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rna reverse transcription  (TaKaRa)


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    Structured Review

    TaKaRa rna reverse transcription
    Rna Reverse Transcription, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+transcripts/PrimeScript+RT+Master+Mix/pm42100872-272-0-11
    Average 99 stars, based on 26473 article reviews
    rna reverse transcription - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: Monocyte-derived total RNA was extracted using the TransZol Up Plus RNA kit (TransGen Biotech) following manufacturer-recommended protocols. .. First-strand cDNA synthesis was performed with the PrimeScript RT Master Mix (RR037Q; Takara Bio, Shiga, Japan) through reverse transcription of 1 μg total RNA. .. Quantitative PCR amplification was conducted in triplicate reactions (10 μl total volume containing 1 μl cDNA template) using TB Green Premix Ex Taq II (#639676; Takara Bio) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Singapore, Singapore).

    Reverse Transcription:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: Monocyte-derived total RNA was extracted using the TransZol Up Plus RNA kit (TransGen Biotech) following manufacturer-recommended protocols. .. First-strand cDNA synthesis was performed with the PrimeScript RT Master Mix (RR037Q; Takara Bio, Shiga, Japan) through reverse transcription of 1 μg total RNA. .. Quantitative PCR amplification was conducted in triplicate reactions (10 μl total volume containing 1 μl cDNA template) using TB Green Premix Ex Taq II (#639676; Takara Bio) on a 7500 Fast Real-Time PCR System (Applied Biosystems, Singapore, Singapore).

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: Total RNA was extracted from cultured cells and peri-wound tissue samples using the RNeasy Mini Kit (Qiagen, Cat# 74014), following the manufacturer's protocol. .. Reverse transcription was performed using the PrimeScriptTM RT Master Mix (Takara, Cat# RR036A). .. Quantitative real-time PCR (qPCR) was conducted using SYBR Green chemistry on an ABI 7500 Real-Time PCR System (Applied Biosystems).

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Synthesized:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Real-time Polymerase Chain Reaction:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: Total RNA was isolated from SG tissue using TRIzolTM LS Reagent (Invitrogen, Cat. No. 10296010) according to the manufacturer's protocol. .. Complementary DNA (cDNA) was synthesized from 500 to 1000 ng of total RNA using PrimeScript RT Master Mix (TaKaRa, Cat. No. RR036A), and quantitative PCR was performed with TB Green® Premix Ex TaqTM (TaKaRa, Cat. No. RR420A) on a real-time PCR system using gene-specific primers for VEGF, HGF, CD31, and vWF, with GAPDH as the internal reference gene. ..

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    Article Title: Intelligent-responsive hydrogel synergistically mediates immune remodel-antibacterial-angiogenesis cascade for diabetic foot ulcer repair
    Article Snippet: .. Methyl alcohol, Dimethyl sulfoxide (DMSO; General-Reagent, Titan, Shanghai, China); Deferoxamine mesylate (DFO; MCE, New Jersey, USA); Zn(NO 3 ) 2 ·6H 2 O, Mn(NO 3 ) 2 ·4H 2 O, 2-methylimidazole (Aladdin, Shanghai, China); UCMSC-exo (Langfang Kangbao Huitai Biotechnology, Langfang, China); Chitosan (CS; Deacetylation degree ≥95%, Macklin, Shanghai, China), 4-carboxyphenylboronic acid; polyvinyl alcohol (4-CPBA, PVA; Aladdin, Shanghai, China); N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC; Aladdin, Shanghai, China); N-Hydroxysuccinimide (NHS; Aladdin, Shanghai, China); PrimeScript RT Master Mix (Takara, Beijing, China); qPCR SYBR Green Master Mix (Yeasen, Shanghai, China); CD9 antibody (Selleck, USA); TSG101 antibody (Abmart, Shanghai, China); CD86 flow antibody and CD206 flow antibody (BioLegend, USA); IL-6, CD206, HIF-1α, CK14, Ki67, CD31, VEGF and α-SMA (AiFang biological, China). ..

    other:

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: Reverse transcription of RNA was performed using PrimeScriptTM RT Master Mix kit for cDNA synthesis (TaKaRa, RR036A).

    RNA Extraction:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Polymerase Chain Reaction:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: .. Total RNA was extracted using an RNA extraction kit (RC112, Vazyme, China) and reverse-transcribed into cDNA with a PCR kit (RR036A, Takara, Japan). .. Amplification reactions were carried out using the SYBR Green method on a real-time PCR system (Bio-Rad, USA).

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    SYBR Green Assay:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: Hydrogels were transferred to TRIzol (Thermo Fisher Scientific), crushed, and homogenized for phenol-chloroform RNA extraction and reverse transcript PCR (RT-PCR). .. Following preparation of cDNA using Primescript RT Master Mix (Takara Bio) in a MiniAmp Plus Thermal Cycler, q-PCR was conducted using Power SYBR Green PCR Master Mix (Applied Biosciences) and an Applied Biosystems QuantStudio 6 Pro Real-Time PCR system. ..

