Review



mirna microarray  (HTG Molecular)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    HTG Molecular mirna microarray
    ( A ) <t>qNPA</t> <t>microarray</t> was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each <t>miRNA</t> based on relative expression across samples.
    Mirna Microarray, supplied by HTG Molecular, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/mirna+microarray/pmc03507297-135-2-13
    Average 90 stars, based on 1 article reviews
    mirna microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Effects of β4 integrin expression on microRNA patterns in breast cancer"

    Article Title: Effects of β4 integrin expression on microRNA patterns in breast cancer

    Journal: Biology Open

    doi: 10.1242/bio.20121628

    ( A ) qNPA microarray was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each miRNA based on relative expression across samples.
    Figure Legend Snippet: ( A ) qNPA microarray was performed in triplicate on MCF10CA1a siCtrl cells and MCF10CA1a siβ4 cells at 72 hours post-transfection. The heat map depicts the 44 miRNAs undergoing a statistically significant change in expression following transient depletion of β4 subunit in this system. ( B ) qNPA microarray was performed in triplicate on two subclones of the MDA-MB-435/β4 transfectants (3A7 and 5B3), and two subclones of the MDA-MB-435/mock transfectants (6D2 and 6D7). The heat map depicts the 50 miRNAs undergoing a statistically significant change in expression following introduction of the β4 subunit into this system. ( C ) qNPA microarray was performed in triplicate on ten β4 positive and ten β4 negative invasive breast carcinomas. The heat map depicts the 74 miRNAs differentially expressed between tumor subsets. For all array analyses, a p-value < 0.05 and a ±1.2-fold change cut-off was applied. Color was assigned to each miRNA based on relative expression across samples.

    Techniques Used: Microarray, Transfection, Expressing


    Figure Legend Snippet: Effect of β4 expression on miRNA levels.

    Techniques Used: Expressing

    ( A ) Venn diagram of overlapping miRNAs that undergo differential expression in response to β4 across all three arrays. ( B ) Venn diagram of overlapping miRNA families that undergo differential expression in response to β4 across all three arrays.
    Figure Legend Snippet: ( A ) Venn diagram of overlapping miRNAs that undergo differential expression in response to β4 across all three arrays. ( B ) Venn diagram of overlapping miRNA families that undergo differential expression in response to β4 across all three arrays.

    Techniques Used: Quantitative Proteomics


    Figure Legend Snippet: Effect of β4 expression on miRNA families.

    Techniques Used: Expressing

    GeneChip derived mRNA levels were ranked from the most upregulated in β4 transfected cells to the most downregulated (x-axis, 1 to 12,300, respectively). Red shading indicates mRNA is upregulated in β4 transfectants, while blue shading indicates mRNA is downregulated. Each vertical black line represents a miRNA target. The left-to-right position of each black line indicates the relative position of the predicted target within the rank ordered mRNA list. ( A ) miR-92ab predicted target gene are enriched among mRNAs up-regulated in the β4 transfectants, as illustrated by the increasing number of black lines on the left side of each graphic and the positive running enrichment scores (ES) marked by the red lines (p = 0.028). No enrichment was detected for and miR-99ab/100. ( B ) miR-15abc/16/16abc/195/322/424/497/1907 (p = 0.039), miR-23abc/23b-3p (p = 0/034), miR-27abc/27a-3p (p = 0.003), and miR-30abcdef/30abe-5p/384-5p (p = 0.0) predicted target genes are enriched among mRNAs up-regulated in the β4 transfectants.
    Figure Legend Snippet: GeneChip derived mRNA levels were ranked from the most upregulated in β4 transfected cells to the most downregulated (x-axis, 1 to 12,300, respectively). Red shading indicates mRNA is upregulated in β4 transfectants, while blue shading indicates mRNA is downregulated. Each vertical black line represents a miRNA target. The left-to-right position of each black line indicates the relative position of the predicted target within the rank ordered mRNA list. ( A ) miR-92ab predicted target gene are enriched among mRNAs up-regulated in the β4 transfectants, as illustrated by the increasing number of black lines on the left side of each graphic and the positive running enrichment scores (ES) marked by the red lines (p = 0.028). No enrichment was detected for and miR-99ab/100. ( B ) miR-15abc/16/16abc/195/322/424/497/1907 (p = 0.039), miR-23abc/23b-3p (p = 0/034), miR-27abc/27a-3p (p = 0.003), and miR-30abcdef/30abe-5p/384-5p (p = 0.0) predicted target genes are enriched among mRNAs up-regulated in the β4 transfectants.

