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1 ×sds loading buffer (Beyotime)

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Beyotime 1 ×sds loading buffer
1 ×Sds Loading Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds+loading+buffer+(1)/sds+loading+buffer/pm40156971-147-4-12
Average 90 stars, based on 1 article reviews

1 ×sds loading buffer - by Bioz Stars, 2026-09

90/100 stars

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Article Title: UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice
Article Snippet: The supernatant was transferred to a new tube and 5 × SDS-PAGE protein loading buffer (Beyotime Biotechnology, P0015L) was added.

Article Title: PHB1 and PHB2 positively regulate IFN-β production by targeting MAVS in ducks
Article Snippet: After centrifugation (12,000 rpm, 4 °C, 5 min), the supernatant was collected and mixed with 5 × SDS-PAGE Loading Buffer (Beyotime, Shanghai, China), then denatured at 100 °C for 7 min. Proteins were separated by 10 % SDS-PAGE and transferred to PVDF membranes.

Article Title: Exploration of common pathogenic genes between cerebral amyloid angiopathy and insomnia based on bioinformatics and experimental validation
Article Snippet: Proteins were diluted in 5× SDS sample buffer (Beyotime, China) and boiled at 100 °C for 10 min.

Article Title: Blood–brain barrier disruption and neuroinflammation in the hippocampus of a cardiac arrest porcine model: Single-cell RNA sequencing analysis
Article Snippet: Protein samples extracted from hippocampal tissue were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Beyotime Biotechnology, Shanghai, China, Cat# P0015) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Burlington, MA, USA, Cat# ISEQ00010).

Centrifugation:

Article Title: Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway
Article Snippet: After transfection, cells were harvested by centrifugation, washed with ice-cold 1 × PBS, and lysed using radioimmunoprecipitation assay (RIPA) buffer (Beyotime). .. The lysed proteins in the supernatant were collected following centrifugation and mixed with 5 × SDS loading buffer (Beyotime). ..

Article Title: Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala: association with enhanced cued fear expression
Article Snippet: .. Supernatants of samples were collected after 15 minutes (12,000 × g ) centrifugation of homogenates at 4°C, and then mixed with loading buffer (Beyotime, Cat# P0015B). ..

SDS Page:

Article Title: Transcriptional factors AcNAC1/2 synergistically orchestrate kiwifruit chlorophyll degradation
Article Snippet: Commercially grown kiwifruit usually show a high degree of flesh color, generally delineated as greenand yellow-fleshed cultivars (include the red-cored cultivars).. Chlorophyll degradation during kiwifruit development is a crucial process for the appearance of more ‘ripe’ red or yellow/orange color.. Previously, transcription factor AcNAC2 was characterized as a master regulator on kiwifruit chlorophyll degradation, yet the underlying regulatory network of AcNAC2 needs to be further elucidated.

Article Title: DDX3X/MAVS alleviates doxorubicin-induced cardiotoxicity by regulating stress granules
Article Snippet: Protein concentration was detected using a BCA Protein Assay Kit (cat. no. P0012S; Beyotime Institute of Biotechnology). .. Protein loading buffer (cat. no. P0015; Beyotime Institute of Biotechnology) was then added, mixed and boiled for 10 min. Total protein (20–50 μg) was subsequently separated by SDS-PAGE on 12.5% gels (cat. no. PG113; Epizyme; Ipsen Pharma) and transferred to 0.45-μm PVDF membranes (cat. no. IPVH00010; MilliporeSigma). .. The PVDF membranes were blocked with 5% skim milk powder at room temperature for 2 h, incubated with specific primary antibodies at 4°C overnight, washed three times with TBS containing 0.1% Tween-20 and incubated with HRP-conjugated secondary antibodies at room temperature for 1 h. The blots were visualized using ECL Enhanced Plus Kit (cat. no. RM00021P; ABclonal Biotech Co., Ltd.), images were acquired using a gel imager (ChemiDoc; Bio-Rad Laboratories, Inc.) and image grayscale values were analyzed using ImageJ v1.8.0.112 software (National Institutes of Health).