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gdna eraser  (TaKaRa)


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    Structured Review

    TaKaRa gdna eraser
    Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/pmc13091134-83-17-20
    Average 99 stars, based on 462 article reviews
    gdna eraser - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: CRISPR/Cas9-mediated knockout reveals non-redundant roles of dsRNases in RNAi efficiency in the melon fly Zeugodacus cucurbitae
    Article Snippet: RNA interference (RNAi) is a powerful tool for functional genomic research in insects and a promising avenue for developing eco-friendly biopesticides; however, its low RNAi efficiency remains a major bottleneck to widespread application.. Double-stranded RNA nucleases (dsRNases) are key regulators of insect RNAi efficiency.. In this study, we identified seven ZcdsRNase genes in the melon fly, Zeugodacus cucurbitae, a globally destructive tephritid pest.

    Article Title: Polyomavirus Drives Cerebellar Lesions in AIDS Monkeys With Ataxia
    Article Snippet: Genomic DNA was purified from tissues, SH‐SY5Y cells, and culture supernatants using a TIANamp Genomic DNA Kit (DP304, TIANGEN, China). .. RNA was reverse transcribed into cDNA using a PrimeScript FAST RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Nucleic acid concentrations were quantified with a NanoDrop ND2000 spectrophotometer (Thermo, USA).

    Article Title: Novel phorbol-12-O-octoate derivatives exert a dual effect by inhibiting de novo HIV-1 infection and reactivating latent virus.
    Article Snippet: .. EGR1 mRNA expression levels were measured after stimulating ACH2 cells with 1 μM HPB-5073 for 1 h and 24 h. Total RNA was extracted from the treated cells using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed using the PrimeScript Fast RT reagent Kit with gDNA Eraser (TaKaRa, Kusatsu, Japan). .. Relative gene expression levels were quantified by RT-qPCR using TB Green Premix Ex Taq or Premix Ex Taq (Probe qPCR) (TaKaRa).

    Article Title: An m 7 G tRNA-derived fragment mediated thermogenic activation of brown adipose tissue through post-transcriptional repression of Uqcr10.
    Article Snippet: Small RNAs were reverse-transcribed 595 using stem-loop RT primers according to the Mir-X miRNA First-Strand Synthesis Kit 596 protocol (Takara Bio, Beijing, China). .. mRNAs were reverse-transcribed with the PrimeScript 597 FAST RT reagent Kit including gDNA Eraser (Takara Bio) following the manufacturer’s 598 instructions. .. qPCR was performed with SYBR Green Master Mix (Takara Bio) on a real-time 599 instrument.

    Expressing:

    Article Title: Novel phorbol-12-O-octoate derivatives exert a dual effect by inhibiting de novo HIV-1 infection and reactivating latent virus.
    Article Snippet: .. EGR1 mRNA expression levels were measured after stimulating ACH2 cells with 1 μM HPB-5073 for 1 h and 24 h. Total RNA was extracted from the treated cells using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed using the PrimeScript Fast RT reagent Kit with gDNA Eraser (TaKaRa, Kusatsu, Japan). .. Relative gene expression levels were quantified by RT-qPCR using TB Green Premix Ex Taq or Premix Ex Taq (Probe qPCR) (TaKaRa).

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    Synthesized:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    Marker:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).

    ALP Assay:

    Article Title: The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.
    Article Snippet: cDNA for miR-339-3p was synthesized via the PrimeScript RT Reagent Kit (Takara, Kyoto, Japan) with gene-specific stem-loop reverse transcription primers. .. Concurrently, cDNA was synthesized using the PrimeScript FAST RT Reagent Kit with gDNA Eraser (Takara) to assess the expression levels of IGF2 and osteogenic differentiation marker genes, including runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP). .. The experiment was conducted in the ABI 7900 system (Applied Biosystems, Foster City, CA) employing the SYBR Green I Master Mix kit (Invitrogen, Waltham, MA).



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