datasets used in this study continued. ..

    Sequencing:

    Article Title: DISCERN: deep single-cell expression reconstruction for improved cell clustering and cell subtype and state detection
    Article Snippet: .. Dataset Method Cell Types Publication or Download link pancreas (8569 cells) SMARTSeq2, Fluidigm C1, CelSeq, CelSeq2, inDrops alpha, beta, ductal, acinar, delta, gamma, activated stellate, endothelial, quiescent stellate, macrophage, mast, epsilon, schwann [89] difftec (31 021 cells) 10x Chromium v2, 10x Chromium v3, SMARTSeq2, Seq-Well, inDrops, Drop-seq, CelSeq2 Cytotoxic T cell, CD4+ T cell, CD14+ monocyte, B cell, Natural killer cell, Megakaryocyte, CD16+ monocyte, Dendritic cell, Plasmacytoid dendritic cell, Unassigned [90] covid-lung (56 645 cells) 10X Genomics Chromium Single Cell 5’v1.1 CD8 T, TREG, CD4 CD8 proliferating, B cell, CD4 TCM, TRM1, TR1, CD8 TCM, T senescent, CD8 TEM, TEM17, T antiviral, alveolar MΦ, TRM17, M1, CD4 CD8 stressed TCM, CD4 TSCM, MAIT, Innate like, Neutrophils, doublets, CD4 CD8 lnc rich, aged Neutrophils, M1 HSP+, Mast, DC, M1 Mono-derived, M2 profibrotic, Epithelial, Neutrophil, Macrophage [24] covid-blood (83 709 cells) 10X Genomics Chromium Single Cell 5’v1.1 CD3+ cells [24] citeseq (6592 cells) 10x Genomics Single Cell and CITE-seq B cells, CD4 T cells, NK cells, CD14+ Monocytes, FCGR3A+ Monocytes, CD8 T cells https:// www.ncbi. nlm.nih. gov/geo/ query/ acc. cgi?acc= GSE100866 https: //github. com/ YosefLab/ scVI-data/ raw/ master/ pbmc_ metadata. pickle Table S1: Overview of all single cell and bulk sequencing datasets used in this study continued. ..

    FACS:

    Article Title: Adult Human, but Not Rodent, Spermatogonial Stem Cells Retain States with a Foetal-like Signature
    Article Snippet: .. To mitigate batch effects, we excluded from consideration samples which did not use an Illumina sequencer (e.g., [ ]); sequenced too few cells for use with a common informatic workflow, detailed below [ , , ] (<100 cells; these samples are typically from studies employing the whole-transcript but low-throughput Fluidigm C1/SmartSeq or SmartSeq2 methods [ ]); used FACS-sorted, enriched, or otherwise pre-selected cell populations rather than enzymatically digested testes tissue [ , ] (both because the selection process may activate the cells and because the meaningful integration of datasets would not be possible, these samples having biased cell populations); and those where the donor had sex chromosome aneuploidy (e.g., [ ]), testosterone-suppression, non-obstructive azoospermia, or otherwise impaired fertility. ..

    Selection:

    Article Title: Adult Human, but Not Rodent, Spermatogonial Stem Cells Retain States with a Foetal-like Signature
    Article Snippet: .. To mitigate batch effects, we excluded from consideration samples which did not use an Illumina sequencer (e.g., [ ]); sequenced too few cells for use with a common informatic workflow, detailed below [ , , ] (<100 cells; these samples are typically from studies employing the whole-transcript but low-throughput Fluidigm C1/SmartSeq or SmartSeq2 methods [ ]); used FACS-sorted, enriched, or otherwise pre-selected cell populations rather than enzymatically digested testes tissue [ , ] (both because the selection process may activate the cells and because the meaningful integration of datasets would not be possible, these samples having biased cell populations); and those where the donor had sex chromosome aneuploidy (e.g., [ ]), testosterone-suppression, non-obstructive azoospermia, or otherwise impaired fertility. ..



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