seqsphere+ software (Ridom GmbH)
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Seqsphere+ Software, supplied by Ridom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/seqsphere+++software/seqsphere/pmc12278488-119-12-15
Average 90 stars, based on 1 article reviews
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1) Product Images from "Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering"
Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering
Journal: BMC Microbiology
doi: 10.1186/s12866-025-04152-8
Figure Legend Snippet: Population structure of the global Rhodococcus equi dataset including 233 genomes from 18 countries. Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Ridom Seqsphere + v9.0.1 ( https://www.ridom.de/seqsphere/cgmlst/ ) and converted to a neighbor-joining phylogeny using the R package ape . The cgMLST phylogeny was rooted at the mid-point and visualized using iTOL . The year and country of isolation are shown in the inner and second ring from the centre respectively. Source attribution of the different isolates is shown in the third ring. Presence/absence of the virulence-associated plasmid and the various plasmid categories are color-coded in the outer ring. The scale bar expresses the average number of nucleotide substitutions per site
Techniques Used: Construct, Generated, Isolation, Plasmid Preparation
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Construct:Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022) Article Snippet: MLST and cgMLST were carried out using established Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections. Article Snippet: Total DNA from the isolates was extracted via a total DNA kit (Zymo) and quantified via a Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background. Article Snippet: DNA extraction was performed using the QIAsymphony DSP Virus/Pathogen Midi kit (Qiagen GmbH, Hilden, Germany) with the QIAsymphony system (Qiagen) according to the manufacturer's instructions, with RNase treatment and elution in Tris–HCl (pH 8). Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management. Article Snippet: The resulting genome sequences were analysed using Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine. Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 ( Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Core-genome MLST (cgMLST) analysis for 286 Generated:Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022) Article Snippet: MLST and cgMLST were carried out using established Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections. Article Snippet: Total DNA from the isolates was extracted via a total DNA kit (Zymo) and quantified via a Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background. Article Snippet: DNA extraction was performed using the QIAsymphony DSP Virus/Pathogen Midi kit (Qiagen GmbH, Hilden, Germany) with the QIAsymphony system (Qiagen) according to the manufacturer's instructions, with RNase treatment and elution in Tris–HCl (pH 8). Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management. Article Snippet: The resulting genome sequences were analysed using Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine. Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 ( Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Core-genome MLST (cgMLST) analysis for 286 Isolation:Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022) Article Snippet: MLST and cgMLST were carried out using established Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections. Article Snippet: Total DNA from the isolates was extracted via a total DNA kit (Zymo) and quantified via a Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background. Article Snippet: DNA extraction was performed using the QIAsymphony DSP Virus/Pathogen Midi kit (Qiagen GmbH, Hilden, Germany) with the QIAsymphony system (Qiagen) according to the manufacturer's instructions, with RNase treatment and elution in Tris–HCl (pH 8). Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management. Article Snippet: The resulting genome sequences were analysed using Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine. Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 ( Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Core-genome MLST (cgMLST) analysis for 286 Plasmid Preparation:Article Title: Genomic Epidemiology of Enterococcus faecium Bloodstream Infections During a VanB-type VRE Peak Reveals an Oligoclonal Scenario: An Observational Study at a German University Hospital (2017–2022) Article Snippet: MLST and cgMLST were carried out using established Article Title: Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections. Article Snippet: Total DNA from the isolates was extracted via a total DNA kit (Zymo) and quantified via a Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Article Title: Staphylococcus aureus From Prosthetic Joint Infections and Blood Cultures Display the Same Genetic Background. Article Snippet: DNA extraction was performed using the QIAsymphony DSP Virus/Pathogen Midi kit (Qiagen GmbH, Hilden, Germany) with the QIAsymphony system (Qiagen) according to the manufacturer's instructions, with RNase treatment and elution in Tris–HCl (pH 8). Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Tree constructed using ad hoc core-genome MLST (cgMLST) scheme containing 2’737 genes generated in Article Title: Interpreting whole-genome sequencing data during neonatal Klebsiella oxytoca complex outbreak management. Article Snippet: The resulting genome sequences were analysed using Article Title: High genetic relatedness between multidrug resistant bacteria before and after the 2022 invasion of Ukraine. Article Snippet: First, core genome MLST was performed in SeqSphere + Software v 7.7.2 ( Article Title: Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering Article Snippet: Core-genome MLST (cgMLST) analysis for 286 |