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sanger sequencing data  (Illumina Inc)


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    Structured Review

    Illumina Inc sanger sequencing data
    Sanger Sequencing Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sanger+sequence+data/sanger+sequencing+data/pm40111248-164-13-13
    Average 90 stars, based on 1 article reviews
    sanger sequencing data - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Applying a modified metabarcoding approach for the sequencing of macrofungal specimens from fungarium collections
    Article Snippet: .. To test whether the Sanger sequencing data matched the Illumina data from the same sequences, BLAST alignments were created and the percent identity (percentage of matching nucleotides in the aligned region) and percent coverage were compared between sequencing methods. .. The results demonstrate a high correlation of percent identity between the Sanger and Illumina sequences in the BLAST alignments (Figure ).

    Article Title: Domestication and evolutionary histories of specialized gut symbionts across cephalotine ants.
    Article Snippet: .. The validity of these findings awaits further testing given the absence of a rarer, Illumina- predicted genotypic variant from the Sanger sequence data for Cephaloticoccus. ..

    Article Title: Population subdivision promoted by a sea-level-change-driven bottleneck: A glimpse from the evolutionary history of the mangrove plant Aegiceras corniculatum.
    Article Snippet: Many coastal plants disperse propagules by seawater.. Therefore, geographical barriers such as landmasses, open oceans (Thornhill et al., 2008), and ocean currents (Guo et al., 2016; Pil et al., 2011; Wee et al., 2014), constrain the margins of their distribution range.. These barriers also cause abrupt discontinuities within the ranges of coastal plants (Hartl & Clark, 1997).

    Article Title: A single-cell genomics pipeline for environmental microbial eukaryotes
    Article Snippet: .. All sequences were verified against the assembled genome, and ambiguous (N) Sanger sequencing reads were corrected with Illumina reads, if necessary. ..

    Article Title: Intersegment Recombination During Influenza A Virus Replication Gives Rise to a Novel Class of Defective Viral Genomes
    Article Snippet: .. Raw Sanger sequencing files are provided in ; Illumina sequencing data are not publicly available due to restrictions imposed by the study funder, OVO Biomanufacturing, but may be made available by the corresponding author upon reasonable request and with permission from OVO Biomanufacturing. ..

    other:

    Article Title: The utility of integrating nanopore sequencing into routine HIV-1 drug resistance surveillance.
    Article Snippet: Several web- based data analysis pipelines are freely available for analysing Sanger and Illumina sequencing data, including RECall, HyDRA, PASeq and MiCall [50, 54, 99, 124].

    Article Title: Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass ( Paspalum notatum Flüggé) using CRISPR/Cas9
    Article Snippet: Using a combination of Sanger and Illumina sequencing data, a total of 8 sgRNA variants were identified in WT bahiagrass which harbored SNPs in the crRNA target or PAM sequences associated with sgRNA1 and sgRNA2 ( ).

    Article Title: Capturing prokaryotic dark matter genomes.
    Article Snippet: 23 Prokaryotes are the most diverse and abundant cellular life forms on Earth.. Most of 24 them, identified by indirect molecular approaches, belong to microbial dark matter.. The 25 advent of metagenomic and single-cell genomic approaches has highlighted the metabolic 26 capabilities of numerous members of this dark matter through genome reconstruction.

    Variant Assay:

    Article Title: Domestication and evolutionary histories of specialized gut symbionts across cephalotine ants.
    Article Snippet: .. The validity of these findings awaits further testing given the absence of a rarer, Illumina- predicted genotypic variant from the Sanger sequence data for Cephaloticoccus. ..



