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on column dnase digestion  (Qiagen)


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  • 98

    Structured Review

    Qiagen on column dnase digestion
    On Column Dnase Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 2019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/set/RNase-Free+DNase+Set/pmc10076021-348-16-19
    Average 98 stars, based on 2019 article reviews
    on column dnase digestion - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: Octopamine receptors at a glance: from expression and anatomical maps to their role in development and behavior in the Drosophila melanogaster larva
    Article Snippet: Sequenced PCR products were compared to the core nucleotide database and nucleotide collection by National Cen-ter for Biotechnology Information ( ; https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=Blast-Search&LINK_LOC=blasthome ). .. Total RNA from L3 larvae was isolated using QIAzol (Qiagen, 79306) with following DNA digestion using RNase-Free DNase Set (Qiagen, 79256) and purification using RNeasy Mini Kit (Qiagen, 74104), all steps were performed according to the manufac-turers protocol. .. 250 μg RNA was reverse transcribed using SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen, 11752050).

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: .. Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System.

    Article Title: Pregnancy negates thyroid hormone-induced pyrexia.
    Article Snippet: Band intensities were quantified using Image Lab software (version 6.1, Bio-Rad, RRID: 164 SCR_014210) and normalized to total protein load. .. 165 Quantitative Real-Time PCR (qPCR) 166 RNA was extracted from snap-frozen tissues using tissue-specific RNA isolation kits from QIAGEN 167 including a DNase step (QIAGEN, #79256, Germany) following the manufacturer’s instructions. .. Liver, 168 muscle, and heart tissues were ground on liquid nitrogen prior to RNA isolation. iBAT and iWAT were 169 isolated using the RNeasy Lipid Tissue Mini Kit (QIAGEN, #74804, Germany), muscle and heart with the 170 RNeasy Fibrous Tissue Mini Kit (QIAGEN, #74704, Germany), and the liver with RNeasy Mini Kit (QIAGEN, 171 #74104, Germany).

    Purification:

    Article Title: Octopamine receptors at a glance: from expression and anatomical maps to their role in development and behavior in the Drosophila melanogaster larva
    Article Snippet: Sequenced PCR products were compared to the core nucleotide database and nucleotide collection by National Cen-ter for Biotechnology Information ( ; https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=Blast-Search&LINK_LOC=blasthome ). .. Total RNA from L3 larvae was isolated using QIAzol (Qiagen, 79306) with following DNA digestion using RNase-Free DNase Set (Qiagen, 79256) and purification using RNeasy Mini Kit (Qiagen, 74104), all steps were performed according to the manufac-turers protocol. .. 250 μg RNA was reverse transcribed using SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen, 11752050).

    Article Title: Dual Targeting of DNA and EGFR by ZYH005 Induces DNA Damage and Mitotic Catastrophe in Glioblastoma
    Article Snippet: Total RNA was extracted from U87‐MG cells using TRIzol reagent (Cat# 15596‐018, Life Technologies, Carlsbad, CA, USA). .. RNA purification was performed using the NucleoSpin RNA Clean‐up XS kit (Cat#740903, MN, Germany) and the RNase‐Free DNase Set (Cat79254, QIAGEN, GmbH, Germany). .. According to the Affymetrix technical manual, the double‐stranded cDNA was hybridized with biotin‐labeled fragmented cRNA.

    Article Title: The good, the bad, and the stable: Reference genes for preclinical biodistribution studies
    Article Snippet: .. To remove contaminating genomic DNA, an on-column DNA digest was performed during the RNA purification process, following the manufacturer’s recommended procedure (QIAGEN, RNase-Free DNase Set, Cat. no. 79256). .. The concentration and purity of the RNA were assessed using a Nanodrop 8 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: The Good, the Bad and the Stable: Selecting Housekeeping Genes for dPCR-based RNA Quantification in NHP and Mouse Preclinical Studies
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Gene Expression:

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: .. Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System.

    Real-time Polymerase Chain Reaction:

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: .. Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System.

    Article Title: Pregnancy negates thyroid hormone-induced pyrexia.
    Article Snippet: Band intensities were quantified using Image Lab software (version 6.1, Bio-Rad, RRID: 164 SCR_014210) and normalized to total protein load. .. 165 Quantitative Real-Time PCR (qPCR) 166 RNA was extracted from snap-frozen tissues using tissue-specific RNA isolation kits from QIAGEN 167 including a DNase step (QIAGEN, #79256, Germany) following the manufacturer’s instructions. .. Liver, 168 muscle, and heart tissues were ground on liquid nitrogen prior to RNA isolation. iBAT and iWAT were 169 isolated using the RNeasy Lipid Tissue Mini Kit (QIAGEN, #74804, Germany), muscle and heart with the 170 RNeasy Fibrous Tissue Mini Kit (QIAGEN, #74704, Germany), and the liver with RNeasy Mini Kit (QIAGEN, 171 #74104, Germany).



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    Image Search Results


    Journal: iScience

    Article Title: Genome-wide mapping of the Galleria mellonella transcription start sites during fungal infection and treatment

    doi: 10.1016/j.isci.2026.117675

    Figure Lengend Snippet:

    Article Snippet: MGIEasy RNA Directional Library Prep Set , MGI Tech , 1000006386.

    Techniques: Suction Filtration, Saline, Sensitivity, Qubit, Sequencing, Genome Wide, Infection, Software