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slidehyb buffer 3  (Thermo Fisher)


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    Structured Review

    Thermo Fisher slidehyb buffer 3
    Slidehyb Buffer 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slidehyb+buffer/pmc03373196-112-11-15
    Average 90 stars, based on 1 article reviews
    slidehyb buffer 3 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Prostate-Specific Antigen Modulates the Expression of Genes Involved in Prostate Tumor Growth
    Article Snippet: The probe mixture was denatured at 95°C for 5 minutes, placed on ice for 1 minute, and prepared for hybridization by addition of 110 μl of preheated (65°C) SlideHyb no. 3 buffer (Ambion, Austin, TX).

    Article Title: Array-Comparative Genomic Hybridization Analysis of Primary Endometrial and Ovarian High-grade Neuroendocrine Carcinoma Associated With Adenocarcinoma: Mystery Resolved?
    Article Snippet: High-grade neuroendocrine carcinomas (NECs) of the uterine corpus and ovary are very rare tumors, and whenever diagnosed, they are usually associated with epithelial carcinoma.. Because of their rarity, the molecular characteristics of these tumors are still unknown.. To shed some light, we studied the genetic abnormalities of each of the 2 components, NEC and adenocarcinoma, in 4 cases arising in the ovary and the uterine corpus using array-comparative genomic hybridization.

    Article Title: Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis
    Article Snippet: The labeled cDNA mixture was eluted in 110 μ L of SlideHyb buffer no. 3 (Ambion, Austin, TX) and heated for 5 minutes at 70°C prior to hybridization.

    Article Title: Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes
    Article Snippet: The probe mixture was denatured at 95 °C for 5 min, placed on ice for 1 min, and prepared for hybridization by the addition of 110 μl of preheated (65 °C) SlideHyb No. 3 buffer (Ambion, Austin, TX).

    Article Title: Array-based Comparative Genomic Hybridization (aCGH) Analysis is a Useful Tool for Distinguishing Primary Pulmonary From Metastatic Neuroendocrine Carcinoma to the Lung
    Article Snippet: Distinguishing primary from metastatic disease is vital for patient prognosis and care.. This becomes a difficult task for both clinicians and pathologists when the clinical data, morphology, and immunohistochemistry (IHC) are not congruent.. Array-based comparative genomic hybridization (aCGH) is a fairly recent technique that enables the analysis of whole tumor genome for discreet genetic abnormalities.

    Microarray:

    Article Title: Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts.
    Article Snippet: Suchyta, Steven P., Sue Sipkovsky, Robert G. Halgren, Rachael Kruska, Michael Elftman, Miriam Weber-Nielsen, Michael J. Vandehaar, Lan Xiao, Robert J. Tempelman, and Paul M. Coussens.. Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts.. Physiol Genomics 16: 8–18, 2004.

    Article Title: Development of a porcine brain cDNA library, EST database, and microarray resource.
    Article Snippet: Nobis, William, Xiaoning Ren, Steven P. Suchyta, Thomas R. Suchyta, Adroaldo J. Zanella, and Paul M. Coussens.. Development of a porcine brain cDNA library, EST database, and microarray resource.. Physiol Genomics 16: 153–159, 2003.

    Hybridization:

    Article Title: Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts.
    Article Snippet: Suchyta, Steven P., Sue Sipkovsky, Robert G. Halgren, Rachael Kruska, Michael Elftman, Miriam Weber-Nielsen, Michael J. Vandehaar, Lan Xiao, Robert J. Tempelman, and Paul M. Coussens.. Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts.. Physiol Genomics 16: 8–18, 2004.

    Incubation:

    Article Title: Development of a porcine brain cDNA library, EST database, and microarray resource.
    Article Snippet: Nobis, William, Xiaoning Ren, Steven P. Suchyta, Thomas R. Suchyta, Adroaldo J. Zanella, and Paul M. Coussens.. Development of a porcine brain cDNA library, EST database, and microarray resource.. Physiol Genomics 16: 153–159, 2003.



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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Thermo Fisher slidehyb buffer 1
    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) <t>Hybridisation</t> of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.
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    Image Search Results


    Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) Hybridisation of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.

    Journal: BMC Immunology

    Article Title: The gastrointestinal nematode Trichostrongylus colubriformis down-regulates immune gene expression in migratory cells in afferent lymph

    doi: 10.1186/1471-2172-11-51

    Figure Lengend Snippet: Experimental Design . (A) Truncated Immunising infection and Challenge protocol. Daily afferent lymph samples were collected over a 13 week period. RNA was pooled (sample pools) after extraction from afferent lymph samples from weeks labelled PI (Pre-infection, week 0), I1 (Immunising infection 1, week 1), I2 (Immunising infection 2, week 4), I3 (Immunising infection 3, week 7), C1 (Challenge infection, week 10), C2 (Challenge infection, week 11) and C3 (Challenge infection, week 12). N; number of animals in each sample pool, solid arrows show infection with 50,000 T. colubriformis ( Tc ) and dashed arrows show drench treatment. (B) Hybridisation of RNA pools. Solid lines show the comparisons made; solid arrows show nematode infection and dashed arrows show drench treatments.

    Article Snippet: Cy3 and Cy5-labelled cDNA were pooled, concentrated to 10 μl by ethanol precipitation prior to denaturation at 95°C, combining with SlideHyb glass array hybridisation buffer #1 (60 ul, Ambion) and applied to the array.

    Techniques: Infection, Hybridization