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    DNASTAR sanger sequencing data
    Characteristics of specimens and success of whole genome <t>sequencing.</t> (A–D) Filled bars indicate whole genome sequences achieved, while open or diagonal bars indicate whole genome sequencing failure. Purple color: respiratory specimens; green color: urine. (C) The asterisks indicate a significant difference between mean crossing point (CP) values ( t -test) with a p value <0.0001. (E) Median coverage depth aggregated across 59 measles genomes (WGS-t) generated solely by Illumina sequencing. Depth coverage at each position was obtained through Galaxy using the tool SamTools depth for 59 genomes (samples with termini filled in and sanger patches were excluded from this). Sample termini were trimmed and proportions at each position were determined. Median (solid line) and 25th to 75th percentile (shaded area) values at each nucleotide position were plotted using ggPlot2 in R.
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    Characteristics of specimens and success of whole genome <t>sequencing.</t> (A–D) Filled bars indicate whole genome sequences achieved, while open or diagonal bars indicate whole genome sequencing failure. Purple color: respiratory specimens; green color: urine. (C) The asterisks indicate a significant difference between mean crossing point (CP) values ( t -test) with a p value <0.0001. (E) Median coverage depth aggregated across 59 measles genomes (WGS-t) generated solely by Illumina sequencing. Depth coverage at each position was obtained through Galaxy using the tool SamTools depth for 59 genomes (samples with termini filled in and sanger patches were excluded from this). Sample termini were trimmed and proportions at each position were determined. Median (solid line) and 25th to 75th percentile (shaded area) values at each nucleotide position were plotted using ggPlot2 in R.
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    Image Search Results


    Characteristics of specimens and success of whole genome sequencing. (A–D) Filled bars indicate whole genome sequences achieved, while open or diagonal bars indicate whole genome sequencing failure. Purple color: respiratory specimens; green color: urine. (C) The asterisks indicate a significant difference between mean crossing point (CP) values ( t -test) with a p value <0.0001. (E) Median coverage depth aggregated across 59 measles genomes (WGS-t) generated solely by Illumina sequencing. Depth coverage at each position was obtained through Galaxy using the tool SamTools depth for 59 genomes (samples with termini filled in and sanger patches were excluded from this). Sample termini were trimmed and proportions at each position were determined. Median (solid line) and 25th to 75th percentile (shaded area) values at each nucleotide position were plotted using ggPlot2 in R.

    Journal: Frontiers in Microbiology

    Article Title: Genomic tools for post-elimination measles molecular epidemiology using Canadian surveillance data from 2018–2020

    doi: 10.3389/fmicb.2024.1475144

    Figure Lengend Snippet: Characteristics of specimens and success of whole genome sequencing. (A–D) Filled bars indicate whole genome sequences achieved, while open or diagonal bars indicate whole genome sequencing failure. Purple color: respiratory specimens; green color: urine. (C) The asterisks indicate a significant difference between mean crossing point (CP) values ( t -test) with a p value <0.0001. (E) Median coverage depth aggregated across 59 measles genomes (WGS-t) generated solely by Illumina sequencing. Depth coverage at each position was obtained through Galaxy using the tool SamTools depth for 59 genomes (samples with termini filled in and sanger patches were excluded from this). Sample termini were trimmed and proportions at each position were determined. Median (solid line) and 25th to 75th percentile (shaded area) values at each nucleotide position were plotted using ggPlot2 in R.

    Article Snippet: Consensus sequences of Sanger sequencing data were generated with SeqManPro version 11 software (DNASTAR Lasergene, WI).

    Techniques: Sequencing, Generated, Illumina Sequencing

    Epidemiological curve of confirmed measles cases, 2018–2020, by genotype and sequencing coverage. Top panel: all confirmed cases ( n = 143). Middle panel: cases occurring in 2018 ( n = 29). Bottom panel: cases occurring in 2019 ( n = 113). All panels: blue-fill: genotype D8; yellow-fill: genotype B3; no fill: case without sequences. Bottom two panels: solid-fill: N-450, MF-NCR and WGS-t sequences obtained; diagonal pattern: N-450 and MF-NCR sequences obtained; horizontal pattern: N-450 sequence only. Letter indicates outbreak identifier; lack of a letter indicates isolated case. Check mark (√): case was imported. Asterisk (*): case with unknown source.

