dnase i (Qiagen)

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Qiagen dnase i
Dnase I, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26899 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/set/RNase-Free+DNase+Set/pm42894714-891-15-17
Average 99 stars, based on 26899 article reviews

dnase i - by Bioz Stars, 2026-10

99/100 stars

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Activity Assay:

Article Title: Extracellular DNA concentrations in various aetiologies of acute kidney injury
Article Snippet: One microliter of each sample was analysed in 1% agarose gel (2 mM MgCl2, 2 mM CaCl2, 20 mM Tris–HCl pH 7.5, 0.035 mg/mL DNA isolated from porcine livers). .. The calibration curve was made of DNase I standard of known DNase activity, with serial two-fold dilutions in the RDD buffer presented in the set (RNase-free DNase set, Qiagen, Hilden, Germany). ..

Article Title: Extracellular DNA Correlates with Intestinal Inflammation in Chemically Induced Colitis in Mice
Article Snippet: One microliter of the samples were pipetted into the holes of 1% agarose gel (20 mM Tris-HCl pH 7.5, 2 mM CaCl 2 , 2 mM MgCl 2 and DNA (5 mg/mL) isolated from chicken livers) and incubated overnight (16–20 h) at 37 °C in the dark and visualized by iBOX (Vision works LP Analysis Software, UVP, Upland, CA, USA). .. The radius of the circles formed was measured using ImageJ software (NIH, Bethesda, Maryland, MD, USA) and compared to the calibration curve from the DNase standards which were twofold dilutions of DNase I in RDD buffer presented in the set (RNase-free DNase set, Qiagen, Hilden, Germany) with known DNase activity. ..

Derivative Assay:

Article Title: Chromatin Accessibility and Gene Expression Vary Between a New and Evolved Autopolyploid of Arabidopsis arenosa.
Article Snippet: .. Due to insufficient RNA derived from 9 the third set (using the Qiagen RNeasy Plant Mini kit), petals were once again collected (from 10 10 healthy open flowers per biological replicate) at 9 weeks after the onset of flowering and subjected 11 to RNA extraction using the Spectrum Plant Total RNA Kit (Sigma), which resulted in higher RNA 12 yield. ..

Article Title: Chromatin Accessibility and Gene Expression Vary Between a New and Evolved Autopolyploid of Arabidopsis arenosa
Article Snippet: .. Due to insufficient RNA derived from the third set (using the Qiagen RNeasy Plant Mini kit), petals were once again collected (from ten healthy open flowers per biological replicate) at 9 weeks after the onset of flowering and subjected to RNA extraction using the Spectrum Plant Total RNA Kit (Sigma), which resulted in higher RNA yield. .. The apical meristem of 14-d-old seedlings was treated with 0.05% colchicine (Sigma C9754) diluted in sterile water with 0.05% Silwet-77 (Anawa 30630216).

RNA Extraction:

Purification:

Article Title: iMSC exosome delivers hsa-mir-125b-5p and strengthens acidosis resilience through suppression of ASIC1 protein in cerebral ischemia-reperfusion
Article Snippet: The total mRNA was extracted with RNeasy Micro Kit (74004 Qiagen) following the manufacturer’s instructions for library construction. .. Qualified total RNA was purified by RNAClean XP kit (A6398.7, Beckman Coulter, Inc) and RNAase-Free Set (79254, QIAGEN). .. The sequencing library was prepared with VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (NR604-02, Vazyme) according to the manufacturer’s instructions.

Article Title: iMSC exosome delivers hsa-mir-125b-5p and strengthens acidosis resilience through suppression of Asic1 protein in cerebral ischemia-reperfusion.
Article Snippet: RNA-seq experiment and library preparation The total mRNA was extracted with RNeasy Micro Kit (74004 Qiagen) following the manufacturer’s instructions for library construction. .. Qualified total RNA was purified by RNAClean XP kit (A6398.7, Beckman Coulter, Inc.) and RNAase-Free Set (79254, QIAGEN, Germany). .. The sequencing library was prepared with VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® (NR604–02, Vazyme) according to the manufacturer’s instructions.

Lysis:

Article Title: Polyphenolic Secondary Metabolites Synergize the Activity of Commercial Antibiotics against Clinical Isolates of β-Lactamase-producing Klebsiella pneumoniae.
Article Snippet: Emergence of worldwide antimicrobial resistance prompted us to study the resistance modifying potential of plant-derived dietary polyphenols, mainly caffeic acid, ellagic acid, epigallocatechin-3-gallate (EGCG) and quercetin.. These compounds were studied in logical combination with clinically significant antibiotics (ciprofloxacin/ gentamicin/tetracycline) against Klebsiella pneumoniae, after conducting phenotypic screening of a large number of clinical isolates and selecting the relevant strains possessing extended-spectrum β-lactamase (ESBL) and K. pneumoniae carbapenemase (KPC)-type carbapenemase enzymes only.. The study demonstrated that EGCG and caffeic acid could synergize the activity of tested antibiotics within a major population of β-lactamaseproducingK. pneumoniae.

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