Review



mrna seq library preparation kit  (ABclonal Biotechnology)


Bioz Verified Symbol ABclonal Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    ABclonal Biotechnology mrna seq library preparation kit
    Mrna Seq Library Preparation Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/mRNA-seq+Lib+Prep+Kit/pmc13614436-71-1-6
    Average 98 stars, based on 296 article reviews
    mrna seq library preparation kit - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Construct:

    Article Title: Integrated WGCNA, Network Pharmacology, and UPLC-MS/MS Profiling for Investigating the Antitumor Effects of the Kansui Radix Dichloromethane Fraction Against Renal Cell Carcinoma with Experimental Validation
    Article Snippet: .. Stranded mRNA libraries were constructed using the ABclonal mRNA-seq Lib Prep Kit following the manufacturer’s protocol. .. The libraries were sequenced on an Illumina Novaseq X Plus platform in paired-end mode, generating approximately 6–8 Gb of clean data per sample.

    Article Title: Cistanche deserticola Polysaccharide Ameliorates Cyclophosphamide-Induced Splenic Immunosuppression in Mice: Integrated Transcriptomic and Proteomic Analyses of Immune Modulation
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and tested for purity by a Nanodrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was assessed by an Agilent Bioanalyzer 4150 (Agilent Technologies, Santa Clara, CA, USA). .. QC-qualified samples were used to construct double-ended sequencing libraries using the AB clonal mRNA-seq Lib Prep Kit (ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China): briefly, 1 μg of total RNA was fragmented by oligo(dT)-enriched magnetic beads; double-stranded cDNA was synthesized with random primers, ligated to Illumina junctions and amplified by PCR; and the libraries were QC-qualified and amplified on an Illumina Nova Seq. .. After library quality control, the libraries were sequenced on an Illumina Nova Seq 6000/MGISEQ-T7 platform (Illumina Inc., San Diego, CA, USA/MGI Tech Co., Ltd., Shenzhen, Guangdong, China) at 150 bp (≥6 Gb of clean data per sample).

    Isolation:

    Article Title: Gut microbiota-derived propionate mediates the protective effects of green banana resistant starch on ulcerative colitis by inhibiting HDAC1 to promote HIF-1α acetylation.
    Article Snippet: Ulcerative colitis (UC) is a chronic inflammatory bowel disease with increasing global incidence, lacking specific targeted drugs.. Green banana resistant starch (RS) has been shown to possess antioxidant effects and alleviate gastric ulcers and diarrhea, suggesting its potential therapeutic value for UC.. However, its specific efficacy and mechanism of action remain unclear.

    Reverse Transcription:

    Article Title: Gut microbiota-derived propionate mediates the protective effects of green banana resistant starch on ulcerative colitis by inhibiting HDAC1 to promote HIF-1α acetylation.
    Article Snippet: Ulcerative colitis (UC) is a chronic inflammatory bowel disease with increasing global incidence, lacking specific targeted drugs.. Green banana resistant starch (RS) has been shown to possess antioxidant effects and alleviate gastric ulcers and diarrhea, suggesting its potential therapeutic value for UC.. However, its specific efficacy and mechanism of action remain unclear.

    Sequencing:

    Article Title: Cistanche deserticola Polysaccharide Ameliorates Cyclophosphamide-Induced Splenic Immunosuppression in Mice: Integrated Transcriptomic and Proteomic Analyses of Immune Modulation
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and tested for purity by a Nanodrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was assessed by an Agilent Bioanalyzer 4150 (Agilent Technologies, Santa Clara, CA, USA). .. QC-qualified samples were used to construct double-ended sequencing libraries using the AB clonal mRNA-seq Lib Prep Kit (ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China): briefly, 1 μg of total RNA was fragmented by oligo(dT)-enriched magnetic beads; double-stranded cDNA was synthesized with random primers, ligated to Illumina junctions and amplified by PCR; and the libraries were QC-qualified and amplified on an Illumina Nova Seq. .. After library quality control, the libraries were sequenced on an Illumina Nova Seq 6000/MGISEQ-T7 platform (Illumina Inc., San Diego, CA, USA/MGI Tech Co., Ltd., Shenzhen, Guangdong, China) at 150 bp (≥6 Gb of clean data per sample).

