Review




Structured Review

CapitalBio Corporation mirna microarray detection platform
Experimental variation and reproducibility assessment from twelve <t>microarray</t> hybridizations in six different samples . Scatter diagram showing high reproducibility between the replicate experiments of every sample. The R-value in each microarray analysis showing that most of the average correlations are well above 0.9, indicating high reproducibility. Panel A~C: self-hybridization results obtained after probing the microarray with the same RNA sample prepared from three normal tissues and labeled separately with Cy3 dye. Panel D~F: self-hybridization results obtained after probing the microarray with the same RNA sample prepared from three cancer tissues and labeled separately with Cy3 dye.
Mirna Microarray Detection Platform, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+microarray+platform/mirna+microarray/pmc02687417-109-1-8
Average 90 stars, based on 1 article reviews
mirna microarray detection platform - by Bioz Stars, 2026-09
90/100 stars

Images

1) Product Images from "The expression profile of microRNAs in a model of 7,12-dimethyl-benz[ a ]anthrance-induced oral carcinogenesis in Syrian hamster"

Article Title: The expression profile of microRNAs in a model of 7,12-dimethyl-benz[ a ]anthrance-induced oral carcinogenesis in Syrian hamster

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/1756-9966-28-64

Experimental variation and reproducibility assessment from twelve microarray hybridizations in six different samples . Scatter diagram showing high reproducibility between the replicate experiments of every sample. The R-value in each microarray analysis showing that most of the average correlations are well above 0.9, indicating high reproducibility. Panel A~C: self-hybridization results obtained after probing the microarray with the same RNA sample prepared from three normal tissues and labeled separately with Cy3 dye. Panel D~F: self-hybridization results obtained after probing the microarray with the same RNA sample prepared from three cancer tissues and labeled separately with Cy3 dye.
Figure Legend Snippet: Experimental variation and reproducibility assessment from twelve microarray hybridizations in six different samples . Scatter diagram showing high reproducibility between the replicate experiments of every sample. The R-value in each microarray analysis showing that most of the average correlations are well above 0.9, indicating high reproducibility. Panel A~C: self-hybridization results obtained after probing the microarray with the same RNA sample prepared from three normal tissues and labeled separately with Cy3 dye. Panel D~F: self-hybridization results obtained after probing the microarray with the same RNA sample prepared from three cancer tissues and labeled separately with Cy3 dye.

Techniques Used: Microarray, Hybridization, Labeling

Supervised hierarchical clustering analysis of miRNA expression . 17 miRNAs expression profile (from SAM result) of 6 samples were clustered using Cluster 3.0. 6 samples were successfully separated into 2 discrete groups.
Figure Legend Snippet: Supervised hierarchical clustering analysis of miRNA expression . 17 miRNAs expression profile (from SAM result) of 6 samples were clustered using Cluster 3.0. 6 samples were successfully separated into 2 discrete groups.

Techniques Used: Expressing

MicroRNAs  microarray  SAM results and correlation with cancer
Figure Legend Snippet: MicroRNAs microarray SAM results and correlation with cancer

Techniques Used: Microarray

Related Articles

other:

Article Title: Regulatory effects of miR-28 on osteogenic differentiation of human bone marrow mesenchymal stem cells
Article Snippet: TRIzol solution was from Invitrogen (USA), miRNA Isolation Kits were from Roche (Switzerland), and miRNA microarray V2.0 was purchased from Beijing CapitalBio Corporation (China; 576 miRNAs labeled in human, 238 in rats, and 358 in mice).

Expressing:

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps
Article Snippet: .. MiRNA expression profile microarrays of these specimens were performed by CapitalBio. ..

Control:

Article Title: Oncogenic miR-20b-5p contributes to malignant behaviors of breast cancer stem cells by bidirectionally regulating CCND1 and E2F1.
Article Snippet: CD44+CD24−/low BCSCs were isolated from MCF-7 and T47D cells by staining with CD44-APC, CD24-PE and ESA-FITC (BD Pharmingen, USA) antibodies via FACS as described in our previous research [8, 20]. miRNA profile and miRNA network Both human miRNA microarray fabrication and hybridization were performed as described previously [8]. .. The miRNA profiles of both BCSCs and control MCF-7 breast cancer cells were obtained from CapitalBio Corporation (Beijing, China). ..

Purification:

Article Title: MiR-672-5p-Mediated Upregulation of REEP6 in Spinal Dorsal Horn Participates in Bortezomib-Induced Neuropathic Pain in Rats.
Article Snippet: Evidence shows that miRNAs are deeply involved in nervous system diseases, but whether miRNAs contribute to the bortezomib (BTZ)-induced neuropathic pain remains unclear.. We aimed to investigate whether miRNAs contribute to bortezomib (BTZ)-induced neuropathic pain and explore the related downstream cascades.. The level of miRNAs in the spinal dorsal horn was explored using miRNA microarray and PCR.

