Review




Structured Review

DSMZ rt112
Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
Rt112, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/RT-112/pmc13046741-53-0-1
Average 95 stars, based on 157 article reviews
rt112 - by Bioz Stars, 2026-11
95/100 stars

Images

1) Product Images from "Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells"

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

Journal: Scientific Reports

doi: 10.1038/s41598-026-46092-x

Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
Figure Legend Snippet: Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Techniques Used: Fluorescence

Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
Figure Legend Snippet: Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Techniques Used: Irradiation, Control

Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
Figure Legend Snippet: Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Techniques Used: Migration, Positive Control, Control

Related Articles

Cell Culture:

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells.
Article Snippet: .. Cell culture, general cell handling procedures, and sphere formation RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder. .. They have been described to form tumours in nude mice and were verified via authentication testing (Eurofins Genomics; Luxembourg, Luxembourg).

Article Title: Preferential HER4 stimulation preserves neuregulin-induced improvement of myocardial function
Article Snippet: All cancer cell lines were obtained from ATCC, except the human bladder tumor cell line RT-112 which was provided by DSMZ. .. Human NCI-N87 gastric carcinoma (CRL-5822, ATCC, Manassas, VA), T-47D breast carcinoma (HTB-133, ATCC), and RT-112 urinary bladder transitional cell carcinoma (ACC 418, Leibniz Institute DSMZ, Braunschweig, DE) were cultured under standard culture conditions. ..

Article Title: Multi‐omic profiling defines three distinct molecular subtypes of urothelial carcinoma with implications for precision therapy
Article Snippet: UMUC3 cells (Sigma‐Aldrich) were cultured in DMEM‐low glucose medium (PAN‐Biotech). .. RT112, obtained from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ), and 5637 cells (kindly provided by Prof. Holm, Department of Oral and Maxillofacial Surgery, Medical University Innsbruck) were cultured in RPMI 1640 medium (PAN‐Biotech). .. 639V (DSMZ) and VMCUB1 (DSMZ) cells were cultured in DMEM‐high glucose medium (BioWest).



Similar Products

99
CancerTools Org rt112/84
Rt112/84, supplied by CancerTools Org, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/RT112%2F84/custom%40153250%4010%2E64898%2F2026%2E10%2E05%2E756638
Average 99 stars, based on 1 article reviews
rt112/84 - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
CancerTools Org rt112 84
Rt112 84, supplied by CancerTools Org, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/RT112%2F84/pm42529799-94-15-16
Average 99 stars, based on 1 article reviews
rt112 84 - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

rt112  (ATCC)
97
ATCC rt112
Rt112, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/SV-HUC-1/pm42045965-85-1-8
Average 97 stars, based on 1 article reviews
rt112 - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier

99
ATCC rt112 cell line
Rt112 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/RPMI-1640+Medium/us12606541-6898-0-8
Average 99 stars, based on 1 article reviews
rt112 cell line - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

95
DSMZ sphere formation rt112
Sphere Formation Rt112, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/RT-112/pm41922474-70-7-10
Average 95 stars, based on 1 article reviews
sphere formation rt112 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

rt112  (DSMZ)
95
DSMZ rt112
Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
Rt112, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/RT-112/pmc13046741-53-0-1
Average 95 stars, based on 1 article reviews
rt112 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

86
Genechem rt112 cell line
Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
Rt112 Cell Line, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt112/cell+ductal+epithelial+human+line+pancreatic/pm41663867-65-22-28
Average 86 stars, based on 1 article reviews
rt112 cell line - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

Image Search Results


Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: Scientific Reports

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

doi: 10.1038/s41598-026-46092-x

Figure Lengend Snippet: Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

Techniques: Fluorescence

Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: Scientific Reports

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

doi: 10.1038/s41598-026-46092-x

Figure Lengend Snippet: Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

Techniques: Irradiation, Control

Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: Scientific Reports

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

doi: 10.1038/s41598-026-46092-x

Figure Lengend Snippet: Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

Techniques: Migration, Positive Control, Control