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GenScript corporation 1×sds loading buffer
1×Sds Loading Buffer, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds+loading+buffer+(1)/sds+loading+buffer/pm40344394-387-38-41
Average 90 stars, based on 1 article reviews
1×sds loading buffer - by Bioz Stars, 2026-09
90/100 stars

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Transfection:

Article Title: Cnidaria XIAP activates caspase-mediated cell death
Article Snippet: The polyps were stained with PI (2 μg/ml) for 15 min and then observed with a fluorescence microscope (Olympus, Tokyo, Japan). .. HEK293T cells transfected with indicated plasmids were lysed with lysis buffer (Beyotime, Shanghai, China), and the lysate was boiled in SDS loading buffer and electrophoresed in 12% SDS-PAGE (GenScript, Piscataway, NJ). ..

Lysis:

Article Title: Cnidaria XIAP activates caspase-mediated cell death
Article Snippet: The polyps were stained with PI (2 μg/ml) for 15 min and then observed with a fluorescence microscope (Olympus, Tokyo, Japan). .. HEK293T cells transfected with indicated plasmids were lysed with lysis buffer (Beyotime, Shanghai, China), and the lysate was boiled in SDS loading buffer and electrophoresed in 12% SDS-PAGE (GenScript, Piscataway, NJ). ..

Article Title: Novel chemotype NLRP3 inhibitors that target the CRID3-binding pocket with high potency
Article Snippet: LDH activity in the culture medium was assayed using CytoTox 96 Non-Radioactive Cytotoxicity Assay (G1780; Promega). .. Cell lysates and culture supernatants were incubated with cell lysis buffer (20 mM Tris–HCl [pH 7.4], 200 mM NaCl, 1% Nonidet P-40) and were denatured in lithium dodecyl sulfate sample buffer (M00676; GenScript) and boiled at 95°C for 10 min. SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against caspase-1 (AG-20B-0042-C100; Adipogen), IL-1β (GTX74034; Genetex), GSDMD (ab219800; Abcam), and β-actin (SC-47778HRP; Santa Cruz Biotechnology). .. Peroxidase-AffiniPure Goat Anti-Mouse (115-035-146; Jackson Immunoresearch Laboratories) or Anti-Rabbit (111-035-144; Jackson Immunoresearch Laboratories) secondary antibodies were used to detect proteins by enhanced chemiluminescence (34580; Thermo Fisher Scientific).

SDS Page:

Article Title: Cnidaria XIAP activates caspase-mediated cell death
Article Snippet: The polyps were stained with PI (2 μg/ml) for 15 min and then observed with a fluorescence microscope (Olympus, Tokyo, Japan). .. HEK293T cells transfected with indicated plasmids were lysed with lysis buffer (Beyotime, Shanghai, China), and the lysate was boiled in SDS loading buffer and electrophoresed in 12% SDS-PAGE (GenScript, Piscataway, NJ). ..

Incubation:

Article Title: PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma.
Article Snippet: Co-Immunoprecipitation (co-IP) and LC-MS/MS Analysis: The cells were lysed in IP/Western lysis buffer (Beyotime, China) containing a proteinase and phosphatase inhibitor cocktail (Selleck, USA). .. The cell lysates were incubated with antibodies in a rotating incubator overnight at 4°C, followed by coincubation with protein A/G magnetic beads (Bimake, China) at 4°C for 6 h. The beads were washed with PBST and boiled in 1×SDS loading buffer (GenScript, China), and the binding proteins were analyzed via LC‒MS/MS or Western blot analysis. .. Protein samples were then analyzed using a Q Exactive Plus mass spectrometer (Thermo Scientific, Massachusetts, USA) in conjunction with the UltiMate 3000 RSLC nanosystem (Thermo Fisher) for detailed protein identification and quantification.

Article Title: Novel chemotype NLRP3 inhibitors that target the CRID3-binding pocket with high potency
Article Snippet: LDH activity in the culture medium was assayed using CytoTox 96 Non-Radioactive Cytotoxicity Assay (G1780; Promega). .. Cell lysates and culture supernatants were incubated with cell lysis buffer (20 mM Tris–HCl [pH 7.4], 200 mM NaCl, 1% Nonidet P-40) and were denatured in lithium dodecyl sulfate sample buffer (M00676; GenScript) and boiled at 95°C for 10 min. SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against caspase-1 (AG-20B-0042-C100; Adipogen), IL-1β (GTX74034; Genetex), GSDMD (ab219800; Abcam), and β-actin (SC-47778HRP; Santa Cruz Biotechnology). .. Peroxidase-AffiniPure Goat Anti-Mouse (115-035-146; Jackson Immunoresearch Laboratories) or Anti-Rabbit (111-035-144; Jackson Immunoresearch Laboratories) secondary antibodies were used to detect proteins by enhanced chemiluminescence (34580; Thermo Fisher Scientific).