Labeling:

Article Title: MiR-672-5p-Mediated Upregulation of REEP6 in Spinal Dorsal Horn Participates in Bortezomib-Induced Neuropathic Pain in Rats.
Article Snippet: Evidence shows that miRNAs are deeply involved in nervous system diseases, but whether miRNAs contribute to the bortezomib (BTZ)-induced neuropathic pain remains unclear.. We aimed to investigate whether miRNAs contribute to bortezomib (BTZ)-induced neuropathic pain and explore the related downstream cascades.. The level of miRNAs in the spinal dorsal horn was explored using miRNA microarray and PCR.

Hybridization:

Article Title: MiR-672-5p-Mediated Upregulation of REEP6 in Spinal Dorsal Horn Participates in Bortezomib-Induced Neuropathic Pain in Rats.
Article Snippet: Evidence shows that miRNAs are deeply involved in nervous system diseases, but whether miRNAs contribute to the bortezomib (BTZ)-induced neuropathic pain remains unclear.. We aimed to investigate whether miRNAs contribute to bortezomib (BTZ)-induced neuropathic pain and explore the related downstream cascades.. The level of miRNAs in the spinal dorsal horn was explored using miRNA microarray and PCR.

Microarray:

Article Title: MiR-672-5p-Mediated Upregulation of REEP6 in Spinal Dorsal Horn Participates in Bortezomib-Induced Neuropathic Pain in Rats.
Article Snippet: Evidence shows that miRNAs are deeply involved in nervous system diseases, but whether miRNAs contribute to the bortezomib (BTZ)-induced neuropathic pain remains unclear.. We aimed to investigate whether miRNAs contribute to bortezomib (BTZ)-induced neuropathic pain and explore the related downstream cascades.. The level of miRNAs in the spinal dorsal horn was explored using miRNA microarray and PCR.

Article Title: MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell.
Article Snippet: The total RNAs were isolated from 6 CC tissues and 6 PC tissues in according to the protocol of TRIzol reagent (15,596,026, Invitrogen, USA) and purified using mirVanaTM miRNA Isolation Kit (AM1561, Life Technologies, USA). .. Then, the differential expression of RNA was identified using miRNA microarray by CapitalBio Corporation (CapitalBio, China). ..

Article Title: Retracted: Transcription activation of microRNA-25 by PEA3 augments progression of gastric cancer through suppressing SIK1.
Article Snippet: .. A miRNA microarray analysis was performed by CapitalBio (Capital BioCorp, Beijing China) using tumor and the paired normal tissue samples from three patients. .. Total RNA from tissues was collected by TRIzol Reagent (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Article Title: miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis
Article Snippet: .. Then, the microarray analysis of miRNAs was performed by CapitalBio Corporation following the protocol as previously described ( ). ..

Quantitative Proteomics:

Article Title: MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell.
Article Snippet: The total RNAs were isolated from 6 CC tissues and 6 PC tissues in according to the protocol of TRIzol reagent (15,596,026, Invitrogen, USA) and purified using mirVanaTM miRNA Isolation Kit (AM1561, Life Technologies, USA). .. Then, the differential expression of RNA was identified using miRNA microarray by CapitalBio Corporation (CapitalBio, China). ..



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Image Search Results


Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

Journal: PLoS ONE

Article Title: Scleral Micro-RNA Signatures in Adult and Fetal Eyes

doi: 10.1371/journal.pone.0078984

Figure Lengend Snippet: Mean 2^-deltaCt values and corresponding standard deviations (SD) for posterior and peripheral scleral samples from adult and fetal eyes; adult versus fetal eye fold differences and related P-values listed for the same samples.

Article Snippet: Genome-wide micro-RNA profiling was performed using the Agilent micro-RNA microarray platform.

Techniques: Microarray

( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

Journal: Cancers

Article Title: Unique Role of Histone Methyltransferase PRDM8 in the Tumorigenesis of Virus-Negative Merkel Cell Carcinoma

doi: 10.3390/cancers12041057

Figure Lengend Snippet: ( A ) Heatmap shows differentially expressed microRNA (miRNAs) in VP- vs. VN-MCC cell lines from microarray data analysis (false discovery rate ( FDR ) <0.05). ( B ) miRNA pathway analysis using mirPath v.3 in the top differentially expressed miRNAs, showing enriched pathways (i.e., viral carcinogenesis and miRNAs involved in cancer). ( C ) Intersection of top upregulated miRNAs in VP-MCC with top miRNAs that could potentially target PRDM8 (scored using miRWalk) indicates miR-20a-5p as the only miRNA that can target PRDM8 and at the same time is downregulated in VN-MCCs. ( D ) Overexpressing miR-20a-5p using miRNA mimic decreases PRDM8 expression level significantly in MCC13 cells. ( E ) Immunoblot shows that PRDM8 protein levels are decreased upon miR-20a-5p overexpression. Ectopic overexpression of miR-20a-5p also led to the decrease in H3K9me3 levels. Histone H3 is used as the control. ( F ) Colony formation assay performed in MCC13 cells compared to miR-20a-5p-overexpressed MCC13 cells indicated lower clonogenicity capability upon overexpression of miR-20a-5p. Representative figures of colonies in MCC13 cells are shown. * p -value < 0.05.

Article Snippet: Gene expression profiling was performed in four Merkel cell carcinoma cell lines, using the Illumina RNA microarray platform.

Techniques: Microarray, Expressing, Western Blot, Over Expression, Colony Assay