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slidehyb #1 buffer  (Thermo Fisher)


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    Thermo Fisher slidehyb #1 buffer
    Slidehyb #1 Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slidehyb+buffer/pmc03514339-294-10-9
    Average 90 stars, based on 1 article reviews
    slidehyb #1 buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: RNA samples were reverse transcribed into cDNA using the Superscript III reverse transcriptase kit (Invitrogen, Carlsbad, USA) and labelled with Cy3 or Cy5 monoreactive dye (GE Healthcare, Amersham, The Netherlands).

    Article Title: Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins
    Article Snippet: RNA quantity and quality were tested with a Nano Drop ND-1000 spectrophotometer (NanoDrop Technologies) and an Agilent Bioanalyzer 2100 (Agilent Technologies Netherlands BV), respectively.

    Article Title: High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis
    Article Snippet: The amino allyl modified cDNA was synthesized with the Superscript III Reverse Transcriptase kit (Invitrogen), purified with Cyscribe GFX purification kit (Amersham Biosciences), labeled with Cy3 and Cy5 dyes and purified again.

    Article Title: Diversity and Strain Specificity of Plant Cell Wall Degrading Enzymes Revealed by the Draft Genome of Ruminococcus flavefaciens FD-1
    Article Snippet: Labeled cDNA was resuspended in 80 μl of preheated (68°C for 15 minutes) SlideHyb #1 (Ambion).

    Labeling:

    Article Title: Identification of potential therapeutic targets in human head & neck squamous cell carcinoma
    Article Snippet: .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides. .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides.

    Article Title: Carbohydrate Availability Regulates Virulence Gene Expression in Streptococcus suis
    Article Snippet: RNA (1 μg) from S. suis samples was labeled using the Cyanine 5 (Cy5) labeling reaction (Amersham Biosciences, Buckinghamshire, UK). .. Co-hybridization with labeled cDNA probes was performed on these oligonucleotide arrays at 42°C for 16 h in Slidehyb#1 (Ambion, Austin, USA). ..

    Hybridization:

    Article Title: Identification of potential therapeutic targets in human head & neck squamous cell carcinoma
    Article Snippet: .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides. .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides.

    Article Title: Sexual differentiation of the zebra finch song system: potential roles for sex chromosome genes
    Article Snippet: .. Slides were hybridized overnight at 42°C using SlideHyb #1 hybridization buffer (Ambion/Applied Biosystems, Foster City, CA), then washed with a series of standard saline citrate solutions, centrifuged to dry, and scanned using an Axon GenePix 4000B (Sunnyvale, CA) microarray scanner. .. Slides were hybridized overnight at 42°C using SlideHyb #1 hybridization buffer (Ambion/Applied Biosystems, Foster City, CA), then washed with a series of standard saline citrate solutions, centrifuged to dry, and scanned using an Axon GenePix 4000B (Sunnyvale, CA) microarray scanner.

    Article Title: The Response of Lactococcus lactis to Membrane Protein Production
    Article Snippet: Indirect Cy-3/Cy-5 labelling of cDNA was performed according to supplier's instructions (Amersham Biosciences, Piscataway, NJ). .. Hybridisation of Cy-labelled cDNA was performed during 16 h at 45°C in a microarray hybridisation incubator ISO20 (Grant Boekel, Cambridgeshire, UK) in Ambion Slidehyb #1 hybridisation buffer (Ambion Biosystems, Foster City, CA). .. SuperAmine glass slides (ArrayIt, Sunnyvale, CA) spotted with duplicates of around 2500 ORF amplicons of L. lactis subsp. cremoris MG1363 were used.

    Microarray:

    Article Title: Identification of potential therapeutic targets in human head & neck squamous cell carcinoma
    Article Snippet: .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides. .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides.

    Article Title: Sexual differentiation of the zebra finch song system: potential roles for sex chromosome genes
    Article Snippet: .. Slides were hybridized overnight at 42°C using SlideHyb #1 hybridization buffer (Ambion/Applied Biosystems, Foster City, CA), then washed with a series of standard saline citrate solutions, centrifuged to dry, and scanned using an Axon GenePix 4000B (Sunnyvale, CA) microarray scanner. .. Slides were hybridized overnight at 42°C using SlideHyb #1 hybridization buffer (Ambion/Applied Biosystems, Foster City, CA), then washed with a series of standard saline citrate solutions, centrifuged to dry, and scanned using an Axon GenePix 4000B (Sunnyvale, CA) microarray scanner.

    Article Title: The Response of Lactococcus lactis to Membrane Protein Production
    Article Snippet: Indirect Cy-3/Cy-5 labelling of cDNA was performed according to supplier's instructions (Amersham Biosciences, Piscataway, NJ). .. Hybridisation of Cy-labelled cDNA was performed during 16 h at 45°C in a microarray hybridisation incubator ISO20 (Grant Boekel, Cambridgeshire, UK) in Ambion Slidehyb #1 hybridisation buffer (Ambion Biosystems, Foster City, CA). .. SuperAmine glass slides (ArrayIt, Sunnyvale, CA) spotted with duplicates of around 2500 ORF amplicons of L. lactis subsp. cremoris MG1363 were used.

    Saline:

    Article Title: Sexual differentiation of the zebra finch song system: potential roles for sex chromosome genes
    Article Snippet: .. Slides were hybridized overnight at 42°C using SlideHyb #1 hybridization buffer (Ambion/Applied Biosystems, Foster City, CA), then washed with a series of standard saline citrate solutions, centrifuged to dry, and scanned using an Axon GenePix 4000B (Sunnyvale, CA) microarray scanner. .. Slides were hybridized overnight at 42°C using SlideHyb #1 hybridization buffer (Ambion/Applied Biosystems, Foster City, CA), then washed with a series of standard saline citrate solutions, centrifuged to dry, and scanned using an Axon GenePix 4000B (Sunnyvale, CA) microarray scanner.



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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Image Search Results


    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) Hybridisation of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.

    Journal: BMC Immunology

    Article Title: The gastrointestinal nematode Trichostrongylus colubriformis down-regulates immune gene expression in migratory cells in afferent lymph

    doi: 10.1186/1471-2172-11-51

    Figure Lengend Snippet: Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) Hybridisation of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.

    Article Snippet: Cy3 and Cy5-labelled cDNA were pooled, concentrated to 10 μl by ethanol precipitation prior to denaturation at 95°C, combining with SlideHyb glass array hybridisation buffer #1 (60 ul, Ambion) and applied to the array.

    Techniques: Infection, Hybridization