    Article Title: Intelligent-responsive hydrogel synergistically mediates immune remodel-antibacterial-angiogenesis cascade for diabetic foot ulcer repair
    Article Snippet: .. Methyl alcohol, Dimethyl sulfoxide (DMSO; General-Reagent, Titan, Shanghai, China); Deferoxamine mesylate (DFO; MCE, New Jersey, USA); Zn(NO 3 ) 2 ·6H 2 O, Mn(NO 3 ) 2 ·4H 2 O, 2-methylimidazole (Aladdin, Shanghai, China); UCMSC-exo (Langfang Kangbao Huitai Biotechnology, Langfang, China); Chitosan (CS; Deacetylation degree ≥95%, Macklin, Shanghai, China), 4-carboxyphenylboronic acid; polyvinyl alcohol (4-CPBA, PVA; Aladdin, Shanghai, China); N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC; Aladdin, Shanghai, China); N-Hydroxysuccinimide (NHS; Aladdin, Shanghai, China); PrimeScript RT Master Mix (Takara, Beijing, China); qPCR SYBR Green Master Mix (Yeasen, Shanghai, China); CD9 antibody (Selleck, USA); TSG101 antibody (Abmart, Shanghai, China); CD86 flow antibody and CD206 flow antibody (BioLegend, USA); IL-6, CD206, HIF-1α, CK14, Ki67, CD31, VEGF and α-SMA (AiFang biological, China). ..



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    Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of <t>RNA‐LNPs</t> by dynamic light scattering. (c) qRT‐PCR quantification of the <t>transcription</t> efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.
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    Image Search Results


    Transcriptomic analysis of B. cinerea strains TB-31, △ bcaba3 , and △ bcaba1234 . (A)∼(B) Regression analysis between transcriptomic data (RNA-seq) and RT‒qPCR validation. △ bcaba3 vs. TB-31: R 2 = 0.94, P < 0.001 (A); △ bcaba1234 vs. TB-31: R 2 = 0.97, P < 0.001 (B). (C) Heatmap of the relative transcriptional levels of the genes in the MVA pathway in △ bcaba1234 compared with those in TB-31. Gene transcription levels were normalized using Z score transformation, calculated as (X-mean)/SD, to represent the number of standard deviations above or below the mean transcription level. Upregulated genes are highlighted in red, while downregulated genes are shown in blue. The data are derived from three independent biological replicates. (D) Heatmap of the relative transcriptional levels of DEGs involved in other secondary metabolism pathways in △ bcaba1234 compared with those in TB-31. (E) Heatmap of the relative transcriptional levels of genes involved in intracellular primary metabolic pathways in the △ bcaba1234 strain compared with those in the TB-31 strain. Metabolic pathways are marked with gray boxes. PPP: pentose phosphate pathway; TCA: tricarboxylic acid cycle; GYC: glyoxylate cycle.

    Journal: Synthetic and Systems Biotechnology

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system

    doi: 10.1016/j.synbio.2025.12.002

    Figure Lengend Snippet: Transcriptomic analysis of B. cinerea strains TB-31, △ bcaba3 , and △ bcaba1234 . (A)∼(B) Regression analysis between transcriptomic data (RNA-seq) and RT‒qPCR validation. △ bcaba3 vs. TB-31: R 2 = 0.94, P < 0.001 (A); △ bcaba1234 vs. TB-31: R 2 = 0.97, P < 0.001 (B). (C) Heatmap of the relative transcriptional levels of the genes in the MVA pathway in △ bcaba1234 compared with those in TB-31. Gene transcription levels were normalized using Z score transformation, calculated as (X-mean)/SD, to represent the number of standard deviations above or below the mean transcription level. Upregulated genes are highlighted in red, while downregulated genes are shown in blue. The data are derived from three independent biological replicates. (D) Heatmap of the relative transcriptional levels of DEGs involved in other secondary metabolism pathways in △ bcaba1234 compared with those in TB-31. (E) Heatmap of the relative transcriptional levels of genes involved in intracellular primary metabolic pathways in the △ bcaba1234 strain compared with those in the TB-31 strain. Metabolic pathways are marked with gray boxes. PPP: pentose phosphate pathway; TCA: tricarboxylic acid cycle; GYC: glyoxylate cycle.

    Article Snippet: The sgRNAs were transcribed using the T7 High Yield RNA Transcription Kit (Nanjing Vazyme Biotech Co., Ltd., Nanjing, China), and then purified using the RNA Clean & Concentrator Kit (Zymo Research Corp., Irvine, California, USA).

    Techniques: RNA Sequencing, Biomarker Discovery, Transformation Assay, Derivative Assay

    Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

    Journal: Transboundary and Emerging Diseases

    Article Title: A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea

    doi: 10.1155/tbed/3115893

    Figure Lengend Snippet: Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

    Article Snippet: The transcription reaction consisted of linearized template DNA, N1‐methylpseudouridine‐5 ′ ‐triphosphate (Novoprotein, China), CAP GAU m7G(5 ′ )ppp(5")(2 ′ 0MeA)pU (SYNTHGENE, China), and components from the T7 High‐Yield RNA Transcription kit (Novoprotein, China).

    Techniques: Vaccines, Quantitative RT-PCR, Transfection, Western Blot, Expressing