Review



digestion buffer containing trypsin (modified sequencing grade)  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Promega digestion buffer containing trypsin (modified sequencing grade)
    Digestion Buffer Containing Trypsin (Modified Sequencing Grade), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/trypsin+buffer/pm21668887-58-12-16
    Average 90 stars, based on 1 article reviews
    digestion buffer containing trypsin (modified sequencing grade) - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Phospho-proteomics:

    Article Title: C9ORF72 poly-PR disrupts expression of ALS/FTD-implicated STMN2 through SRSF7.
    Article Snippet: S-trap mini columns were used (Protifi, NY) according to manufacturer protocol. .. Briefly, samples were resolubilized in 5% SDS for reduction and alkylation, further digested in buffer trypsin (Promega) for 5 h. The digested samples were enriched by HighSelectTM TiO2 Phosphopeptide Enrichment Kit (ThermoFisher) according to the vendor’s instructions. .. Enriched phosphopeptides were labeled with TMT16plexPRO (Thermo-Fisher) according to manufacturer protocol.

    Dissolution:

    Article Title: A Method for Identification of Biotype-Specific Salivary Effector Candidates of Aphid.
    Article Snippet: .. The protein suspensions were digested with 40 μL of trypsin buffer (Promega, Madison, WI, USA) at 37 ◦C for 18 h. The filter unit was transferred to a new tube, added 40 μL dissolution buffer, and centrifuged at 14,000× g at 4 ◦C for 30 min. ..

    Article Title: Evaluation and Characterization of Acute respiratory distress syndrome in tree shrews through TMT proteomic method.
    Article Snippet: Next, 100 μL of dissolution buffer (Applied Biosystems, Foster City, USA) was added to each filter, centrifuged at 14,000 × g for 10 min, and repeated three times. .. Then, trypsin buffer (52 μL, Promega, USA) was used to digest protein suspensions at 37°C for 18 h. At last, the filter unit was transferred to a new tube, and 40 μL of dissolution buffer was added. ..

    Article Title: A Method for Identification of Biotype-Specific Salivary Effector Candidates of Aphid
    Article Snippet: .. The protein suspensions were digested with 40 μL of trypsin buffer (Promega, Madison, WI, USA) at 37 °C for 18 h. The filter unit was transferred to a new tube, added 40 μL dissolution buffer, and centrifuged at 14,000× g at 4 °C for 30 min. ..

    Incubation:

    Article Title: In-depth proteomic profiling identifies potentiation of the LPS response by 7-ketocholesterol
    Article Snippet: .. Digestion buffer (50 mM ammonium bicarbonate containing trypsin (Promega) at a 1:20 ratio (trypsin:protein)) was added to the top of the column and incubated at 47 °C without shaking for 2 h. Peptides were then eluted by addition and sequential centrifugation at 4000 g of 80 μl of digestion buffer, 80 μL of 0.2 % formic acid, and 80 μL of 50 % acetonitrile containing 0.2 % formic acid. .. Eluted peptides were dried down using a SpeedVac (GeneVac), then reconstituted in 1 % formic acid by incubating on a ThermoMixer at 30 °C for 1 h with shaking at 1000 rpm.

    Centrifugation:

    Article Title: In-depth proteomic profiling identifies potentiation of the LPS response by 7-ketocholesterol
    Article Snippet: .. Digestion buffer (50 mM ammonium bicarbonate containing trypsin (Promega) at a 1:20 ratio (trypsin:protein)) was added to the top of the column and incubated at 47 °C without shaking for 2 h. Peptides were then eluted by addition and sequential centrifugation at 4000 g of 80 μl of digestion buffer, 80 μL of 0.2 % formic acid, and 80 μL of 50 % acetonitrile containing 0.2 % formic acid. .. Eluted peptides were dried down using a SpeedVac (GeneVac), then reconstituted in 1 % formic acid by incubating on a ThermoMixer at 30 °C for 1 h with shaking at 1000 rpm.



    Similar Products

    trypsin (sequencing grade, modified, including ac ution buffer, promega, madison, usa)  (Promega)
    90
    Promega trypsin (sequencing grade, modified, including ac ution buffer, promega, madison, usa)
    Trypsin (Sequencing Grade, Modified, Including Ac Ution Buffer, Promega, Madison, Usa), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/trypsin+promega/10__1016_slash_j__foodchem__2025__144383-35-19-29
    Average 90 stars, based on 1 article reviews
    trypsin (sequencing grade, modified, including ac ution buffer, promega, madison, usa) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    sequencing grade modified trypsin buffer  (Promega)
    90
    Promega sequencing grade modified trypsin buffer
    Sequencing Grade Modified Trypsin Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/sequencing+grade+modified+trypsin+with+resuspension+buffer/pmc11984660-110-33-38
    Average 90 stars, based on 1 article reviews
    sequencing grade modified trypsin buffer - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    sequencing grade modified trypsin with resuspension buffer  (Promega)
    90
    Promega sequencing grade modified trypsin with resuspension buffer
    Sequencing Grade Modified Trypsin With Resuspension Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/sequencing+grade+modified+trypsin+with+resuspension+buffer/pm36162445-66-5-10
    Average 90 stars, based on 1 article reviews
    sequencing grade modified trypsin with resuspension buffer - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    sequencing grade modified trypsin in trypsin resuspension buffer  (Promega)
    90
    Promega sequencing grade modified trypsin in trypsin resuspension buffer
    Sequencing Grade Modified Trypsin In Trypsin Resuspension Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/sequencing+grade+modified+trypsin+with+resuspension+buffer/pmc07679414-331-8-11
    Average 90 stars, based on 1 article reviews
    sequencing grade modified trypsin in trypsin resuspension buffer - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    sequencing grade modified trypsin buffer  (Thermo Fisher)
    99
    Thermo Fisher sequencing grade modified trypsin buffer
    Sequencing Grade Modified Trypsin Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/Trypsin/10__1016_slash_j__indcrop__2018__12__057-81-8-32
    Average 99 stars, based on 1 article reviews
    sequencing grade modified trypsin buffer - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    digestion buffer (50 nh4hco3, 12.5 ng/μl of modified porcine trypsin, sequencing grade, promega)  (Promega)
    90
    Promega digestion buffer (50 nh4hco3, 12.5 ng/μl of modified porcine trypsin, sequencing grade, promega)
    Digestion Buffer (50 Nh4hco3, 12.5 Ng/μl Of Modified Porcine Trypsin, Sequencing Grade, Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/trypsin+promega/pm22799892-62-6-19
    Average 90 stars, based on 1 article reviews
    digestion buffer (50 nh4hco3, 12.5 ng/μl of modified porcine trypsin, sequencing grade, promega) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    digestion buffer containing trypsin (modified sequencing grade)  (Promega)
    90
    Promega digestion buffer containing trypsin (modified sequencing grade)
    Digestion Buffer Containing Trypsin (Modified Sequencing Grade), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/trypsin+buffer/pm21668887-58-12-16
    Average 90 stars, based on 1 article reviews
    digestion buffer containing trypsin (modified sequencing grade) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results