    Techniques Used: Derivative Assay, Transfection

    Related Articles

    Microarray:

    Article Title: Effects of β4 integrin expression on microRNA patterns in breast cancer
    Article Snippet: .. A novel qNPA based miRNA Microarray high throughput platform from High Throughput Genomics (HTG Molecular Diagnostics, Inc.; Tuscon, AZ, USA) was used to study 1050 mature miRNAs in human, rat, and mouse based upon the Sanger miRBase release 9.1. .. The qNPA based miRNA microarrays comprise DNA oligo capture probes that are synthesized directly on the slide surface (Roche NimbleGen, Madison, WI, USA) which are complementary to, and capture, biotinylated miRNA-specific nuclease protection probes.

    High Throughput Screening Assay:

    Article Title: Effects of β4 integrin expression on microRNA patterns in breast cancer
    Article Snippet: .. A novel qNPA based miRNA Microarray high throughput platform from High Throughput Genomics (HTG Molecular Diagnostics, Inc.; Tuscon, AZ, USA) was used to study 1050 mature miRNAs in human, rat, and mouse based upon the Sanger miRBase release 9.1. .. The qNPA based miRNA microarrays comprise DNA oligo capture probes that are synthesized directly on the slide surface (Roche NimbleGen, Madison, WI, USA) which are complementary to, and capture, biotinylated miRNA-specific nuclease protection probes.



    Similar Products

    micro-rna microarray platform  (Agilent technologies)
    90
    Agilent technologies micro-rna microarray platform
    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.
    Micro Rna Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/pmc03804513-22-8-7
    Average 90 stars, based on 1 article reviews
    micro-rna microarray platform - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human m6a small rna epitranscriptomic microarray platform  (Arraystar inc)
    90
    Arraystar inc human m6a small rna epitranscriptomic microarray platform
    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.
    Human M6a Small Rna Epitranscriptomic Microarray Platform, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/human+mrna+lncrna+epitranscriptomic+microarray/pm39752904-226-8-7
    Average 90 stars, based on 1 article reviews
    human m6a small rna epitranscriptomic microarray platform - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human microarray platform  (TaKaRa)
    96
    TaKaRa human microarray platform
    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.
    Human Microarray Platform, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/SMARTer+Universal+Low+Input+RNA+Kit+for+Sequencing/pm37925448-197-14-26
    Average 96 stars, based on 1 article reviews
    human microarray platform - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    rna microarray platform  (Illumina Inc)
    90
    Illumina Inc rna microarray platform
    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from <t>microarray</t> data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.
    Rna Microarray Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/rna+microarray+platform/pmc07226539-88-15-14
    Average 90 stars, based on 1 article reviews
    rna microarray platform - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    multiplex rna microarray platform midtal  (Microbia Inc)
    90
    Microbia Inc multiplex rna microarray platform midtal
    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from <t>microarray</t> data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.
    Multiplex Rna Microarray Platform Midtal, supplied by Microbia Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/midtal+microarray/pm28427011-90-8-17
    Average 90 stars, based on 1 article reviews
    multiplex rna microarray platform midtal - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human long noncoding rna 4 × 180k microarray platform  (Agilent technologies)
    90
    Agilent technologies human long noncoding rna 4 × 180k microarray platform
    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from <t>microarray</t> data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.
    Human Long Noncoding Rna 4 × 180k Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/pmc04757888-111-1-0
    Average 90 stars, based on 1 article reviews
    human long noncoding rna 4 × 180k microarray platform - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

    Journal: PLoS ONE

    Article Title: Scleral Micro-RNA Signatures in Adult and Fetal Eyes

    doi: 10.1371/journal.pone.0078984

    Figure Lengend Snippet: Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

    Article Snippet: Genome-wide micro-RNA profiling was performed using the Agilent micro-RNA microarray platform.

    Techniques: Microarray

    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

    Journal: Cancers

    Article Title: Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma

    doi: 10.3390/cancers12041057

    Figure Lengend Snippet: ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

    Article Snippet: Gene expression profiling was performed in four Merkel cell carcinoma cell lines, using the Illumina RNA microarray platform.

    Techniques: Microarray, Expressing, Western Blot, Over Expression, Colony Assay