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digestion buffer (50 nh4hco3, 12.5 ng/μl of modified porcine trypsin, sequencing grade, promega)  (Promega)

 
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    Structured Review

    Promega digestion buffer (50 nh4hco3, 12.5 ng/μl of modified porcine trypsin, sequencing grade, promega)
    Digestion Buffer (50 Nh4hco3, 12.5 Ng/μl Of Modified Porcine Trypsin, Sequencing Grade, Promega), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+grade+modified+trypsin+buffer/trypsin+promega/pm22799892-62-6-19
    Average 90 stars, based on 1 article reviews
    digestion buffer (50 nh4hco3, 12.5 ng/μl of modified porcine trypsin, sequencing grade, promega) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Proteomic analysis of serum samples after cardiac arrest: Rationale and design of a TTM-trial substudy
    Article Snippet: The samples will then be further digested using 100 μl trypsin (0.02 μg/μl, sequencing-grade modified porcine trypsin, Promega) for 16 h at room temperature.

    Article Title: Integrative multi-omics characterization of 12 syngeneic mouse models
    Article Snippet: Trypsin , Promega , V5280.

    Article Title: In vivo protein half-life analysis identifies the SREBF1-SLC27a5 axis governs antioxidant response in preclinical alcoholic rat model
    Article Snippet: A total of 50 μg equivalent proteins from plasma and cell lysate samples were measured using Bradford protein estimation kit (Pierce Bradford: 23236), were subjected to reduction with 10 mM DTT at 60 °C for 1Hr, alkylation with 10 mM Iodoacetamide (IAA) in the dark at room temperature, and digestion for 24 h at 37 °C using trypsin (Promega: V5280).

    Article Title: Isolation, biochemical characterization, and primary structure and active site determination of Dioscorea opposita ('Nagaimo') oligopeptidase B.
    Article Snippet: Sequencing-grade modified trypsin and RNA isolation kits, and ExTaq were obtained from Promega (Tokyo, Japan) and Takara Bio (Shiga, Japan), respectively.

    Article Title: Changes in physical properties and characteristic peptides in porcine gelatin-containing gummy candies at different storage stages during shelf-life
    Article Snippet: The physical properties and gelatin identification of gummy candies could be affected in the storage process by acid hydrolysis and glycation reaction.. In this work, the physical properties including texture, color, appearance and molecular weight distribution were evaluated in the porcine gelatin-containing gummy candies that were stored under the accelerated model at different storage stages.. In addition, the characteristic peptides of porcine gelatin were identified by high performance liquid chromatography and linear ion trap/Orbitrap MS/MS (HPLCLTQ/Orbitrap MS/MS) to investigate their changes.

    Article Title: The mitochondrion of Plasmodium falciparum generates essential acetyl-CoA for protein acetylation
    Article Snippet: Samples were digested by adding 100 μL trypsin solution (10 mM TEAB, pH 7.5, 2 μg Promega trypsin #V5111) and incubated overnight at 37 °C.

    Article Title: Isolation, biochemical characterization, and primary structure and active site determination of Dioscorea opposita (‘Nagaimo’) oligopeptidase B
    Article Snippet: Sequencing-grade modified trypsin and RNA isolation kits, and Ex-Taq were obtained from Promega (Tokyo, Japan) and Takara Bio (Shiga, Japan), respectively.

    Sequencing:

    Article Title: Artemisinin synergizes with CCCP in autophagic cell death induction via ER stress in uveal melanoma
    Article Snippet: .. Then the protein precipitates were digested by sequencing grade modified trypsin (Promega) at a protein-to-enzyme of 50:1 at 37°C overnight and then collected by centrifuge for 20 min at 14,000g. ..

    Modification:

    Article Title: Artemisinin synergizes with CCCP in autophagic cell death induction via ER stress in uveal melanoma
    Article Snippet: .. Then the protein precipitates were digested by sequencing grade modified trypsin (Promega) at a protein-to-enzyme of 50:1 at 37°C overnight and then collected by centrifuge for 20 min at 14,000g. ..



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