    Article Title: C-1101 multi-protein platelet and plasma-derived therapeutic to treat chronic lumbosacral radiculopathy
    Article Snippet: .. Samples were sequenced at Novogene, Inc. Novogene protocols included poly-dt magnetic bead filtering for mRNA, followed by library prep using ABclonal mRNA-seq Lib Prep Kit, then 150bp paired end sequencing on the Illumina NovaSeq X Plus. .. Chemotherapy (paclitaxel)-induced peripheral neuropathy (PIPN) ( ) was induced in male C57BL6/J mice (sham group 10 mice/group, paclitaxel + vehicle or C-1101 15 mice/group) to assess effect of systemic C-1101 administration on allodynia, and systemic cytokine levels (Charles River Discovery Services, Finland).

    Magnetic Beads:

    Article Title: Cistanche deserticola Polysaccharide Ameliorates Cyclophosphamide-Induced Splenic Immunosuppression in Mice: Integrated Transcriptomic and Proteomic Analyses of Immune Modulation
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and tested for purity by a Nanodrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was assessed by an Agilent Bioanalyzer 4150 (Agilent Technologies, Santa Clara, CA, USA). .. QC-qualified samples were used to construct double-ended sequencing libraries using the AB clonal mRNA-seq Lib Prep Kit (ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China): briefly, 1 μg of total RNA was fragmented by oligo(dT)-enriched magnetic beads; double-stranded cDNA was synthesized with random primers, ligated to Illumina junctions and amplified by PCR; and the libraries were QC-qualified and amplified on an Illumina Nova Seq. .. After library quality control, the libraries were sequenced on an Illumina Nova Seq 6000/MGISEQ-T7 platform (Illumina Inc., San Diego, CA, USA/MGI Tech Co., Ltd., Shenzhen, Guangdong, China) at 150 bp (≥6 Gb of clean data per sample).

    Synthesized:

    Article Title: Cistanche deserticola Polysaccharide Ameliorates Cyclophosphamide-Induced Splenic Immunosuppression in Mice: Integrated Transcriptomic and Proteomic Analyses of Immune Modulation
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and tested for purity by a Nanodrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was assessed by an Agilent Bioanalyzer 4150 (Agilent Technologies, Santa Clara, CA, USA). .. QC-qualified samples were used to construct double-ended sequencing libraries using the AB clonal mRNA-seq Lib Prep Kit (ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China): briefly, 1 μg of total RNA was fragmented by oligo(dT)-enriched magnetic beads; double-stranded cDNA was synthesized with random primers, ligated to Illumina junctions and amplified by PCR; and the libraries were QC-qualified and amplified on an Illumina Nova Seq. .. After library quality control, the libraries were sequenced on an Illumina Nova Seq 6000/MGISEQ-T7 platform (Illumina Inc., San Diego, CA, USA/MGI Tech Co., Ltd., Shenzhen, Guangdong, China) at 150 bp (≥6 Gb of clean data per sample).

    Amplification:

    Article Title: Cistanche deserticola Polysaccharide Ameliorates Cyclophosphamide-Induced Splenic Immunosuppression in Mice: Integrated Transcriptomic and Proteomic Analyses of Immune Modulation
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and tested for purity by a Nanodrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was assessed by an Agilent Bioanalyzer 4150 (Agilent Technologies, Santa Clara, CA, USA). .. QC-qualified samples were used to construct double-ended sequencing libraries using the AB clonal mRNA-seq Lib Prep Kit (ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China): briefly, 1 μg of total RNA was fragmented by oligo(dT)-enriched magnetic beads; double-stranded cDNA was synthesized with random primers, ligated to Illumina junctions and amplified by PCR; and the libraries were QC-qualified and amplified on an Illumina Nova Seq. .. After library quality control, the libraries were sequenced on an Illumina Nova Seq 6000/MGISEQ-T7 platform (Illumina Inc., San Diego, CA, USA/MGI Tech Co., Ltd., Shenzhen, Guangdong, China) at 150 bp (≥6 Gb of clean data per sample).

