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slide seq spatial transcriptomics experiment  (TaKaRa)


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    Structured Review

    TaKaRa slide seq spatial transcriptomics experiment
    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and <t>transcriptomics</t> in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)
    Slide Seq Spatial Transcriptomics Experiment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seeker+spatial+transcriptomics+kit/Seeker+Spatial+Transcriptomics+Kit/pmc12867689-204-0-8
    Average 95 stars, based on 153 article reviews
    slide seq spatial transcriptomics experiment - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection"

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2504742123

    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)
    Figure Legend Snippet: AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Techniques Used: In Situ, Clone Assay, Staining, Marker

    Related Articles

    Spatial Transcriptomics:

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection
    Article Snippet: .. Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions. .. In brief, a 10-μm thickness tissue section from each collected fresh-frozen PLN was mounted on a 3-mm x 3-mm spatially indexed bead surface Curio Seeker tile.

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection
    Article Snippet: .. The AIR-SPACE protocol begins with a 10-μm tissue section mounted onto a spatial transcriptomics array (Curio Seeker, Materials and Methods ). ..

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection.
    Article Snippet: .. The AIR-SPACE protocol begins with a 10-μm tissue section mounted onto a spatial transcriptomics array (Curio Seeker, Materials and Methods ). ..

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection.
    Article Snippet: Slide-Seq Spatial Transcriptomics Library Preparation and Sequencing. .. Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions. .. In brief, a 10-μm thickness tissue section from each collected fresh-frozen PLN was mounted on a 3-mm x 3-mm spatially indexed bead surface Curio Seeker tile.

    Expressing:

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..

    Activation Assay:

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..

    Generated:

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..



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    Image Search Results


    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection

    doi: 10.1073/pnas.2504742123

    Figure Lengend Snippet: AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Article Snippet: Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions.

    Techniques: In Situ, Clone Assay, Staining, Marker