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SibEnzyme ltd se-buffer y
Se Buffer Y, supplied by SibEnzyme ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/se-buffer+y/buffer+w/pmc10968005-65-25-27
Average 90 stars, based on 1 article reviews
se-buffer y - by Bioz Stars, 2026-11
90/100 stars

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Article Title: The hTERT and iCasp9 Transgenes Affect EOMES and T-BET Levels in NK Cells and the Introduction of Both Genes Improves NK Cell Proliferation in Response to IL2 and IL15 Stimulation
Article Snippet: Then, the restriction of a target plasmid xlox(GFP)hTERT (Addgene #69809) and PCR fragments was carried out by endonuclease NotI (isoshizomer CciNI, SibEnzyme, Novosibirsk, Russia) in SE-buffer Y (SibEnzyme).

Article Title: Viral metagenomics in wild ducks reveals the presence of seadornaviruses in Siberia.
Article Snippet: members of the genus – Banna virus (BAV, Seadornavirus bannaense) and Liao ning virus (LNV, Seadornavirus liaoningense) – have been found to be associated with vertebrates.. LNV has the ability to infect mammalian cells, and BAV was originally isolated from serum and cerebrospinal fluid of patients with fever and encephalitis in China [2, 3].. Interestingly, a virus related to Banna virus was detected in fish in Hungary, using viral metagenomics [4].

Article Title: Ab initio DNA synthesis by Bst polymerase in the presence of nicking endonucleases Nt.AlwI, Nb.BbvCI, and Nb.BsmI.
Article Snippet: In the case of Nb.BbvCI the SE-buffer G (SibEnzyme) contained 1 mM Tris-HCl, 1 mM MgCl2, 5 mM NaCl and 0.1 mM dithiothreitol (DTT), pH 7.6 at 25 °C.

Article Title: DNA Methylation and Prospects for Predicting the Therapeutic Effect of Neoadjuvant Chemotherapy for Triple-Negative and Luminal B Breast Cancer.
Article Snippet: Enzymatic hydrolysis was carried out in a total volume of 35 μL, containing 10 units of restriction endonuclease, 3.5 μL of 10× SE-buffer Y (SibEnzyme, Russia), and 60 ng of DNA for 12 h at 50 ◦C.

Plasmid Preparation:

Article Title: A Method for Assessing the Efficiency of the Nucleotide Excision Repair System Ex Vivo
Article Snippet: .. A 38-bp segment (622–660 bp, MCS) was excised from the pTagRFP-N plasmid using the restriction endonucleases HindIII and BamHI (SibEnzyme) by incubation of 1 μg of the plasmid with 1 U HindIII and 1 U BamHI in a W buffer (SibEnzyme) at 37°C for 1 h. After enzyme inactivation (70°C, 20 min) and DNA precipitation according to the standard procedure [ ], the linearized plasmid was dissolved in water and a 40-fold molar excess of the DNA insert, 2 U T4 DNA ligase (SibEnzyme) in a SE buffer, and 5 mM ATP were added. ..

Article Title: Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus
Article Snippet: .. Synthesis and methylation of recombinant HBV cccDNA 1.0-mer linear HBV genome was released from the HBV-encoding plasmid pCH-HBV by incubating with PsiI and PstI restriction enzymes in Y buffer (Sibenzyme) overnight. .. The HBV genome (3182 bp) was gel purified by electrophoresis and isolated using the Qiagen gel extraction kit.

Incubation:

Article Title: A Method for Assessing the Efficiency of the Nucleotide Excision Repair System Ex Vivo
Article Snippet: .. A 38-bp segment (622–660 bp, MCS) was excised from the pTagRFP-N plasmid using the restriction endonucleases HindIII and BamHI (SibEnzyme) by incubation of 1 μg of the plasmid with 1 U HindIII and 1 U BamHI in a W buffer (SibEnzyme) at 37°C for 1 h. After enzyme inactivation (70°C, 20 min) and DNA precipitation according to the standard procedure [ ], the linearized plasmid was dissolved in water and a 40-fold molar excess of the DNA insert, 2 U T4 DNA ligase (SibEnzyme) in a SE buffer, and 5 mM ATP were added. ..

Methylation:

Article Title: Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus
Article Snippet: .. Synthesis and methylation of recombinant HBV cccDNA 1.0-mer linear HBV genome was released from the HBV-encoding plasmid pCH-HBV by incubating with PsiI and PstI restriction enzymes in Y buffer (Sibenzyme) overnight. .. The HBV genome (3182 bp) was gel purified by electrophoresis and isolated using the Qiagen gel extraction kit.

Recombinant:

Article Title: Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus
Article Snippet: .. Synthesis and methylation of recombinant HBV cccDNA 1.0-mer linear HBV genome was released from the HBV-encoding plasmid pCH-HBV by incubating with PsiI and PstI restriction enzymes in Y buffer (Sibenzyme) overnight. .. The HBV genome (3182 bp) was gel purified by electrophoresis and isolated using the Qiagen gel extraction kit.



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