sanger sequencing data (Synthego Inc)
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Synthego Inc
sanger sequencing data
Sanger Sequencing Data, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+sequence+data/sanger+sequencing/pmc12727531-338-2-8
Average 86 stars, based on 1 article reviews
Sanger Sequencing Data, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+sequence+data/sanger+sequencing/pmc12727531-338-2-8
Average 86 stars, based on 1 article reviews
sanger sequencing data - by Bioz Stars,
2026-11
86/100 stars
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Isolation:Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication. Article Snippet: .. Recql4Δ/Δ sgKlhdc3 K1B12 and Recql4Δ/Δ sgKlhdc3 K1E1 are independently targeted and isolated clones. (D) The knockout efficiency of Mcm10 was measured through Knock-Out:Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication. Article Snippet: .. Recql4Δ/Δ sgKlhdc3 K1B12 and Recql4Δ/Δ sgKlhdc3 K1E1 are independently targeted and isolated clones. (D) The knockout efficiency of Mcm10 was measured through Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via Sequencing:Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication. Article Snippet: .. Recql4Δ/Δ sgKlhdc3 K1B12 and Recql4Δ/Δ sgKlhdc3 K1E1 are independently targeted and isolated clones. (D) The knockout efficiency of Mcm10 was measured through Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via Article Title: DRP1 depletion protects NK cells from hypoxia-induced dysfunction. Article Snippet: .. Scale bar = 5 μm; D. Schematic of the DRP1 gene with target sites for the three guide RNAs used for CRISPR editing; Article Title: Autoinhibitory control of MLKL governs pseudokinase domain phosphorylation and oligomerization during necroptosis. Article Snippet: HEK293T cells (derived from a female human fetus) were purchased from ATCC and cultured in DMEM, supplemented with 10% Cosmic Calf Serum and 1% Anti-Anti. .. Knockouts were validated by Western blot and Article Title: Novel mouse reporter models for the detection of genome editing events in vivo Article Snippet: .. Quantification of editing activity was performed by analyzing the Article Title: MYOM1 deficiency leads to dilated cardiomyopathy by disrupting the homeostasis of sarcoplasmic reticulum Article Snippet: .. The results of Article Title: MYOM1 deficiency leads to dilated cardiomyopathy by disrupting the homeostasis of sarcoplasmic reticulum. Article Snippet: .. The results of Clone Assay:Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via Nucleic Acid Electrophoresis:Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via Polymerase Chain Reaction:Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via Biomarker Discovery:Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via Next-Generation Sequencing:Article Title: Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells. Article Snippet: A Lonza 4D Amaxa nucleofector was used with SF solution and program CM-156. .. Clones were seeded at an approximate density of 1 cell per 100 μL per well in a 96-well plate and screened first by gel electrophoresis of PCR-amplified genomic region bound by primers rT5A-CrisGeno-F and rT5A-CrisGeno-R. Knockout and wildtype clones were then assessed via CRISPR:Article Title: DRP1 depletion protects NK cells from hypoxia-induced dysfunction. Article Snippet: .. Scale bar = 5 μm; D. Schematic of the DRP1 gene with target sites for the three guide RNAs used for CRISPR editing; Expressing:Article Title: DRP1 depletion protects NK cells from hypoxia-induced dysfunction. Article Snippet: .. Scale bar = 5 μm; D. Schematic of the DRP1 gene with target sites for the three guide RNAs used for CRISPR editing; Western Blot:Article Title: Autoinhibitory control of MLKL governs pseudokinase domain phosphorylation and oligomerization during necroptosis. Article Snippet: HEK293T cells (derived from a female human fetus) were purchased from ATCC and cultured in DMEM, supplemented with 10% Cosmic Calf Serum and 1% Anti-Anti. .. Knockouts were validated by Western blot and Activity Assay:Article Title: Novel mouse reporter models for the detection of genome editing events in vivo Article Snippet: .. Quantification of editing activity was performed by analyzing the other:Article Title: Pharmacology of nivegacetor (RG6289), a potent and selective gamma secretase modulator in clinical development for the treatment of Alzheimer’s disease Article Snippet: The disruption of both PSEN1 and PSEN2 genes was confirmed by |
![Phylogenetic analysis of measles genotype B3 strains detected in Canada, 2018–2020. (A,B) Maximum likelihood phylogenetic trees of the N450, 450 nucleotides, [(A) 27 sequences] and MF-NCR, 1018 nucleotides, [(B) 21 sequences] loci with 1,000 bootstrap replicates. Bold face indicates the genotype B3 reference sequence [N450 only (A)]. Italics are international sequences obtained from GenBank and for the N450 tree (A) represent WHO-named strains. Thick branch edges correspond to bootstrap values ≥0.7. (D) Maximum clade credibility phylogenetic tree of WGS-t sequences ( n = 21) inferred with BEAST2. The time scale, in years, is at the top. Thick branch edges correspond to posterior values ≥0.7. Brackets with letters correspond to outbreaks and/or phylogenetic clusters analyzed in depth. Meta-data is provided in four columns to the right of the trees. A star symbol in the first column indicates a sequence from a case without a source identified. A check mark in the second column indicates that the case was considered imported. The two color strips indicate association with an outbreak and the MeaNS distinct sequence ID (DSId) assigned to the N450 sequence (including named strain, where applicable) with the legend provided in panel (C). The bracket present in all trees indicates the clade including the MVi/Marikina City.PHL/10.18 N450 named strain. (D) Shades of grey are added to the outbreak strip to denote the identification of related cases as a result of the sequence analysis. High resolution, interactive versions are available at: https://itol.embl.de/shared/GzTsavRiNVS9 .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1582/pmc11611582/pmc11611582__fmicb-15-1475144-g003.jpg)