    Polymerase Chain Reaction:

    Article Title: Cistanche deserticola Polysaccharide Ameliorates Cyclophosphamide-Induced Splenic Immunosuppression in Mice: Integrated Transcriptomic and Proteomic Analyses of Immune Modulation
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and tested for purity by a Nanodrop ND-2000 (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was assessed by an Agilent Bioanalyzer 4150 (Agilent Technologies, Santa Clara, CA, USA). .. QC-qualified samples were used to construct double-ended sequencing libraries using the AB clonal mRNA-seq Lib Prep Kit (ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China): briefly, 1 μg of total RNA was fragmented by oligo(dT)-enriched magnetic beads; double-stranded cDNA was synthesized with random primers, ligated to Illumina junctions and amplified by PCR; and the libraries were QC-qualified and amplified on an Illumina Nova Seq. .. After library quality control, the libraries were sequenced on an Illumina Nova Seq 6000/MGISEQ-T7 platform (Illumina Inc., San Diego, CA, USA/MGI Tech Co., Ltd., Shenzhen, Guangdong, China) at 150 bp (≥6 Gb of clean data per sample).

    Purification:

    Article Title: A Magneto‐Mechanically Activated Nerve Guidance Conduit Promotes Peripheral Nerve Regeneration via TIMP1‐Mediated Membrane Tension Transfer
    Article Snippet: Total RNA was extracted from MSCs with/without FP + MF treatment in the co-culture system using TRIzol Reagent (15596018CN, ThermoFisher) and sent to Sinotech Genomics (Shanghai, China) for sequencing. .. mRNA libraries were prepared using the Illumina mRNA-seq Lib Prep Kit (RK20302, ABclonal), and the purified cDNA libraries were quantified with Qubit 2.0 Fluorometer (Life Technologies) and validated using an Agilent 2100 Bioanalyzer. .. Sequencing was conducted on an Illumina NovaSeq 6000 platform.

    RNA Sequencing:

    Article Title: Ten‐Eleven Translocation Enzymes Control the Rate and Mode of Retinal Progenitor Cell Division in the Developing Retina
    Article Snippet: .. The mRNA‐seq library preparation Kit (RK20302, ABclonal, Woburn, MA, USA) was utilized according to the manufacturer's instructions to prepare RNA‐seq libraries, which were sequenced on the Illumina NovaSeq X Plus using 2 × 150 PE configuration. ..



    Similar Products

    98
    TaKaRa smart seq mrna lp kit
    Smart Seq Mrna Lp Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/SMART-Seq+mRNA+LP/pm42826831-64-6-10
    Average 98 stars, based on 1 article reviews
    smart seq mrna lp kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    96
    Vazyme Biotech Co vahts universal v6 rna seq library prep kit for illumina
    Vahts Universal V6 Rna Seq Library Prep Kit For Illumina, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/VAHTS+Universal+V8+RNA-seq+Library+Prep+Kit+for+Illumina/pm42822321-126-5-14
    Average 96 stars, based on 1 article reviews
    vahts universal v6 rna seq library prep kit for illumina - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    86
    Thermo Fisher ion total rna seq kit v2
    Ion Total Rna Seq Kit V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/nct04488614-6-19-24
    Average 86 stars, based on 1 article reviews
    ion total rna seq kit v2 - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Karius Inc mcfdna seq
    Mcfdna Seq, supplied by Karius Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/mcfdna+seq/pm41780551-70-29-42
    Average 86 stars, based on 1 article reviews
    mcfdna seq - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    Qiagen rna seq analysis
    Rna Seq Analysis, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/miRNeasy+Mini+Kit/pmc13651848-272-1-12
    Average 99 stars, based on 1 article reviews
    rna seq analysis - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    Vazyme Biotech Co vahts universal v8 rna seq prep kit
    Vahts Universal V8 Rna Seq Prep Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/VAHTS+Universal+V8+RNA-seq+Library+Prep+Kit+for+MGI/pm42816891-128-7-13
    Average 96 stars, based on 1 article reviews
    vahts universal v8 rna seq prep kit - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Complete Genomics Inc stereo-cite proteo-transcriptomics set
    Stereo Cite Proteo Transcriptomics Set, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/Stereo-CITE+Proteo-Transcriptomics+Set/custom%40211pt11114%4042748198
    Average 99 stars, based on 1 article reviews
    stereo-cite proteo-transcriptomics set - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext enzymatic methyl seq em seq kit
    Nebnext Enzymatic Methyl Seq Em Seq Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/NEBNext+Enzymatic+Methyl-seq+Kit/pm42809362-157-10-10
    Average 99 stars, based on 1 article reviews
    nebnext enzymatic methyl seq em seq kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results