Magnetic Beads:

Article Title: PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma.
Article Snippet: Co-Immunoprecipitation (co-IP) and LC-MS/MS Analysis: The cells were lysed in IP/Western lysis buffer (Beyotime, China) containing a proteinase and phosphatase inhibitor cocktail (Selleck, USA). .. The cell lysates were incubated with antibodies in a rotating incubator overnight at 4°C, followed by coincubation with protein A/G magnetic beads (Bimake, China) at 4°C for 6 h. The beads were washed with PBST and boiled in 1×SDS loading buffer (GenScript, China), and the binding proteins were analyzed via LC‒MS/MS or Western blot analysis. .. Protein samples were then analyzed using a Q Exactive Plus mass spectrometer (Thermo Scientific, Massachusetts, USA) in conjunction with the UltiMate 3000 RSLC nanosystem (Thermo Fisher) for detailed protein identification and quantification.

Binding Assay:

Article Title: PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma.
Article Snippet: Co-Immunoprecipitation (co-IP) and LC-MS/MS Analysis: The cells were lysed in IP/Western lysis buffer (Beyotime, China) containing a proteinase and phosphatase inhibitor cocktail (Selleck, USA). .. The cell lysates were incubated with antibodies in a rotating incubator overnight at 4°C, followed by coincubation with protein A/G magnetic beads (Bimake, China) at 4°C for 6 h. The beads were washed with PBST and boiled in 1×SDS loading buffer (GenScript, China), and the binding proteins were analyzed via LC‒MS/MS or Western blot analysis. .. Protein samples were then analyzed using a Q Exactive Plus mass spectrometer (Thermo Scientific, Massachusetts, USA) in conjunction with the UltiMate 3000 RSLC nanosystem (Thermo Fisher) for detailed protein identification and quantification.

Western Blot:

Article Title: PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma.
Article Snippet: Co-Immunoprecipitation (co-IP) and LC-MS/MS Analysis: The cells were lysed in IP/Western lysis buffer (Beyotime, China) containing a proteinase and phosphatase inhibitor cocktail (Selleck, USA). .. The cell lysates were incubated with antibodies in a rotating incubator overnight at 4°C, followed by coincubation with protein A/G magnetic beads (Bimake, China) at 4°C for 6 h. The beads were washed with PBST and boiled in 1×SDS loading buffer (GenScript, China), and the binding proteins were analyzed via LC‒MS/MS or Western blot analysis. .. Protein samples were then analyzed using a Q Exactive Plus mass spectrometer (Thermo Scientific, Massachusetts, USA) in conjunction with the UltiMate 3000 RSLC nanosystem (Thermo Fisher) for detailed protein identification and quantification.

other:

Article Title: Alcogels based on secalin and konjac glucomannan composites for the controlled release of curcumin.
Article Snippet: Alcogel has been increasingly applied in foods, cosmetics, and pharmaceutical industries.. However, their application is limited by the lack of efficient biomacromolecule-based gelators.. Herein, we present our discovery of secalin, a prolamin from rye, combined with konjac glucomannan (KGM) as novel food-grade gelators.

Article Title: The HIV-1 capsid serves as a nanoscale reaction vessel for reverse transcription
Article Snippet: Pelleted virions were dissolved in SDS-PAGE sample buffer and subjected to electrophoresis on precast 4–20% gradient gels using Tris-MOPS running buffer (Genscript).

Electrophoresis:

Article Title: 25-Hydroxycholesterol inhibits Hantavirus infection by reprogramming cholesterol metabolism.
Article Snippet: Hantavirus causes two types of acute diseases: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome.. It is a major health concern due to its high mortality and lack of effective treatment.. Type I interferon treatment has been suggested to be effective against hantavirus when treated in advance.

Nucleic Acid Electrophoresis:

Article Title: 25-Hydroxycholesterol inhibits Hantavirus infection by reprogramming cholesterol metabolism.
Article Snippet: Hantavirus causes two types of acute diseases: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome.. It is a major health concern due to its high mortality and lack of effective treatment.. Type I interferon treatment has been suggested to be effective against hantavirus when treated in advance.



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