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human m6a small rna epitranscriptomic microarray platform  (Arraystar inc)

 
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    Arraystar inc human m6a small rna epitranscriptomic microarray platform
    Human M6a Small Rna Epitranscriptomic Microarray Platform, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+microarray+platform/human+mrna+lncrna+epitranscriptomic+microarray/pm39752904-226-8-7
    Average 90 stars, based on 1 article reviews
    human m6a small rna epitranscriptomic microarray platform - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: N6-methyladenosine regulates metabolic remodeling in kidney aging through transcriptional regulator GLIS1
    Article Snippet: .. The RNAs were then hybridized to the Arraystar Human mRNA Epitranscriptomic Microarray (8 × 60 K, Arraystar). ..

    Article Title: N6-methyladenosine regulates metabolic remodeling in kidney aging through transcriptional regulator GLIS1
    Article Snippet: .. Human m6A epitranscriptomic microarray and mRNA microarray analyses were performed based on Arraystar’s standard protocols. ..

    Article Title: METTL3 promotes human amniotic epithelial stem cells differentiation into insulin-producing cells by regulation of MaFA expression.
    Article Snippet: Objective: Generating mature β-cells from stem cells remains a significant challenge in diabetes cell therapy.. Human amniotic epithelial stem cells (hAESCs) have made their mark in regenerative medicine, and provide several advantages compared to other stem cells.. Methyltransferase-like 3 (METTL3), an essential RNA methyltransferase participating in N6-methyladenosine (m6A) mRNA methylation, plays a critical role in the normal development of β-cells, yet its deletion in β-cells leads to β-cell dysfunction and hyperglycemia.

    Article Title: WTAP/YTHDF1-mediated m 6 A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs
    Article Snippet: .. In the hybridization step, the cRNA was merged to the Arraystar Human m 6 A Epitranscriptomic Microarray (8 × 60 K; Arraystar, Rockville, USA). .. Finally, the array was scanned using an Agilent G2505C scanner.

    Article Title: Linking oxysterols and different stages of mild cognitive impairment: insights from gut metabolites and N6-methyladenosine
    Article Snippet: The “IP” and “Sup” RNAs were labeled with Cy5 and Cy3 respectively as cRNAs in separate reactions using Arraystar RNA Labeling Protocol. .. The cRNAs were combined and hybridized onto Arraystar Human mRNA Epitranscriptomic Microarray (8 × 60 K, Arraystar). .. After washing the slides, the arrays were scanned in two-color channels by an Agilent Scanner G2505C.

    other:

    Article Title: Noncoding RNA and Alcohol Use Disorder: A Scoping Review of Current Research and Knowledge Gaps
    Article Snippet: Human , M , Brain , miRNeasy mini-Kit , Arraystar m6AmRNA & lncRNA Epitranscriptomic Microarray Assay , 29 mRNAs, five lncRNAs, and three miRNAs were differentially methylated (|FC| ≥ 2 and p < 0.05) in people with AUD. , Liu, 2022 .

    Hybridization:

    Article Title: METTL3 promotes human amniotic epithelial stem cells differentiation into insulin-producing cells by regulation of MaFA expression.
    Article Snippet: Objective: Generating mature β-cells from stem cells remains a significant challenge in diabetes cell therapy.. Human amniotic epithelial stem cells (hAESCs) have made their mark in regenerative medicine, and provide several advantages compared to other stem cells.. Methyltransferase-like 3 (METTL3), an essential RNA methyltransferase participating in N6-methyladenosine (m6A) mRNA methylation, plays a critical role in the normal development of β-cells, yet its deletion in β-cells leads to β-cell dysfunction and hyperglycemia.

    Article Title: WTAP/YTHDF1-mediated m 6 A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs
    Article Snippet: .. In the hybridization step, the cRNA was merged to the Arraystar Human m 6 A Epitranscriptomic Microarray (8 × 60 K; Arraystar, Rockville, USA). .. Finally, the array was scanned using an Agilent G2505C scanner.

    Methylation:

    Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway
    Article Snippet: .. In this research, we applied Arraystar m 6 A lncRNA epitranscriptomic microarrays to screen differentially m 6 A-methylated lncRNAs in lung cancer and adjacent tissues and then identified the priority candidate genes SCIRT. m 6 A methylation and SCIRT expression were upregulated in a METTL3-dependent manner in NSCLC cells. ..

    Expressing:

    Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway
    Article Snippet: .. In this research, we applied Arraystar m 6 A lncRNA epitranscriptomic microarrays to screen differentially m 6 A-methylated lncRNAs in lung cancer and adjacent tissues and then identified the priority candidate genes SCIRT. m 6 A methylation and SCIRT expression were upregulated in a METTL3-dependent manner in NSCLC cells. ..



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    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.
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    Image Search Results


    Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

    Journal: PLoS ONE

    Article Title: Scleral Micro-RNA Signatures in Adult and Fetal Eyes

    doi: 10.1371/journal.pone.0078984

    Figure Lengend Snippet: Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

    Article Snippet: Genome-wide micro-RNA profiling was performed using the Agilent micro-RNA microarray platform.

    Techniques: Microarray

    ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

    Journal: Cancers

    Article Title: Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma

    doi: 10.3390/cancers12041057

    Figure Lengend Snippet: ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

    Article Snippet: Gene expression profiling was performed in four Merkel cell carcinoma cell lines, using the Illumina RNA microarray platform.

    Techniques: Microarray, Expressing, Western Blot, Over Expression, Colony Assay