    Journal: Frontiers in Microbiology

    Article Title: Genomic tools for post-elimination measles molecular epidemiology using Canadian surveillance data from 2018–2020

    doi: 10.3389/fmicb.2024.1475144

    Figure Lengend Snippet: Epidemiological curve of confirmed measles cases, 2018–2020, by genotype and sequencing coverage. Top panel: all confirmed cases ( n = 143). Middle panel: cases occurring in 2018 ( n = 29). Bottom panel: cases occurring in 2019 ( n = 113). All panels: blue-fill: genotype D8; yellow-fill: genotype B3; no fill: case without sequences. Bottom two panels: solid-fill: N-450, MF-NCR and WGS-t sequences obtained; diagonal pattern: N-450 and MF-NCR sequences obtained; horizontal pattern: N-450 sequence only. Letter indicates outbreak identifier; lack of a letter indicates isolated case. Check mark (√): case was imported. Asterisk (*): case with unknown source.

    Article Snippet: Consensus sequences of Sanger sequencing data were generated with SeqManPro version 11 software (DNASTAR Lasergene, WI).

    Techniques: Sequencing, Isolation

    Phylogenetic analysis of measles genotype B3 strains detected in Canada, 2018–2020. (A,B) Maximum likelihood phylogenetic trees of the N450, 450 nucleotides, [(A) 27 sequences] and MF-NCR, 1018 nucleotides, [(B) 21 sequences] loci with 1,000 bootstrap replicates. Bold face indicates the genotype B3 reference sequence [N450 only (A)]. Italics are international sequences obtained from GenBank and for the N450 tree (A) represent WHO-named strains. Thick branch edges correspond to bootstrap values ≥0.7. (D) Maximum clade credibility phylogenetic tree of WGS-t sequences ( n = 21) inferred with BEAST2. The time scale, in years, is at the top. Thick branch edges correspond to posterior values ≥0.7. Brackets with letters correspond to outbreaks and/or phylogenetic clusters analyzed in depth. Meta-data is provided in four columns to the right of the trees. A star symbol in the first column indicates a sequence from a case without a source identified. A check mark in the second column indicates that the case was considered imported. The two color strips indicate association with an outbreak and the MeaNS distinct sequence ID (DSId) assigned to the N450 sequence (including named strain, where applicable) with the legend provided in panel (C). The bracket present in all trees indicates the clade including the MVi/Marikina City.PHL/10.18 N450 named strain. (D) Shades of grey are added to the outbreak strip to denote the identification of related cases as a result of the sequence analysis. High resolution, interactive versions are available at: https://itol.embl.de/shared/GzTsavRiNVS9 .

    Journal: Frontiers in Microbiology

    Article Title: Genomic tools for post-elimination measles molecular epidemiology using Canadian surveillance data from 2018–2020

    doi: 10.3389/fmicb.2024.1475144

    Figure Lengend Snippet: Phylogenetic analysis of measles genotype B3 strains detected in Canada, 2018–2020. (A,B) Maximum likelihood phylogenetic trees of the N450, 450 nucleotides, [(A) 27 sequences] and MF-NCR, 1018 nucleotides, [(B) 21 sequences] loci with 1,000 bootstrap replicates. Bold face indicates the genotype B3 reference sequence [N450 only (A)]. Italics are international sequences obtained from GenBank and for the N450 tree (A) represent WHO-named strains. Thick branch edges correspond to bootstrap values ≥0.7. (D) Maximum clade credibility phylogenetic tree of WGS-t sequences ( n = 21) inferred with BEAST2. The time scale, in years, is at the top. Thick branch edges correspond to posterior values ≥0.7. Brackets with letters correspond to outbreaks and/or phylogenetic clusters analyzed in depth. Meta-data is provided in four columns to the right of the trees. A star symbol in the first column indicates a sequence from a case without a source identified. A check mark in the second column indicates that the case was considered imported. The two color strips indicate association with an outbreak and the MeaNS distinct sequence ID (DSId) assigned to the N450 sequence (including named strain, where applicable) with the legend provided in panel (C). The bracket present in all trees indicates the clade including the MVi/Marikina City.PHL/10.18 N450 named strain. (D) Shades of grey are added to the outbreak strip to denote the identification of related cases as a result of the sequence analysis. High resolution, interactive versions are available at: https://itol.embl.de/shared/GzTsavRiNVS9 .

    Article Snippet: Consensus sequences of Sanger sequencing data were generated with SeqManPro version 11 software (DNASTAR Lasergene, WI).

    Techniques: Sequencing, Stripping Membranes